Reduction of erythrocyte sedimentation rate
The present invention relates to reduction of erythrocyte sedimentation rate in a blood sample. In particular, formulations, compositions, articles of manufacture, kits and methods for reduced erythrocyte sedimentation rate in a blood sample are provided.
1. An in vitro method for reducing the erythrocyte sedimentation rate in a blood sample, comprising: combining a sample of blood with an amount of a formulation comprising sucralose sufficient to produce a treated blood sample having a sucralose concentration of about 5 mM to about 50 mM sucralose, thereby reducing the erythrocyte sedimentation rate as compared to erythrocyte sedimentation rate in an untreated blood sample.
2. The method of claim 1 , wherein the treated blood sample has a sucralose concentration of about 5 mM up to 25 mM sucralose.
3. The method of any one of claim 1 , wherein erythrocyte sedimentation rate is reduced by at least 10%, at least 20%, at least 30%, at least 40%, at least 50%, at least 60%, at least 70%, at least 80%, at least 90%, at least 95% of the erythrocyte sedimentation rate of the untreated blood sample.
4. The method of claim 1 , wherein the formulation is in the form of a powder, a solid, a lyophilized form, a solution, or an aqueous solution.
5. The method of any one of claim 1 , wherein the formulation consists of sucralose.
6. The method of claim 1 , wherein the formulation further comprises an anticoagulant.
7. The method of claim 6 , wherein the anticoagulant is selected from the group consisting of acid citrate dextrose solution (ACD), sodium heparin, sodium fluoride, lithium heparin, tri-potassium ethylenediaminetetraacetic acid (K3EDTA), di-potassium ethylenediaminetetraacetic acid (K2EDTA), hirudin, and sodium polyanethol sulfonate (SPS).
8. The method of any one of claim 1 , wherein the formulation is contained within a blood collection tube, and the combining step occurs within the blood collection tube.
9. The method of claim 8 , wherein the blood collection tube is an evacuated blood collection tube.
10. The method of claim 1 , wherein one or more erythrocytes of the treated blood sample are maintained in suspension for a period of at least 30 minutes as compared to an untreated blood sample.
11. A composition comprising a blood sample and sucralose, wherein the sucralose is at a concentration of about 5 mM sucralose up to but not including 50 mM sucralose.
12. The composition of claim 11 , wherein the sucralose is at a concentration of about 5 mM sucralose up to but not including 25 mM sucralose.
13. The composition of claim 11 , further comprising an anticoagulant.
14. The composition of claim 13 , wherein the anticoagulant is selected from the group consisting of acid citrate dextrose solution (ACD), sodium heparin, sodium fluoride, lithium heparin, tri-potassium ethylenediaminetetraacetic acid (K3EDTA), di-potassium ethylenediaminetetraacetic acid (K2EDTA), hirudin, and sodium polyanethol sulfonate (SPS).
15. The composition of claim 11 , wherein the blood is human.