Subfamily E simian adenoviruses A1321, A1325, A1295, A1309, A1316 and A1322 and uses thereof
Recombinant vectors comprise simian adenovirus A1321 (SAdV-A1321), SAdV-A1325, SAdV-A1295, SAdV-A1309, SAdV-A1316, and/or SAdV-A1322 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus SAdV-A1321, SAdV-A1325, SAdV-A1295, SAdV-A1309, SAdV-A1316, and/or SAdV-A1322 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.
1. An adenovirus having a capsid comprising a hexon protein, a penton protein, and a fiber protein, wherein said hexon protein is the hexon protein of SAdV-A1321 with the amino acids 1 to 942 of SEQ ID NO: 10, and wherein said penton protein is the penton protein of SAdV-A1321 with the amino acids 1 to 540 of SEQ ID NO: 5; said capsid encapsidating a heterologous nucleic acid comprising a gene operably linked to expression control sequences which direct transcription, translation, and/or expression thereof in a host cell.
2. The adenovirus according to claim 1 , further comprising a 5′ and a 3′ adenovirus cis-element necessary for replication and encapsidation.
3. The adenovirus according to claim 1 , wherein said adenovirus lacks all or a part of the E1 gene.
4. The adenovirus according to claim 3 , wherein said adenovirus is replication-defective.
5. The adenovirus according to claim 1 , wherein said fiber protein is the fiber protein of SAdV-A1321 with the amino acids 1 to 445 of SEQ ID NO: 19.
6. The adenovirus according to claim 1 , wherein said capsid is a hybrid capsid.
7. The adenovirus according to claim 6 , wherein said hybrid capsid comprises at least one capsid protein from an adenovirus selected from SAdV-A1316, SAdV-1322, and SAdV-A1325.
8. A composition comprising the adenovirus according to claim 1 in a pharmaceutically acceptable carrier.
9. A composition comprising an adenovirus according to claim 1 , said adenovirus further comprising at least one simian adenovirus protein selected from the group consisting of:
E1a, SEQ ID NO: 27;
E1b, small T/19K, SEQ ID NO: 2;
E1b, large T/55K, SEQ ID NO: 21;
IX, SEQ ID NO: 3;
IIIa, SEQ ID NO: 4;
VII, SEQ ID NO: 6;
V, SEQ ID NO: 7;
pX, SEQ ID NO: 8;
VI, SEQ ID NO: 9;
Endoprotease, SEQ ID NO: 11;
100 kD, SEQ ID NO: 12;
22 kD, SEQ ID NO: 22;
VIII, SEQ ID NO: 13;
CR1-alpha SEQ ID NO: 14;
gp19K, SEQ ID NO: 23;
CR1-beta, SEQ ID NO: 15;
CR1-gamma, SEQ ID NO: 16;
CR1-delta, SEQ ID NO: 24;
RID-alpha, SEQ ID NO: 17;
RID-beta, SEQ ID NO: 18;
E3/14.7K, SEQ ID NO: 25; and
Fiber, SEQ ID NO: 19.
10. An adenovirus having a capsid comprising a hexon protein, a penton protein, and a fiber protein, wherein said hexon protein is the hexon protein of SAdV-A1321 with the amino acids 1 to 942 of SEQ ID NO: 10, and wherein said fiber protein is the fiber protein of SAdV-A1321 with the amino acids 1 to 445 of SEQ ID NO: 19; said capsid encapsidating a heterologous nucleic acid comprising a gene operably linked to expression control sequences which direct transcription, translation, and/or expression thereof in a host cell.
11. The adenovirus according to claim 10 , further comprising a 5′ and a 3′ adenovirus cis-element necessary for replication and encapsidation.
12. The adenovirus according to claim 10 , wherein said adenovirus lacks all or a part of the E1 gene.
13. The adenovirus according to claim 12 , wherein said adenovirus is replication defective.
14. The adenovirus according to claim 10 , wherein said capsid is a hybrid capsid.
15. The adenovirus according to claim 14 , wherein said hybrid capsid comprises at least one capsid protein from an adenovirus selected from SAdV-A1316, SAdV-1322, and SAdV-A1325.
16. A composition comprising the adenovirus according to claim 10 in a pharmaceutically acceptable carrier.
17. A composition comprising an adenovirus according to claim 10 , said adenovirus further comprising at least one simian adenovirus protein selected from the group consisting of:
E1a, SEQ ID NO: 27;
E1b, small T/19K, SEQ ID NO: 2;
E1b, large T/55K, SEQ ID NO: 21;
IX, SEQ ID NO: 3;
IIIa, SEQ ID NO: 4;
Penton, SEQ ID NO: 5;
VII, SEQ ID NO: 6;
V, SEQ ID NO: 7;
pX, SEQ ID NO: 8;
VI, SEQ ID NO: 9;
Endoprotease, SEQ ID NO: 11;
100 kD, SEQ ID NO: 12;
22 kD, SEQ ID NO: 22;
VIII, SEQ ID NO: 13;
CR1-alpha SEQ ID NO: 14;
gp19K, SEQ ID NO: 23;
CR1-beta, SEQ ID NO: 15;
CR1-gamma, SEQ ID NO: 16;
CR1-delta, SEQ ID NO: 24;
RID-alpha, SEQ ID NO: 17;
RID-beta, SEQ ID NO: 18; and
E3/14.7K, SEQ ID NO: 25.