IP Library › Granted Patent US 12,735,717
Granted Patent B2
US 12,735,717 · App. 18/666,709 · Granted Sep 15, 2026

Nucleotide sequences and polypeptides encoded thereby useful for modifying plant characteristics in response to cold

Inventors: Cory Christensen (Zionsville, IN); Bonnie Hund (Pueblo, CO)
Assignee: CERES, INC.
C12N15/8273A01H1/00A01H1/12A01H1/1225A01H5/00
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,735,717
App. No.
18/666,709
Granted
Sep 15, 2026
Kind
B2
Abstract

Methods and materials for modulating cold tolerance levels in plants are disclosed. For example, nucleic acids encoding cold tolerance-modulating polypeptides are disclosed as well as methods for using such nucleic acids to transform plant cells. Also disclosed are plants having increased cold tolerance levels and plant products produced from plants having increased cold tolerance levels.

Claims (9)

1 . A plant cell comprising an exogenous nucleic acid, said exogenous nucleic acid comprising a heterologous regulatory region operably linked to a nucleotide sequence encoding a polypeptide, wherein said polypeptide comprises an amino acid sequence that has 98% or greater sequence identity to the amino acid sequence of SEQ ID NO: 93, and wherein a plant produced from said plant cell has an increased level of cold tolerance as compared to the corresponding level of cold tolerance of a control plant that does not comprise said nucleic acid.

2 . The plant cell of claim 1 , wherein the polypeptide comprises an amino acid sequence that has 99% or greater sequence identity to the amino acid sequence of SEQ ID NO: 93.

3 . The plant cell according to claim 1 , wherein the polypeptide comprises the amino acid sequence of SEQ ID NO: 93.

4 . A transgenic plant comprising the plant cell of claim 1 .

5 . The transgenic plant of claim 4 , wherein said polypeptide comprises an amino acid sequence that has 99% or greater sequence identity to the amino acid sequence of SEQ ID NO: 93.

6 . The transgenic plant of claim 4 , wherein said plant is a member of a species selected from the group consisting of sorghum, miscanthus, energycane, poplar, corn, soybean, canola, wheat, cotton, rice, sunflower, alfalfa, sugarbeet, and pearl millet.

7 . A seed product comprising embryonic tissue from the transgenic plant according to claim 4 , wherein the embryonic tissue comprises the exogenous nucleic acid.

8 . The transgenic plant of claim 4 , wherein said polypeptide comprises the amino acid sequence of SEQ ID NO: 93.

9 . A seed producing the transgenic plant of claim 4 .

Continuity (6)
Division 18049501 · Oct 25, 2022
Division 16829740 · Mar 25, 2020
Division 14273492 · May 8, 2014
Continuation 12863773 · Jan 21, 2009
Provisional Application 61022786 · Jan 22, 2008
Related Publication 20240318195A1 · Sep 26, 2024
References Cited (32)
US 11542521B2 · Christensen · 2023 [cited by applicant]
US 11761013B2 · Christensen et al. · 2023 [cited by applicant]
US 12024713B2 · Christensen et al. · 2024 [cited by applicant]
US 20060057724A1 · Alexandrov et al. · 2006 [cited by applicant]
US 20060150283A1 · Alexandrov · 2006 [cited by applicant]
US 20070039067A1 · Feldmann · 2007 [cited by applicant]
US 20070214517A1 · Alexandrov et al. · 2007 [cited by applicant]
US 20100192237A1 · Ren et al. · 2010 [cited by applicant]
US 20130042367A1 · Nadzan et al. · 2013 [cited by applicant]
US 20200239904A1 · Christensen et al. · 2020 [cited by applicant]
EP 1033405 · 2000 [cited by applicant]
WO 2007044988 · 2007 [cited by applicant]
Dissmeyer et al, “T-Loop Phosphorylation of Arabidopsis CDKA;1 is required for its function and can be partially substituted by an aspartate residue,”. The Plant Cell vol. 19:972-985; Mar. 2007. [cited by applicant]
Friedberg, “Automated protein function prediction- the genomic challenge,” Briefings in Bioinformatics; vol. 7. No. 3. 225-242; Jan. 2006. [cited by applicant]
Guerois et al., “Predicting changes in the stability of proteins and protein complexes: A study of more than 1000 mutations”, J. Mol. Biol.; 320; 369-287; 2002. [cited by applicant]
Kumar et al., “Predicting the effects of coding non-synonymous variants on protein function using the SIFT algorithm,” www.nature.com/natureprotocols; Nature Protocols; vol. 4 No. 8; 2009. [cited by applicant]
Ng et al., Predicting the effects of amino acid substitutions on protein function, Annual Review Genom. Hum. Genet; 7:61-80; 2006. [cited by applicant]
Reva et al., Predicting the functional impact of protein mutations: application to cancergenomics,: Nucleic Acids Research; vol. 39. No 17.; e118; Jul. 2011. [cited by applicant]
Rivera et al., Genomic evidence for two functionally distinct gene classes, Proc. Natl. Acad. Sci.; vol. 95; 6239-6244; May 1998. [cited by applicant]
Sandhya et al., “CUSP: an algorithm to distinguish structurally conserved and unconserved regions in protein domain alignments and its application in the study of large length variations,” BMC Structural Biol: 8:28; May… [cited by applicant]
Churchman, et al., SIAMESE, A plant-specific cell cycle regulator, controls endoreplication onset in [cited by applicant]
Gong, et al., “RNA helicase-like protein as an early regulator of transcription factor for plant chilling and freezing tolerance”, PNAS, vol. 99, No. 17, Aug. 20, 2002, pp. 11507-11512. [cited by applicant]
Low, et al., Conformational switch upon phosphorylation: human CDK inhibitor p191NK4d between the native and partially folded state, ACS Chemical Biology, 2008, 4(1): 53-63. [cited by applicant]
NCBI GenBank Acession AAM6430, 5, Jan. 27, 2006. [cited by applicant]
Salaita, et al., “Identification and characterization of mutants capable of seed germination at 10° C from activation-tagged lines of [cited by applicant]
Yi, et al., “The pepper transcription factor CaPF1 confers pathogen and freezing tolerance in [cited by applicant]
Churchman et al. SIAMESE, a plant-specific cell cycle regulator, controls endoreplication onset in [cited by applicant]
Peres et al. Novel plant-specific cyclin-dependent kinase inhibitors induced by biotic and abiotic stresses. Journal of Biological Chemistry. 2007. 282(35):25588-25596. [cited by applicant]
GenBank Accession No. Q9LZ60. SIAMESE-related 3. published Feb. 17, 2016. pp. -3. [cited by applicant]
GenBank Accession No. BX833341. published Feb. 6, 2004. pp 1-2. [cited by applicant]
Feldmann et al., Published Applications Database, US Publication No. 20070039067, Feb. 15, 2007, SEQ ID No. 56571. [cited by applicant]
USPTO: Non-Final Office Action regarding U.S. Appl. No. 16/829,755, dated Feb. 7, 2023. [cited by applicant]