ECM hydrogel for treating esophageal inflammation
Methods are disclosed for inhibiting esophageal inflammation in a subject, that include administering to the esophagus of the subject with esophageal inflammation a therapeutically effective amount of an extracellular matrix (ECM) hydrogel. Methods are also disclosed for reducing esophageal stricture. Compositions are disclosed that include an esophageal extracellular matrix (ECM) hydrogel.
1. A method for inhibiting esophageal inflammation or reducing esophageal stricture in an esophagus of a subject, comprising:
administering to the esophagus of the subject a therapeutically effective amount of an extracellular matrix (ECM) digest solution comprising a hydrated, decellularized, enzymatically digested, extracellular matrix that has not been cross-linked or dialyzed, wherein the digest solution has a pH of about 7.2 to about 7.8 and forms a gel when warmed to a temperature greater than 25° C.,
thereby inhibiting esophageal inflammation or reducing esophageal stricture in the subject.
2. The method of claim 1 , wherein the ECM digest solution is terminally sterilized.
3. The method of claim 1 , wherein the ECM digest solution forms a gel when warmed to about 37° C.
4. The method of claim 1 , wherein the extracellular matrix is derived from urinary bladder, intestine, liver, esophagus, or dermis.
5. The method of claim 1 , wherein the extracellular matrix is from a monkey, horse, pig, cow, or sheep.
6. The method of claim 1 , wherein the digest solution is administered to the subject orally by swallowing or gavage.
7. The method of claim 1 , wherein the digest solution is administered to the esophagus by endoscopy or catheter.
8. The method of claim 1 , wherein the digest solution forms a gel that coats the mucosa of the esophagus upon administration to the subject at about 37° C.
9. The method of claim 1 , wherein the subject has Barret's esophagus.
10. The method of claim 1 , wherein the enzymatically digested ECM is present in the digest solution at a concentration of about 2 mg/mL to about 16 mg/mL.
11. The method of claim 1 , wherein the digest solution is administered at about 37° C.
12. The method of claim 1 , wherein the ECM digest solution is maintained below 37° C. prior to administering to the subject.
13. The method of claim 1 , wherein the ECM digest solution is maintained at or below 25° C. prior to administering to the subject.
14. The method of claim 1 , wherein the subject does not exhibit symptoms of esophageal adenocarcinoma (EAC).
15. The method of claim 1 , wherein the subject does not have, or has not previously had, gastroesophageal reflux disease.
16. The method of claim 1 , wherein the subject has dysplasia of the esophagus.
17. The method of claim 1 , further comprising administering to the subject a therapeutically effective amount of a proton pump inhibitor and/or a histamine antagonist.
18. The method of claim 1 , wherein administering the therapeutically effective amount of the ECM digest solution coats a surface of the lumen of the esophagus.
19. The method of claim 1 , further comprising:
measuring inflammation in the esophagus of the subject.