Bead-based analysis of a sample
View Patent ↗A method includes attaching two or more beads to each unit of one or more units of a chemical component in a sample, to form, for each unit of the chemical component, a multi-bead complex including two or more beads and the unit of the chemical component; placing the sample on a surface of an image sensor; at the image sensor, receiving light originating at a light source, the received light including light reflected by, refracted by, or transmitted through the beads of the multi-bead complexes; at the image sensor, capturing one or more images of the sample from the received light; and identifying, in at least one of the images of the sample, separate multi-bead complexes, the identifying of the separate multi-bead complexes including associating the two or more beads of each of the multi-bead complexes based on proximity to one another.
1. A method for identifying multi-bead complexes comprising:
placing, on a surface of an image sensor, a sample comprising one or more units of a chemical component, each unit of the chemical component being included in a corresponding multi-bead complex comprising two or more beads and the unit of the chemical component, the two or more beads being attached to the unit of the chemical component,
at the image sensor, receiving light originating at a light source, the received light including light reflected by, refracted by, or transmitted through the two or more beads of each multi-bead complex,
at the image sensor, capturing one or more images of the sample from the received light,
measuring, in at least one of the images of the sample, a proximity to one another of the two or more beads of at least two different multi-bead complexes, and
identifying, in the at least one of the images of the sample, two or more separate multi-bead complexes based on the measured proximity to one another of the two or more beads of the at least two different multi-bead complexes.
2. The method of claim 1 , comprising identifying a presence of the chemical component based on the identification of the two or more separate multi-bead complexes.
3. The method of claim 1 , comprising identifying a level of the chemical component based on the identification of the two or more separate multi-bead complexes.
4. The method of claim 1 , in which identifying the two or more separate multi-bead complexes comprises enumerating the two or more separate multi-bead complexes.
5. The method of claim 1 , in which the sample comprises attachment units, and in which, for each multi-bead complex comprising two or more beads and a unit of the chemical component,
two or more attachment units are bound to the unit of the chemical component of the multi-bead complex, and
each attachment unit of the two or more attachment units of the multi-bead complex is bound to one or more beads of the two or more beads of the multi-bead complex, such that each multi-bead complex comprises two or more beads, two or more attachment units, and the unit of the chemical component.
6. The method of claim 5 , in which the attachment units comprise antibodies.
7. The method of claim 5 , in which the attachment units comprise antigens from a pathogen, and in which the one or more units of the chemical component comprise antibodies to the pathogen.
8. The method of claim 7 , in which the pathogen comprises a virus.
9. The method of claim 5 , in which at least two attachment units of the two or more attachment units of each multi-bead complex are different from one another.
10. The method of claim 5 , in which the two or more attachment units of each multi-bead complex bind at different locations at the unit of the chemical component of the multi-bead complex.
11. The method of claim 1 , in which at least two beads of the two or more beads of a first multi-bead complex of the multi-bead complexes have the same reflective, refractive, and transmissive characteristics.
12. The method of claim 1 , in which at least two beads of the two or more beads of a first multi-bead complex of the multi-bead complexes differ in at least one of appearance, reflective characteristics, refractive characteristics, or transmissive characteristics, for the light originating at the light source.
13. The method of claim 12 , in which the appearance, reflective characteristics, refractive characteristics, or transmissive characteristics comprise at least one of colors of the at least two beads, sizes of the at least two beads, shapes of the at least two beads, or birefringence of the at least two beads.
14. The method of claim 1 , in which the one or more units of the chemical component comprise an antibody of a pathogenic virus.
15. The method of claim 1 , in which placing the sample on the surface of the image sensor comprises forming a monolayer of the sample on the surface of the image sensor.
16. The method of claim 1 , in which placing the sample on the surface of the image sensor comprises confining the sample between the surface of the image sensor and a second surface opposite the surface of the image sensor.
17. The method of claim 1 , comprising identifying, in at least one of the images of the sample, separate individual beads based on light reflected by, refracted by, or transmitted by each of the separate individual beads, and based on a proximity of each of the separate individual beads to other beads.