Automatic dishwashing composition comprising a protease
Fabric and home care compositions including a surfactant and a protease. The automatic dishwashing composition is in the form of a water-soluble unit-dose pouch including a compartment. The compartment includes both a powder component and a gel component. The protease is a subtilisin variant that includes: (i) a mutation at position 122; and (ii) one or more mutations at positions 126, 127, 128, 211 and 212, with reference to SEQ ID NO: 1. The variant has at least about 80% identity to the amino acid sequence of SEQ ID NO: 1.
1 . An automatic dishwashing composition comprising a surfactant and a protease,
wherein the automatic dishwashing composition is in the form of a water-soluble unit-dose pouch comprising a compartment, wherein the compartment comprises both a powder component and a gel component, and
wherein the protease comprises a subtilisin variant that comprises:
(i) a mutation at position 122; and
(ii) one or more mutations at positions 126, 127, 128, 211 and 212,
with reference to SEQ ID NO: 1,
wherein the subtilisin variant has 88% or more identity to the amino acid sequence of SEQ ID NO: 1.
2 . The composition according to claim 1 , wherein the composition is in the form of a multi-compartment pouch that comprises two or more compartments.
3 . The composition according to claim 1 , wherein the protease is present in the gel component.
4 . The composition according to claim 1 , further comprising a bleach, wherein the bleach is present in the powder component.
5 . The composition according to claim 1 , wherein the subtilisin variant comprises:
(i) the mutation at position M122L; and
(ii) one or more mutations at positions 126, 127, 128, 211 and 212.
6 . The composition according to claim 1 , wherein the subtilisin variant comprises two or more mutations at positions 126, 127, 128, 211 and 212.
7 . The composition according to claim 1 , wherein the subtilisin variant comprises one or more mutations selected from S126A, D127E, F128G, M211Q and N212Q.
8 . The composition according to claim 1 , wherein the subtilisin variant comprises the mutations M122L, S126A, D127E, F128G, M211Q and N212Q.
9 . The composition according to claim 1 , wherein the subtilisin variant comprises an amino acid sequence having 89% sequence identity to the amino acid sequence of SEQ ID NO: 1.
10 . The composition according to claim 1 , wherein the subtilisin variant is derived from a parent or reference polypeptide with at least 60% amino acid sequence identity to SEQ ID NO: 1.
11 . The composition according to claim 1 , wherein the composition further comprises a manganese bleach catalyst selected from the group consisting of 1,4,7-trimethyl-1,4,7-triazacyclononane (Me-TACN), 1,2, 4,7-tetramethyl-1,4,7-triazacyclononane (Me/Me-TACN), and mixtures thereof.
12 . The composition according to claim 1 , wherein the composition further comprises one or more other enzymes selected from acyl transferases, amylases, alpha-amylases, beta-amylases, alpha-galactosidases, arabinases, arabinosidases, aryl esterases, beta-galactosidases, beta-glucanases, carrageenases, catalases, cellulases, chondroitinases, cutinases, dispersins, endo-glucanases, endo-beta-mannanases, exo-beta-mannanases, esterases, exo-mannanases, galactanases, glucoamylases, hemicellulases, hexosaminidase, hyaluronidases, keratinases, laccases, lactases, ligninases, lipases, lipolytic enzymes, lipoxygenases, lysozyme, metalloproteases, mannanases, nucleases, oxidases, oxidoreductases, pectate lyases, pectin acetyl esterases, pectinases, pentosanases, perhydrolases, peroxidases, PETases, phenoloxidases, phosphatases, phospholipases, phytases, polyesterases, polygalacturonases, additional proteases, pullulanases, reductases, rhamnogalacturonases, tannases, transglutaminases, xylan acetyl-esterases, xylanases, and xylosidases; and combinations thereof.
13 . The composition according to claim 12 , wherein the one or more enzymes comprises an amylase selected from the group consisting of AA707, AA560, AAI10, SP722, BspAmy24, and CspAmy1, variants thereof, and combinations thereof.
14 . The composition according to claim 12 , wherein the one or more enzymes comprises a recombinant, non-naturally-occurring variant of a parent alpha-amylase.
15 . The composition according to claim 1 , wherein the subtilisin variant comprises an amino acid sequence having 89% or more sequence identity to the amino acid sequence of SEQ ID NO: 1.
16 . The composition according to claim 1 , wherein the subtilisin variant comprises an amino acid sequence having 90% sequence identity to the amino acid sequence of SEQ ID NO: 1.
17 . The composition according to claim 1 , wherein the subtilisin variant comprises an amino acid sequence having 90% or more sequence identity to the amino acid sequence of SEQ ID NO: 1.