IP Library › Granted Patent US 10,386,330
Granted Patent B2
US 10,386,330 · App. 16/297,555 · Granted Aug 20, 2019

Biochemical analysis instrument

Inventors: Clive Gavin Brown (Cambridge, GB); James Peter Willcocks (Oxford, GB)
Assignee: Oxford Nanopore Technologies Ltd.
G01N27/44756C12Q1/6869G01N33/48721G01N15/1459G01N15/1463
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Quick Facts
Patent No.
US 10,386,330
App. No.
16/297,555
Granted
Aug 20, 2019
Kind
B2
Abstract

An analysis instrument comprises plural modules connected together over a data network, each module comprising an analysis apparatus operable to perform biochemical analysis of a sample. Each module comprises a control unit that controls the operation of the analysis apparatus. The control units are addressable to select an arbitrary number of modules to operate as a cluster for performing a common biochemical analysis. The control units communicate over the data network, repeatedly during the performance of the common biochemical analysis, to determine the operation of the analysis apparatus of each module required to meet the global performance targets, on the basis of measures of performance derived from the output data produced by the modules. The arrangement of the instrument as modules interacting in this manner provides a scalable analysis instrument.

Claims (21)

1. An analysis instrument for performing biochemical analysis, the analysis instrument comprising a plurality of modules connected together over a data network, wherein: each module comprises an analysis apparatus that is operable to perform biochemical analysis of a sample, the module being configured to produce output data of at least one channel representing the results of the biochemical analysis, and the operation of the analysis apparatus being controllable in a manner that varies its performance; and the analysis apparatus comprises at least one flow cell; wherein the analysis instrument further comprises a control system that is configured to accept input selecting an arbitrary number of modules as a cluster for performing a common biochemical analysis and is configured to accept input representing global performance targets corresponding to the common biochemical analysis; and the control system is configured to determine, at least once during the performance of the common biochemical analysis, measures of performance of each module from the output data produced by the modules.

2. The analysis instrument of claim 1 , wherein the control system is configured to control the operation of the modules of the cluster to perform the common biochemical analysis.

3. The analysis instrument of claim 2 , wherein the control system comprises a control unit in each module that is operable to control the operation of that module.

4. The analysis instrument of claim 3 , wherein each control unit is arranged to present a user-interface over the network for a computer connected thereto.

5. The analysis instrument of claim 1 , wherein the measures of global performance targets are derived locally in the modules.

6. The analysis instrument of claim 1 , wherein the control system is configured to vary the control of the operation of the modules on the basis of the determined measures of performance of all the modules in the cluster and the global performance targets.

7. The analysis instrument of claim 1 , wherein the control system is configured to take remedial action in the event a measure of performance is not achievable during operation of a module in the cluster.

8. The analysis instrument of claim 7 , wherein the remedial action is stopping the biochemical analysis.

9. The analysis instrument of claim 1 , wherein the measure of performance of each module is the status of producing output data.

10. The analysis instrument of claim 1 , wherein the measure of performance of each module is the quantity or quality of output data.

11. The analysis instrument of claim 1 , wherein the control system is configured to control the operation of each flow cell in a manner that varies its performance.

12. The analysis instrument of claim 1 , wherein each flow cell comprises a zero mode waveguide.

13. The analysis instrument of claim 1 , wherein the analysis instrument is scalable.

14. The analysis instrument of claim 1 , wherein the plurality of modules comprises two or more modules.

15. The analysis instrument of claim 1 , wherein the biochemical analysis is sequencing of polynucleotides.

16. The analysis instrument of claim 15 , wherein the control system is configured to direct the sequencing of a defined number of polynucleotides in each flow cell.

17. The analysis instrument of claim 15 , wherein the sequencing comprises an imaging stage to detect the incorporation of chemically labelled fluorescent probes.

18. The analysis instrument of claim 15 , wherein the sequencing comprises an imaging stage to detect the annealing of chemically labelled fluorescent probes.

19. The analysis instrument of claim 15 , wherein the sequencing comprises an imaging stage to detect the removal of chemically labelled fluorescent probes.

20. The analysis instrument of claim 1 , wherein each module can alternatively be operated as an independent unit for performing biochemical analysis.

21. The analysis instrument of claim 1 , wherein the global performance targets are set by user input or stored data corresponding to the biochemical analysis being performed.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded May 15, 2019
From: BROWN, CLIVE GAVIN; WILLCOCKS, JAMES PETER
To: OXFORD NANOPORE TECHNOLOGIES LTD.
Reel/Frame 049184/0827 →
Priority Claims (2)
GB 0922743.0 · Dec 31, 2009 · national
GB 1016614.8 · Oct 1, 2010 · national
Continuity (5)
Continuation 15491450 · Apr 19, 2017
Continuation 14302303 · Jun 11, 2014
Continuation 13512937
Provisional Application 61265488 · Dec 1, 2009
Related Publication 20190204267A1 · Jul 4, 2019
Cited By (2)
US 12,247,946 US 12,553,860