Sample processing improvements for microscopy
Among other things, a first surface is configured to receive a sample and is to be used in a microscopy device. There is a second surface to be moved into a predefined position relative to the first surface to form a sample space that is between the first surface and the second surface and contains at least part of the sample. There is a mechanism configured to move the second surface from an initial position into the predefined position to form the sample space. When the sample is in place on the first surface, the motion of the second surface includes a trajectory that is not solely a linear motion of the second surface towards the first surface.
1. An apparatus comprising
a two-dimensional arrangement of light sensitive elements exposed at a sensor surface of an imaging sensor, the imaging sensor to bear a sample for near-field microscopy, and
a mixing mechanism configured to cause mixing of the sample at the sensor surface, the mixing mechanism being configured (a) to cause a second surface to be moved into a predefined position relative to the sensor surface to form a sample space that is between the sensor surface and the second surface and contains at least part of the sample and (b) to move the second surface toward and away from the sensor surface before the sensor surface is moved into the predefined position.
2. The apparatus of claim 1 in which the mixing of the sample comprises causing units of the sample to be evenly distributed in the sample.
3. The apparatus of claim 1 in which the mechanism comprises a controller to control the mixing automatically.
4. The apparatus of claim 1 in which the sample comprises elements to be counted, and the mechanism comprises a controller to cause the elements to be evenly distributed across the sensor surface.
5. The apparatus of claim 1 comprising a controller to control the mixing to cause a bulk concentration of the elements in the sample after the second surface reaches the predefined position to be consistently proportional to a bulk concentration of the elements in the sample when the second surface is in an initial position prior to the mixing.
6. The apparatus of claim 1 comprising a controller to control the mechanism to cause the bulk concentration of elements in the sample after the second surface reaches the predefined position to be the same as or higher than the bulk concentration of the elements in the sample when the second surface is in the initial position.
7. The apparatus of claim 1 in which the mechanism configured to cause mixing comprises
a wall;
a second surface comprising a side having a width and a contact edge along the side, the contact edge bearing against the wall, the second surface being configured to be rotatable about the contact edge to form a sample space between the sensor surface and the second surface; and
the contact edge having a discontinuity along the side of the second surface.
8. An apparatus, comprising:
a light-sensitive sensor having a sensor surface to bear a sample for near-field microscopy;
a wall;
a second surface comprising a side having a width and a contact edge along the side, the contact edge bearing against the wall, the second surface being configured to be rotatable about the contact edge to form a sample space between the sensor surface and the second surface; and
the contact edge having a discontinuity along the side of the second surface.
9. The apparatus of claim 8 in which the contact edge comprises two extending points of the second surface.
10. The apparatus of claim 8 comprising a mechanism configured to rotate the second surface about the contact edge.
11. The apparatus of claim 10 in which the mechanism comprises a controller to control the mixing automatically.
12. The apparatus of claim 10 in which the sample comprises elements to be counted, and the mechanism comprises a controller to cause the elements to be evenly distributed across the sensor surface.
13. The apparatus of claim 10 comprising a controller to control the mixing to cause the bulk concentration of elements in the sample after the sample space has been formed to be consistently proportional to a bulk concentration of the elements in the sample before the sample space has been formed.
14. The apparatus of claim 10 comprising a controller to control the mixing to cause the bulk concentration of elements in the sample after the sample space has been formed to be the same as or higher than the bulk concentration of the elements in the sample before the sample space has been formed.