Telomerase polypeptide vaccine for treating cancer
A polypeptide comprising the sequence of SEQ. ID NO. 2, 3, 4, 7 or 8. The polypeptide may have the sequence of an immunogenic fragment thereof comprising at least eight amino acids, wherein the immunogenic fragment is not one of SEQ. ID NOS. 6 or 11 to 16. The polypeptide may have a sequence having at least 80% sequence identity to the aforementioned polypeptide or immunogenic fragment. The polypeptide is less than 100 amino acids in length and does not comprise the sequence of any of SEQ. ID NOS. 10, 46, 56, 57 or 59 to 62 and does not consist of the sequence of SEQ ID NO. 58. The polypeptide is useful in the treatment or prophylaxis of cancer.
1. A method of eliciting a CD4+ and/or a CD8+ T-cell immune response in a patient with cancer or in an individual prior to any symptoms of cancer, comprising administering to the patient or the individual at least one selected from the group consisting of:
a) a polypeptide consisting of a sequence selected from the group consisting of:
SEQ ID NO: 1, or any one of SEQ ID NOS: 18, 24, 36 or 37; and
b) a pharmaceutical composition comprising a polypeptide of part a) and a pharmaceutically acceptable adjuvant, diluent or excipient.
2. The method according to claim 1 , wherein the cancer is selected from breast cancer, prostate cancer, pancreatic cancer, colorectal cancer, lung cancer, malignant melanoma, a leukemia, a lymphoma, ovarian cancer, cervical cancer, or biliary tract carcinoma.
3. The method according to claim 1 , part b), wherein the pharmaceutical composition comprises a further therapeutic ingredient.
4. The method according to claim 1 , part b), wherein the pharmaceutical composition comprises the polypeptide in a dose of between 1 and. 500 μg.
5. The method according to claim 1 , wherein the polypeptide is coupled to an immunogenic carrier.
6. The method according to claim 1 , wherein the method further comprises detecting the presence of the immune response in the patient or the individual.
7. The method according to claim 1 , wherein the polypeptide is linked to a further substance.
8. The method according to claim 7 , wherein the further substance is selected from the group consisting of lipids, sugar, sugar chains, acetyl groups, at least one further polypeptide, natural polymers and synthetic polymers.
9. The method according to claim 8 , wherein the polypeptide is in the form of a lipopeptide conjugate.
10. A method of eliciting a CD4+ and/or a CD8+ T- cell immune response in a patient with cancer or in an individual prior to any symptoms of cancer, comprising administering to the patient or the individual at least one selected from the group consisting of:
a) a cocktail of polypeptides comprising at least first and second different polypeptides wherein the first polypeptide consists of the sequence of
SEQ ID NO: 1;
and wherein the second polypeptide comprises a sequence selected from the group consisting of:
i) SEQ ID NOS: 2 to 10; and
ii) a fragment of i) comprising at least eight amino acids;
wherein the second polypeptide is less than or equal to 50 amino acids in length; and
b) a pharmaceutical composition comprising a cocktail of polypeptides of part a) and a pharmaceutically acceptable adjuvant, diluent or excipient.
11. The method according to claim 10 , wherein the at least first and second different polypeptides comprise a cocktail of polypeptides selected from the group consisting of:
i) a cocktail of: a polypeptide consisting of the sequence of SEQ ID NO: 1; a polypeptide comprising the sequence of SEQ ID NO: 8 or a fragment thereof comprising at least eight amino acids; and a polypeptide comprising the sequence of SEQ ID NO: 9 or a fragment thereof comprising at least eight amino acids; and
ii) a cocktail of: a polypeptide consisting of the sequence of SEQ ID NO: 1; a polypeptide comprising the sequence of SEQ ID NO: 8 or fragment thereof comprising at least eight amino acids; and a polypeptide comprising the sequence of SEQ ID NO: 10 or a fragment thereof comprising at least eight amino adds,
wherein each polypeptide is less than or equal to 50 amino acids in length.
12. The method according to claim 10 , wherein the cancer is selected from breast cancer, prostate cancer, pancreatic cancer, colorectal cancer, lung cancer, malignant melanoma, a leukemia, a. lymphoma, ovarian cancer, cervical cancer, or biliary tract carcinoma.
13. The method according to claim 10 , part b), wherein the pharmaceutical composition comprises a further therapeutic ingredient.
14. The method according to claim 10 , part h), wherein the pharmaceutical composition comprises the cocktail of polypeptides in a dose of between 1 and 500 μg.
15. The method according to claim 10 , wherein a polypeptide in the cocktail of polypeptides is coupled to an immunogenic carrier.
16. The method according to claim 10 , wherein the method further comprises detecting the presence of the immune response in the patient or the individual.
17. The method according claim 10 , wherein a polypeptide in the cocktail of polypeptides is linked to a further substance.
18. The method according to claim 17 , wherein the further substance is selected from the group consisting of lipids, sugar, sugar chains, acetyl groups, at least one further polypeptide, natural polymers and synthetic polymers.
19. The method according to claim 18 , wherein the polypeptide in the cocktail of polypeptides is in the form of a lipopeptide conjugate.
20. A method of eliciting a CD4+ and/or a CD8+ T-cell immune response in a patient with cancer or in an individual prior to any symptoms of cancer, comprising administering to the patient or the individual at least one selected from the group consisting of:
i) a cocktail of: a polypeptide consisting of the sequence of SEQ ID NO: 1, a polypeptide comprising the sequence of SEQ ID NO: 7 or a fragment thereof comprising at least eight amino acids; and a polypeptide comprising the sequence of SEO ID NO: 9 or a fragment thereof comprising at least eight amino acids;
ii) a cocktail of: a polypeptide comprising the sequence of SEQ ID NO: 1 or a fragment thereof comprising at least eight amino acids, a polypeptide consisting of the sequence of SEQ ID NO: 7; and a polypeptide comprising the sequence of SEQ ID NO: 9 or a fragment thereof comprising at least eight amino acids; and
iii) a cocktail of: a polypeptide comprising the sequence of SEQ ID NO: 1 or a fragment thereof comprising at least eight amino acids; a polypeptide comprising the sequence of SEQ ID NO: 7 or a fragment thereof comprising at least eight amino acids; and a polypeptide consisting of the sequence of SEQ ID NO: 9,
wherein each polypeptide is less than or equal to 50 amino acids in length.