Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof

1. A single chamber rolling bottle comprising primate pluripotent-derived cell aggregates in suspension in a physiologically acceptable medium, wherein the primate pluripotent-derived cell aggregates are derived from a single cell suspension of primate pluripotent stem cells agitated by rotation at a speed between about 3 to about 20 rpm.

2. The single chamber rolling bottle of claim 1 , wherein the primate pluripotent-derived cell aggregates are formed in the roller bottle.

3. The single chamber rolling bottle of claim 1 , wherein the primate pluripotent-derived cell aggregates are substantially definitive endoderm lineage cell aggregates.

4. The single chamber rolling bottle of claim 3 , wherein the definitive endoderm lineage cell aggregates are substantially pancreatic endoderm cells or are substantially definitive endoderm cells.

5. The single chamber rolling bottle of claim 3 , wherein the definitive endoderm lineage cell aggregates express at least one marker selected from the group consisting of Sox17, HNF3β, HNF1β, PDX1, NKX6.1, PTF1A, NGN3 and NKX2.2.

6. The single chamber rolling bottle of claim 3 , wherein the definitive endoderm lineage cell aggregates do not significantly express PAX6, SOX7 or ZIC1.

7. The single chamber rolling bottle of claim 1 , further comprising an effective amount of a retinoic acid receptor (RAR) agonist.

8. The single chamber rolling bottle of claim 1 , wherein the primate pluripotent-derived cell aggregates are also agitated by rotation at a speed between about 3 to about 20 rpm.

9. The single chamber rolling bottle of claim 1 , wherein the primate pluripotent-derived-cell aggregates are substantially uniform in size and shape.

10. The single chamber rolling bottle of claim 1 , wherein the primate pluripotent-derived cell aggregates expand after contact with an effective amount of noggin.

11. The single chamber rolling bottle of claim 3 , wherein the definitive endoderm lineage cell aggregates are agitated by laminar or streamline flow.

12. The single chamber rolling bottle of claim 1 , wherein the primate pluripotent-derived cell aggregates have a diameter from about 50 microns to about 250 microns.

13. A method for preparing a single chamber rolling bottle containing primate pluripotent-derived cell aggregates in suspension, comprising:

(a) providing a single chamber roller bottle containing primate pluripotent cell aggregates derived from a single cell suspension of primate pluripotent stem cells; and

(b) contacting the primate pluripotent cell aggregates with a differentiation agent thereby generating a rolling bottle comprising primate pluripotent-derived cell aggregates in suspension,

wherein the primate pluripotent-derived cell aggregates are agitated by rotation at a speed between about 3 to about 20 rpm.

14. The method of claim 13 , wherein the primate pluripotent-derived cell aggregates express at least one marker selected from the group consisting of Sox17, HNF3β, HNF1β, PDX1, NKX6.1, PTF1A, NGN3 and NKX2.2.

15. The method of claim 13 , wherein the primate pluripotent-derived-cell aggregates do not significantly express PAX6, SOX7 or ZIC1.

16. The method of claim 13 , wherein the primate pluripotent-derived cell aggregates are generated by laminar or streamline flow.

17. The method of claim 13 , wherein the primate pluripotent-derived cell aggregates are substantially definitive endoderm lineage cell aggregates.

18. The method of claim 17 , wherein the definitive endoderm lineage cell aggregates are substantially pancreatic endoderm cells or are substantially definitive endoderm cells.

19. The method of claim 13 , wherein the primate pluripotent-derived cell aggregates have a diameter from about 50 microns to about 250 microns.

20. The method of claim 13 , wherein the primate pluripotent-cell aggregates are substantially uniform in size and shape.