Vaccine delivery method
A vaccine delivery method is presented that includes a composition including as one component a slurry matrix that is a liquid at room temperature and a gel at physiological pH, physiological salt concentrations and/or physiological temperatures and as a second component one or more antigens. Also included are methods of inducing an immune response in a subject and vaccinating a subject by administering such compositions.
1. A vaccine composition comprising a peptide hydrogel of the peptide scaffold RADARADARADARADA (SEQ ID NO:2), an influenza antigen, and a TLR9 agonist, wherein the vaccine composition is a liquid at room temperature, non-physiological pH, and/or non-physiological salt concentrations and a gel at physiological pH, physiological salt concentrations and/or physiological temperatures, and wherein the vaccine composition gels after administration to a subject.
2. The vaccine composition of claim 1 , wherein the TLR9 agonist comprises a CpG oligodeoxynucleotide (ODN).
3. The vaccine composition of claim 1 , wherein the influenza antigen comprises an influenza A, influenza B, or influenza C antigen.
4. The vaccine composition of claim 1 , wherein the influenza antigen comprises a recombinant nucleoprotein (rNP) influenza virus antigen.
5. The vaccine composition of claim 1 , wherein the influenza antigen comprises a whole-inactivated influenza virus.
6. The vaccine composition of claim 1 , wherein the vaccine composition is lyopholized.
7. A method of producing an immune response in a subject, the method comprising administering a vaccine composition of claim 1 to the subject.
8. A method of vaccinating a subject to influenza, the method comprising administering a vaccine composition of claim 1 to the subject.
9. The method of claim 7 , wherein the subject is human.
10. The method of claim 7 , wherein administration of the composition comprises subcutaneous (sc) delivery, intramuscular (im) delivery, intradermal delivery, transdermal delivery, mucosal delivery, intravaginal delivery, intrarectal delivery, intraperitoneal delivery, inhalation delivery, and/or aerosol delivery.