Process for enzymatic hydrolysis of lignocellulosic material and fermentation of sugars
The invention relates to a process for the preparation of a fermentation product from ligno-cellulosic material, comprising the following steps: a) optionally pre-treatment of the ligno-cellulosic material; b) optionally washing of the optionally pre-treated ligno-cellulosic material; c) enzymatic hydrolysis of the optionally washed and/or optionally pre-treated ligno-cellulosic material using an enzyme composition comprising at least two cellulase and whereby the enzyme composition at least comprises GH61; d) fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product; and e) optionally recovery of a fermentation product; wherein before and/or during the enzymatic hydrolysis oxygen is added to the ligno-cellulosic material.
1. A process for preparing a sugar product from ligno-cellulosic material, comprising:
enzymatic hydrolysis, in a reactor, of the ligno-cellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises GH61;
wherein
oxygen is added continuously or discontinuously to the ligno-cellulosic material during the first half in time of the enzymatic hydrolysis;
the reactor has a volume of 1 m 3 or more; and
the ligno-cellulosic material has a dry matter content of 10 wt % or more.
2. The process of claim 1 , further comprising fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product.
3. The process according to claim 1 , wherein the oxygen is added in the form of bubbles.
4. The process according to claim 2 , wherein the enzymatic hydrolysis time is 5 to 150 hours.
5. The process according to claim 1 , wherein the enzyme composition used retains activity for 30 hours or more.
6. The process according to claim 1 , wherein the enzymatic hydrolysis is conducted at a temperature of 45° C. or more.
7. The process according to claim 1 , wherein the enzyme composition is from a fungus or the enzyme composition comprises a fungal enzyme.
8. The process according to claim 1 , wherein the dry matter content is 14 to 33% wt %.
9. The process according to claim 1 in which the enzymatic hydrolysis takes place in a batch, fed batch and/or continuous culture reactor.
10. The process according to claim 1 in which oxygen is introduced as an oxygen-containing gas, optionally air.
11. The process according to claim 7 , wherein the enzyme composition is from a fungus, and the fungus is a microorganism of the genus Rasamsonia.
12. The process according to claim 7 , wherein the enzyme composition comprises a fungal enzyme, and the fungal enzyme is a Rasamsonia enzyme.
13. The process of claim 1 , further comprising pre-treatment of the ligno-cellulosic material, prior to the enzymatic hydrolysis.
14. The process of claim 1 , further comprising washing of the ligno-cellulosic material, prior to the enzymatic hydrolysis.
15. The process of claim 1 , further comprising recovery of a sugar product from the enzymatic hydrolysis.
16. The process of claim 1 , further comprising, prior to the enzymatic hydrolysis, pre-treatment of the ligno-cellulosic material and washing of the pre-treated ligno-cellulosic material.
17. The process of claim 1 , further comprising pre-treatment of the ligno-cellulosic material prior to the enzymatic hydrolysis and recovery of a sugar product from the enzymatic hydrolysis.
18. The process of claim 1 , further comprising pre-treatment of the ligno-cellulosic material and washing of the pre-treated ligno-cellulosic material, prior to the enzymatic hydrolysis, and recovery of a sugar product from the enzymatic hydrolysis.
19. The process of claim 1 , wherein the oxygen is added continuously.
20. The process of claim 1 , wherein the oxygen is added discontinuously.
21. The process of claim 2 , wherein the fermentation product is ethanol.
22. A process for preparing a sugar product from ligno-cellulosic material, comprising:
enzymatic hydrolysis, in a reactor, of the ligno-cellulosic material using an enzyme composition comprising at least two cellulases and whereby the enzyme composition at least comprises GH61;
wherein
oxygen is added continuously or discontinuously to the ligno-cellulosic material during the second half in time of the enzymatic hydrolysis;
the reactor has a volume of 1 m 3 or more; and
the ligno-cellulosic material has a dry matter content of 10 wt % or more.
23. The process of claim 22 , further comprising fermentation of the hydrolysed ligno-cellulosic material to produce a fermentation product.
24. The process according to claim 22 , wherein the oxygen is added in the form of bubbles.
25. The process according to claim 23 , wherein the enzymatic hydrolysis time is 5 to 150 hours.
26. The process according to claim 22 , wherein the enzyme composition used retains activity for 30 hours or more.
27. The process according to claim 22 , wherein the enzymatic hydrolysis is conducted at a temperature of 45° C. or more.
28. The process according to claim 22 , wherein the enzyme composition is from a fungus or the enzyme composition comprises a fungal enzyme.
29. The process according to claim 22 , wherein the dry matter content is 14 to 33% wt %.
30. The process according to claim 22 in which the enzymatic hydrolysis takes place in a batch, fed batch and/or continuous culture reactor.
31. The process according to claim 22 in which oxygen is introduced as an oxygen-containing gas, optionally air.
32. The process according to claim 28 , wherein the enzyme composition is from a fungus, and the fungus is a microorganism of the genus Rasamsonia.
33. The process according to claim 28 , wherein the enzyme composition comprises a fungal enzyme, and the fungal enzyme is a Rasamsonia enzyme.
34. The process of claim 22 , further comprising pre-treatment of the ligno-cellulosic material, prior to the enzymatic hydrolysis.
35. The process of claim 22 , further comprising washing of the ligno-cellulosic material, prior to the enzymatic hydrolysis.
36. The process of claim 22 , further comprising recovery of a sugar product from the enzymatic hydrolysis.
37. The process of claim 22 , further comprising, prior to the enzymatic hydrolysis, pre-treatment of the ligno-cellulosic material and washing of the pre-treated ligno-cellulosic material.
38. The process of claim 22 , further comprising pre-treatment of the ligno-cellulosic material prior to the enzymatic hydrolysis and recovery of a sugar product from the enzymatic hydrolysis.
39. The process of claim 22 , further comprising pre-treatment of the ligno-cellulosic material and washing of the pre-treated ligno-cellulosic material, prior to the enzymatic hydrolysis, and recovery of a sugar product from the enzymatic hydrolysis.
40. The process of claim 22 , wherein the oxygen is added continuously.
41. The process of claim 22 , wherein the oxygen is added discontinuously.
42. The process of claim 23 , wherein the fermentation product is ethanol.