BSL2v2c2-Ig polypeptides
The present invention provides nucleic acids encoding B7-related factors that modulate the activation of immune or inflammatory response cells, such as T-cells. Also provided are expression vectors and fusion constructs comprising nucleic acids encoding B7-related polypeptides, including BSL1, BSL2, and BSL3. The present invention further provides isolated B7-related polypeptides, isolated fusion proteins comprising B7-related polypeptides, and antibodies that are specifically reactive with B7-related polypeptides, or portions thereof. In addition, the present invention provides assays utilizing B7-related nucleic acids, polypeptides, or peptides. The present invention further provides compositions of B7-related nucleic acids, polypeptides, fusion proteins, or antibodies that are useful for the immunomodulation of a human or animal subject.
1. An isolated polypeptide comprising
(a) amino acids 1 to 480 of SEQ ID NO:135;
(b) amino acids 2 to 480 of SEQ ID NO:135; or
(c) amino acids 29 to 480 of SEQ ID NO:135.
2. The isolated polypeptide of claim 1 , wherein said polypeptide is (a).
3. The isolated polypeptide of claim 2 , wherein said polypeptide is encoded by nucleotides 1 to 1440 of SEQ ID NO:134.
4. The isolated polypeptide of claim 1 , wherein said polypeptide is (b).
5. The isolated polypeptide of claim 4 , wherein said polypeptide is encoded by nucleotides 4 to 1440 of SEQ ID NO:134.
6. The isolated polypeptide of claim 1 , wherein said polypeptide is (c).
7. The isolated polypeptide of claim 6 , wherein said polypeptide is encoded by nucleotides 85 to 1440 of SEQ ID NO:134.
8. An isolated polypeptide encoded by the isolated nucleic acid in ATCC Deposit No. PTA-1987.
9. An isolated polypeptide produced by a method comprising:
(a) culturing an isolated recombinant host cell comprising a vector that comprises the coding region encoding the polypeptide of claim 1 under conditions such that said polypeptide is expressed; and (b) recovering said polypeptide.
10. The isolated polypeptide of claim 1 wherein further comprising a heterologous polypeptide sequence.
11. An isolated polypeptide comprising amino acids 1 to 247 of SEQ ID NO:135 fused to the Fc domain of an immunoglobulin.
12. An isolated polypeptide comprising amino acids 29 to 246 of SEQ ID NO:135 fused to the Fc domain of an immunoglobulin.
13. An isolated polypeptide comprising:
(a) a first sequence comprising amino acids 1 to 246 of SEQ ID NO:135 fused to a second sequence comprising the Fc domain of an immunoglobulin;
(b) a first sequence comprising amino acids 2 to 246 of SEQ ID NO:135 fused to a second sequence comprising the Fc domain of an immunoglobulin; or
(c) a first sequence comprising amino acids 29 to 246 of SEQ ID NO:135 fused to a second sequence comprising the Fc domain of an immunoglobulin.
14. The isolated polypeptide of claim 13 , wherein said first sequence of (a) comprises a sequence encoded by nucleotides 1 to 738 of SEQ ID NO:134.
15. The isolated polypeptide of claim 13 , wherein said first sequence of (b) comprises a sequence encoded by nucleotides 4 to 738 of SEQ ID NO:134.
16. The isolated polypeptide of claim 13 , wherein said first sequence of (c) comprises a sequence encoded by nucleotides 85 to 738 of SEQ ID NO:134.
17. The isolated polypeptide according to claim 11 , 12 , 13 , 14 , 15 , or 16 , wherein said immunoglobulin is IgG 1 .
18. An isolated polypeptide comprising amino acids 29 to 246 of SEQ ID NO:135.
19. The isolated polypeptide of claim 18 , wherein said polypeptide comprises a sequence encoded by nucleotides 85 to 738 of SEQ ID NO:134.