Biosensor array for the detection of analytes
Embodiments of the present disclosure relate generally devices for detecting analytes in a subject. More particularly, the present disclosure provides a biosensor array for detecting analytes in a subject. Embodiments of the present disclosure include a biosensor array comprising a plurality of sensor cells for detecting an analyte in a subject. In accordance with these embodiments, the plurality of sensor cells comprises at least one electrode, at least one antibody immobilized on a surface of the at least one electrode, and a biodegradable coating in contact with the at least one antibody.
1. An insulin concentration monitoring system, the system comprising:
an implantable biosensor operably linked to an external device, and a power source;
the implantable biosensor comprises:
an implantable biosensor array comprising a plurality of sensor cells comprising at least one working electrode, a counter electrode, and a reference electrode;
one or more insulin-specific antibodies immobilized on a surface of the at least one working electrode;
each sensor cell containing a biodegradable gelatin coating of varying thicknesses in direct contact with the one or more insulin-specific antibodies; and
where the various thicknesses of biodegradable gelatin coating have different degradation rates over a period of 1 week to 6 months upon subcutaneous implantation in a subject; and
the external device comprises a microcontroller or a microcomputer-based system, capable of communicating with the implantable biosensor.
2. The system of claim 1 , wherein the insulin concentration monitoring system is configured such that electrical current is measured by the implantable biosensor; the electrical current is transmitted to the external device, the external device converts the electrical current into an impedance value; and the impedance value is used to calculate insulin concentrations using a calibration curve.
3. The system of claim 1 , wherein the power source comprises one or more batteries.
4. The system of claim 1 , wherein the external device comprises a display for displaying insulin concentrations.
5. The system of claim 1 , wherein the external device receives and records electrical current from the implantable biosensor, converts the electrical current to insulin concentrations, and displays or records the insulin concentration.
6. The system of claim 1 , wherein the implantable biosensor communicates with the external device using electromagnetic, optical, radio frequency, digital, or analog signals.
7. The system of claim 6 , wherein the communication is transmitted via a wired or wireless connection.
8. The system of claim 1 , wherein the biosensor array comprises at least 72 sensor cells.
9. The system of claim 1 , wherein the biosensor array comprises 96 sensor cells.
10. The system of claim 1 , wherein the biosensor array is about 0.1 mm-10 mm thick.
11. The system of claim 1 , wherein the biosensor array is about 0.1 mm-5 mm thick.
12. A method for monitoring insulin concentration in a subject using the system of claim 1 , the method comprising:
subcutaneously implanting the implantable biosensor in the subject;
permitting the biodegradable gelatin to degrade over the period of 1 week to 6 months exposing one or more antibody binding sites of the one or more insulin-specific antibodies; and
monitoring insulin concentrations in the subject using the external device.
13. A method for monitoring insulin concentrations in a subject, the method comprising:
providing a power source and an external device comprising a microcontroller or a microcomputer-based system, capable of communicating with an implantable biosensor;
subcutaneously implanting the implantable biosensor in the subject;
the implantable biosensor comprising:
an implantable biosensor array comprising a plurality of sensor cells comprising at least one working electrode, a counter electrode, and a reference electrode;
one or more insulin-specific antibodies immobilized on a surface of the at least one working electrode;
each sensor cell containing a biodegradable gelatin coating of varying thicknesses in direct contact with the one or more insulin-specific antibodies; and
the various thicknesses of biodegradable gelatin coating degrade at different rates over a period of 1 week to 6 months thereby exposing the insulin-specific antibodies at varying time points;
permitting the biodegradable gelatin to degrade over the period of 1 week to 6 months exposing one or more antibody binding sites of the one or more insulin-specific antibodies; and
monitoring the insulin concentrations in the subject using the external device.
14. The method of claim 13 , wherein electrical current is measured by the implantable biosensor; the electrical current is transmitted to the external device, the external device converts the electrical current into an impedance value; and the impedance value is used to calculate the insulin concentrations using a calibration curve.
15. The method of claim 13 , wherein the power source comprises one or more batteries.
16. The method of claim 13 , wherein the external device comprises a display for displaying insulin concentrations.
17. The method of claim 13 , wherein the external device receives and records electrical current from the implantable biosensor, converts the electrical current to insulin concentrations, and displays or records the insulin concentrations.
18. The method of claim 13 , wherein the implantable biosensor communicates with the external device using electromagnetic, optical, radio frequency, digital, or analog signals.
19. The method of claim 18 , wherein the communication is transmitted via a wired or wireless connection.