Liquid laundry detergent composition
The present invention relates to a liquid detergent composition comprising a novel protease, more in particular a novel and subtilase variant with increased stability, a method for (dish) washing using said liquid detergent composition and a use of said liquid composition in a (dish) washing process.
1. A liquid detergent composition comprising a protease having the amino acid sequence of SEQ ID NO: 1, wherein the protease is a subtilase variant comprising the substitutions X9E+X206L+X262E.
2. A liquid detergent composition according to claim 1 , wherein the protease further comprises the substitution X76D.
3. A liquid detergent composition according to claim 1 , wherein the protease further comprises one or more alterations selected from the group consisting of X3T, X4I, X14T, X24G, X24R, X27R, *36D, X43A, X43C, X43L, X43R, X43W, X68A, X72A, X72V, X78D, X87R, X87S, *97E, X98S, X99A, X99D, X99A, X99D, X99E, X99G, *99D, X101D, X101E, X101G, X101I, X101K, X101L, X101M, X101N, X101R, X103A, X104F, X104I, X104N, X104Y, X106A, X114V, X115T, X115W, X118R, X118V, X120D, X120I, X120N, X120T, X120V, X123S, X128A, X128L, X128S, X129D, X129N, X129Q, X130A, X147W, X149C, X149N, X158E, X160D, X160P, X161C, X161E, X162L, X163A, X163D, X167A, X170S, X182C, X182E, X185C, X185E, X188C, X188D, X188E, X191N, X194P, X195E, X199M, X204D, X204V, X205I, X209W, X212A, X212D, X212G, X212N, X216I, X216T, X216V, X217C, X217D, X217E, X217M, X217Q, X217Y, X218D, X218E, X218T, X222C, X222R, X222S, X225A, X232V, X235L, X236H, X245K, X245R, X252K, X255C, X255E, X256A, X256C, X256D, X256V, X256Y, X259D, X260E, X260P, X261C, X261E, X261F, X261L, X261M, X261V, X261W, X261Y and X274A.
4. A liquid detergent composition according to claim 1 , wherein the protease further comprises 1, 2, 3, 4, 5, 6, or all of the substitutions selected from the group consisting of : X43R, X185E, X188E, X191N X194P, X209W, X259D.
5. A liquid detergent composition according to claim 1 , wherein the protease is a variant of a subtilase originating from Bacillus lentus.
6. A liquid detergent composition according to claim 1 , which comprises one or more additional enzymes selected from the group consisting of amylases, catalases, cellulases, cutinases, haloperoxygenases, lipases, mannanases, pectinases, pectin lyases, peroxidases, proteases, xanthanases, and xyloglucanases, or any mixture thereof.
7. A liquid detergent composition according to claim 1 , which comprises peptide based protease inhibitor, wherein the peptide based protease inhibitor has the following structure: peptide compounds of the formula B 2 -B 1 -B 0 -R, wherein
R is hydrogen, CH 3 , CX 3 , CHX 2 or CH 2 X, wherein X is a halogen atom;
B 0 is phenylalanine residue with an OH substituent at the p-position and/or at the m-position;
B 1 is a single amino acid residue; and
B 2 consists of one or more amino acid residues, optionally comprising an N-terminal protection group.
8. A liquid detergent composition according to claim 1 , wherein the detergent composition is packaged in the form of a unit-dosed packaged liquid detergent composition.
9. A liquid detergent composition according to claim 1 , wherein the total amount of surfactant is from 0.1 to 85% w/w.
10. A liquid detergent composition according to claim 1 , wherein the liquid detergent composition is a liquid dish wash composition or a liquid laundry composition.
11. A method of laundering using a liquid laundry detergent composition comprising a protease having the amino acid sequence of SEQ ID NO: 1, wherein the protease is a subtilase variant comprising the substitutions X9E+X206L+X262E, comprising the step of contacting the fabric with the liquid laundry detergent composition under conditions suitable for cleaning the fabric.