Method of distinguishing constipation predominant IBS from inflammatory bowel syndrome by analysis of methane concentration
Disclosed are methods of diagnosing constipation and constipation-predominant irritable bowel syndrome by screening for the presence of abnormally high levels of methane and/or methanogenic organisms in a subject.
1. A method of analyzing a level of methane in a breath sample from a subject and diagnosing the subject as having constipation-predominant IBS (C-IBS) and not inflammatory bowel disease (IBD), comprising:
obtaining a breath sample from the subject;
analyzing the breath sample comprising measuring the level of methane in the breath sample, determining an abnormally elevated level of methane in the breath sample as compared to a healthy individual comprising detecting the methane level by conducting a lactulose breath test (LBT) or by gas chromatography; and
diagnosing the subject as having C-IBS and not IBD when the methane level in the breath sample from the subject is greater than the methane level from said healthy individual, wherein the methane is from methane producing organisms in the intestines of the subject.
2. The method of claim 1 , wherein the methane is detected by conducting a lactulose breath test (LBT).
3. The method of claim 2 , further comprising: having the subject swallow lactulose after an initial base line breath sample; and collecting breath samples at intervals.
4. The method of claim 1 , wherein methane is detected by gas chromatography.