Granted Patent B2

US 12,388,957 · App. 18/662,064 · Granted Aug 12, 2025

Inventors: Alan Marc Fine (Prospect, CA); Hershel Macaulay (Cambridge, MA); Laurent Kreplak (Halifax, CA)

Assignee: Alentic Microscience Inc.

H04N7/18G02B21/0008B01L3/502761B01L2200/027

Abstract

Claims (39)

1. A microscope apparatus comprising:

a surface configured to receive a whole blood sample of a person or animal, the whole blood sample comprising blood cells of two or more types;

a two-dimensional array of light-sensitive elements that are configured and arranged to be sensitive to light at the surface;

a movable lid that defines a top of a sample space in which the whole blood sample is arranged on the surface, wherein the movable lid comprises projections extending from a lower surface of the movable lid, the projections defining a height of the sample space; and

a device comprising a memory and one or more microprocessors, wherein the device is configured to:

capture at least one image of the whole blood sample using the two-dimensional array of light-sensitive elements, and

generate a blood count of blood cells in the whole blood sample using the at least one image.

2. The microscope apparatus of claim 1 , wherein the lid is configured to define a precisely-specified volume of the whole blood sample at the surface.

3. The microscope apparatus of claim 1 , wherein the projections comprise molded polymeric projections.

4. The microscope apparatus of claim 1 , comprising beads of a uniform size within the whole blood sample or attached to the lower surface of the lid, the beads defining the height of the sample space.

5. The microscope apparatus of claim 1 , wherein the device is configured to generate the blood count based on different respective cytoplasm colors of blood cells of each of two or more types in the whole blood sample.

6. The microscope apparatus of claim 1 , wherein the device is configured to generate the blood count based on whether blood cells of each of two or more types in the whole blood sample include a nucleus.

7. The microscope apparatus of claim 1 , wherein the projections are configured such that the height of the sample space is in a range from 10 μm to 100 μm.

8. The microscope apparatus of claim 1 , wherein the surface is within a near-field distance of the two-dimensional array of light-sensitive elements.

9. The microscope apparatus of claim 1 , wherein the surface comprises a coating comprising a molecular layer configured to enhance changes in a shape of the blood cells.

10. The microscope apparatus of claim 1 , wherein the projections are configured such that the whole blood sample has a microliter-scale volume.

11. The microscope apparatus of claim 1 , wherein the device is configured to derive morphometric information about the blood cells at a resolution of 5 μm or less based on the at least one image, wherein the morphometric information includes a shape of the blood cells of each of the two or more types,

wherein generating the blood count comprises differentiating between blood cells of each of two or more types based on the shape of the blood cells of each of the two or more types.

12. The microscope apparatus of claim 1 , wherein the projections comprise a silicone polymer.

13. The microscope apparatus of claim 1 , wherein the projections are configured to contact the surface configured to receive the whole blood sample.

14. The microscope apparatus of claim 1 , wherein the projections are molded in the lower surface of the lid.

15. The microscope apparatus of claim 1 , wherein the projections are included in a layer attached to the lower surface of the lid.

16. The microscope apparatus of claim 15 , wherein the projections are embossed or cast.

17. A blood count method comprising:

arranging a whole blood sample of a person or animal on a surface;

forming a sample space for the whole blood sample using a movable lid that defines a top of the sample space, wherein the movable lid comprises projections extending from a lower surface of the movable lid, the projections defining a height of the sample space;

capturing at least one image of the whole blood sample using a two-dimensional array of light-sensitive elements that are configured and arranged to be sensitive to light at the surface; and

generating a blood count of blood cells in the whole blood sample using the at least one image.

18. The blood count method of claim 17 , comprising forming a precisely-specified volume of the whole blood sample at the surface based on the height of the sample space.

19. The blood count method of claim 17 , comprising generating the blood count based on at least one of:

different respective cytoplasm colors of blood cells of each of two or more types in the whole blood sample, or

whether the blood cells of the each of the two or more types include a nucleus.

20. The blood count method of claim 17 , comprising capturing the at least one image while the surface is within a near-field distance of the two-dimensional array of light-sensitive elements.

21. The blood count method of claim 17 , comprising setting a height of the whole blood sample to be between 10 82 m and 100 μm.

22. The blood count method of claim 17 , wherein the whole blood sample has a microliter-scale volume.

23. The blood count method of claim 17 , comprising inspecting the whole blood sample using a scanning probe microscope based on information derived from the at least one image.

24. The blood count method of claim 17 , comprising:

based on the at least one image, deriving morphometric information about the blood cells at a resolution of 5 μm or less, wherein the morphometric information includes a shape of blood cells of each of two or more types in the whole blood sample,

wherein generating the blood count comprises differentiating between blood cells of each of two or more types based on the shape of the blood cells of each of the two or more types.

Assignments (2)

Continuity (5)

References Cited (400)