Carbonic anhydrase-catalyzed isotope equilibrium between CO2—H2O for oxygen isotope analyses of aqueous samples
The present application relates to a method for improving analytical efficiency and cost effectiveness of oxygen isotope analysis of an aqueous sample. In particular, the present application relates to a method of determining an oxygen isotope composition of an aqueous sample by (a) equilibrating the aqueous sample with CO 2 gas wherein the aqueous sample comprises an effective amount of carbonic anhydrase (CA) enzyme; and (b) measuring the oxygen isotope composition of the CO 2 at equilibrium, wherein the oxygen isotope composition of the CO 2 corresponds to the oxygen isotope composition of the aqueous sample.
1. A method of determining an oxygen isotope composition of an aqueous sample comprising:
(a) equilibrating the aqueous sample with CO 2 gas in a vessel, wherein the aqueous sample comprises an effective amount of carbonic anhydrase (CA) enzyme and the CO 2 gas is located in a space in contact with and adjacent to the aqueous sample in the vessel; and
(b) measuring the oxygen isotope composition of the CO 2 gas at equilibrium by sampling the CO 2 gas from the space in the vessel,
wherein the oxygen isotope composition of the CO 2 gas corresponds to the oxygen isotope composition of the aqueous sample and the effective amount of the CA enzyme is an amount that catalyzes the equilibrium of CO 2 gas and H 2 O.
2. The method of claim 1 , wherein the aqueous sample is deionized water, freshwater, seawater, or brine.
3. The method of claim 2 , wherein the aqueous sample is seawater.
4. The method of claim 3 , wherein the seawater is artificial seawater or desalinized deep seawater.
5. The method of claim 2 , wherein the aqueous sample is freshwater.
6. The method of claim 5 , wherein the freshwater is iceberg water.
7. The method of claim 1 , wherein the effective amount of carbonic anhydrase (CA) enzyme is about 2 μmolal to about 10 μmolal.
8. The method of claim 1 , wherein the effective amount of carbonic anhydrase (CA) enzyme is about 4 μmolal.
9. The method of claim 1 , wherein measuring the oxygen isotope composition is performed using a mass spectrometer.
10. The method of claim 9 , wherein, the mass spectrometer is a stable isotope ratio mass spectrometer.
11. The method of claim 9 , wherein the mass spectrometer is a continuous flow isotope ratio mass spectrometer (CF-IRMS).
12. The method of claim 1 , wherein the equilibrating occurs in less than about one hour.
13. The method of claim 1 , wherein the equilibrating occurs in about 20 minutes.
14. The method of claim 1 , wherein the equilibrating is performed at ambient temperature.
15. The method of claim 14 , wherein the ambient temperature is about 25° C.
16. The method of claim 1 , wherein the oxygen isotope composition of the CO 2 gas at equilibrium of the aqueous sample comprising an effective amount of carbonic anhydrase (CA) enzyme is substantially the same as the oxygen isotope composition of the CO 2 gas at equilibrium of an aqueous sample without CA.
17. A kit for determining an oxygen isotope composition of an aqueous sample, the kit comprising carbonic anhydrase and instructions for determining the oxygen isotope composition of the aqueous sample using the method of claim 1 .
18. The method of claim 1 , wherein the effective amount of carbonic anhydrase (CA) enzyme is greater than about 2 μmolal.