Lincosamides universal monoclonal antibody hybridoma cell strain and application thereof
The present disclosure discloses a lincosamides universal monoclonal antibody hybridoma cell strain and application thereof, and belongs to the technical field of food safety immunological detection. According to the present disclosure, a clindamycin chlorine-substituted derivative is used as a hapten, the hapten is coupled with bovine serum albumin (BSA) by an activated ester method to obtain an immunizing antigen, and after being uniformly mixed with a Freund's adjuvant, the immunizing antigen is subcutaneously injected to immunize BALB/c mice; clindamycin is coupled with ovalbumin (OVA) by a carbonyl diimidazole (CDI) method to be used as a coating antigen used for detecting mouse serums and a cell supernatant. The spleen cells of the immunized mice are fused with mouse myeloma cells by a PEG method, and screened by indirect ELISA and indirect competitive ELISA and subcloned three times to obtain a population-selective hybridoma cell strain. The cell strain provided by the present disclosure has relatively good inhibition on clindamycin, lincomycin and pirlimycin, and can meet the demand for lincosamides multi-residue immunoassay products on the market.
1. A monoclonal cell strain, preserved at the China General Microbiological Culture Collection Center on Sep. 5, 2017, wherein the preservation number is CGMCC No. 14691, and the preservation address is No. 3, No. 1 Courtyard, Beichen West Road, Chaoyang District, Beijing, China.
2. A lincosamides-specific monoclonal antibody, wherein the lincosamide-specific monoclonal antibody is produced by secretion of the monoclonal cell strain according to claim 1 .
3. A method for using the lincosamides-specific monoclonal antibody according to claim 2 , comprising analyzing and detecting lincosamides residues using the lincosamides-specific monoclonal in food safety detection.
4. The method according to claim 3 , further comprising detecting a lincosamides drug by enzyme-linked immunosorbent assay using the lincosamides-specific monoclonal antibody.
5. The method according to claim 4 wherein the lincosamides drug comprises clindamycin, lincomycin or pirlimycin.
6. The method according to claim 4 , wherein the lincosamides drug comprises clindamycin, lincomycin and pirlimycin.
7. The method according to claim 3 , wherein the lincosamides-specific monoclonal antibody is applied to a detection kit.
8. The method according to claim 7 , comprising using the kit for detecting the lincosamides drug.
9. The method according to claim 8 , wherein the lincosamide drug comprises clindamycin, lincomycin or pirlimycin.
10. A kit comprising the lincosamides-specific monoclonal antibody according to claim 2 .