Anti-tetrodotoxin antibody K548, and preparation method and use thereof
The present disclosure relates to the technical field of biomedicine, and provides an anti-tetrodotoxin humanized antibody and use thereof. The humanized antibody has a heavy-chain variable region and a light-chain variable region with amino acid sequences shown in SEQ ID NO: 1 to SEQ ID NO: 2, respectively. Affinity analysis shows that the antibody of the present disclosure has prominent affinity. It is proved by experiments that, after mice in an antibody protection group pre-injected with the antibody of the present disclosure are injected with tetrodotoxin, no mice shows toxic symptoms, and during continuous observation for one month, no toxic lethality occurs, indicating that the antibody of the present disclosure shows excellent anti-tetrodotoxin effects, excellent preventive or therapeutic effects on puffer fish-related biological injuries, and promising clinical application prospects.
1 . An anti-tetrodotoxin humanized antibody K548, comprising a heavy-chain variable region with an amino acid sequence shown in SEQ ID NO: 1, and a light-chain variable region with an amino acid sequence shown in SEQ ID NO: 2.
2 . The anti-tetrodotoxin antibody K548 according to claim 1 , wherein the heavy-chain variable region comprises FRH1-CDRH1-FRH2-CDRH2-FRH3-CDRH3-FRH4 regions; and the light-chain variable region comprises FRL1-CDRL1-FRL2-CDRL2-FRL3-CDRL3-FRL4 regions.
3 . A nucleotide encoding the anti-tetrodotoxin antibody K548 according to claim 1 .
4 . A preparation method of the anti-tetrodotoxin antibody K548 according to claim 1 , comprising the following steps: (A) synthesizing a polynucleotide encoding a full-length humanized antibody based on a whole genome of a variable region gene of the antibody K548; (B) cloning the polynucleotide obtained in step (A) into an expression vector by PCR, and determining a correct clone by sequencing verification; and (C) introducing the expression vector into a host cell for fusion protein expression.
5 . The preparation method according to claim 4 , wherein
the expression vector is pGEM-T, Pet32a, pcDNA3.1, pEE6.4, pEE12.4, pDHFR, or pDR1; the expression vector comprises a fusion DNA sequence ligated with appropriate transcription and translation regulatory sequences; and
the host cell is a prokaryotic cell, a mammalian cell, a bacterial cell, an insect cell, or a fungal cell.
6 . A pharmaceutical composition comprising the anti-tetrodotoxin antibody K548 according to claim 1 , wherein the pharmaceutical composition further comprises a pharmaceutically acceptable drug carrier.
7 . The pharmaceutical composition according to claim 6 , wherein
the pharmaceutical composition is an injection or a lyophilized formulation; and
the pharmaceutically acceptable drug carrier comprises one or a combination of two or more from the group consisting of a surfactant, a solution stabilizer, an isoosmotic adjusting agent, and a buffer.
8 . The pharmaceutical composition according to claim 6 , wherein
the injection or the lyophilized formulation is intravenously injected at a dosage of 1 mg/d to 1,800 mg/d.