Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics in response to cold
Methods and materials for modulating cold tolerance levels in plants are disclosed. For example, nucleic acids encoding cold tolerance-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased cold tolerance levels and plant products produced from plants having increased cold tolerance levels.
1. A method of producing a plant and/or plant tissue, said method comprising
growing plant cells comprising an exogenous nucleic acid sequence, said exogenous nucleic acid sequence comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide, wherein said polypeptide comprises an amino acid sequence that has 90% or greater sequence identity to the amino acid sequence of SEQ ID NO:20,
producing transformed plants from said transformed plant cells; and
selecting for a transformed plant from said transformed plants that has an increased level of cold tolerance as compared to the corresponding level of cold tolerance of a control plant that does not comprise said nucleic acid sequence, wherein the selected transformed plant expresses said polypeptide.
2. The method of claim 1 , wherein the polypeptide comprises an amino acid sequence that has 95% or greater sequence identity to the amino acid sequence of SEQ ID NO:20.
3. The method of claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO:20.
4. A method of increasing a level of cold tolerance in a plant, said method comprising
introducing into a plant an exogenous nucleic acid sequence, said exogenous nucleic acid sequence comprising a regulatory region operably linked to a nucleotide sequence encoding a polypeptide, wherein said polypeptide comprises an amino acid sequence that has 90% or greater sequence identity to the amino acid sequence of SEQ ID NO:20, and
selecting the plant for having an increased level of cold tolerance as compared to the corresponding level of cold tolerance of a control plant that does not comprise said nucleic acid sequence, wherein the plant expresses said polypeptide.
5. The method of increasing the level of cold tolerance in a plant according to claim 4 , wherein the polypeptide comprises an amino acid sequence that has 95% or greater sequence identity to the amino acid sequence of SEQ ID NO:20.