Compositions and methods for cell-like calibration particles
A method includes calibrating a cytometric device for analysis of a target cell, by inserting, into the cytometric device, a hydrogel particle. The hydrogel particle has at least one of a background fluorescent property or a spectral property that is substantially similar to the at least one of a background fluorescent property or a spectral property of the target cell. The method also includes measuring at least one property of the hydrogel particle using the cytometric device.
1. A composition, comprising:
a hydrogel particle having (1) a background fluorescent property that is substantially similar to a background fluorescent property of a target cell, and (2) a spectral property that is substantially similar to a spectral property of the target cell, the hydrogel particle including a monomer selected from hydroxyethyl methacrylate, ethyl methacrylate, 2-hydroxyethyl methacrylate (HEMA), propylene glycol methacrylate, acrylamide, N-vinylpyrrolidone (NVP), methyl methacrylate, glycidyl methacrylate, glycerol methacrylate (GMA), glycol methacrylate, ethylene glycol, fumaric acid, 2-hydroxyethyl methacrylate, hydroxyethoxyethyl methacrylate, hydroxydiethoxyethyl methacrylate, methoxyethyl methacrylate, methoxyethoxyethyl methacrylate, methoxydiethoxyethyl methacrylate, poly(ethylene glycol) methacrylate, methoxy-poly(ethylene glycol) methacrylate, methacrylic acid, sodium methacrylate, glycerol methacrylate, hydroxypropyl methacrylate, hydroxybutyl methacrylate, phenyl acrylate, phenyl methacrylate, benzyl acrylate, benzyl methacrylate, 2-phenylethyl acrylate, 2-phenylethyl methacrylate, 2-phenoxyethyl acrylate, 2-phenoxyethyl methacrylate, phenylthioethyl acrylate, phenylthioethyl methacrylate, 2,4,6-tribromophenyl acrylate, 2,4,6-tribromophenyl methacrylate, pentabromophenyl acrylate, pentabromophenyl methacrylate, pentachlorophenyl acrylate, pentachlorophenyl methacrylate, 2,3-dibromopropyl acrylate, 2,3-dibromopropyl methacrylate, 2-naphthyl acrylate, 2-naphthyl methacrylate, 4-methoxybenzyl acrylate, 4-methoxybenzyl methacrylate, 2-benzyloxyethyl acrylate, 2-benzyloxyethyl methacrylate, 4-chlorophenoxyethyl acrylate, 4-chlorophenoxyethyl methacrylate, 2-phenoxyethoxyethyl acrylate, 2-phenoxyethoxyethyl methacrylate, N-phenyl acrylamide, N-phenyl methacrylamide, N-benzyl acrylamide, N-benzyl methacrylamide, N,N-dibenzyl acrylamide, N,N-dibenzyl methacrylamide, N-diphenylmethyl acrylamide N-(4-methylphenyl)methyl acrylamide, N-1-naphthyl acrylamide, N-4-nitrophenyl acrylamide, N-(2-phenylethyl)acrylamide, N-triphenylmethyl acrylamide, N-(4-hydroxyphenyl)acrylamide, N,N-methylphenyl acrylamide, N,N-phenyl phenylethyl acrylamide, N-diphenylmethyl methacrylamide, N-(4-methyl phenyl)methyl methacrylamide, N-1-naphthyl methacrylamide, N-4-nitrophenyl methacrylamide, N-(2-phenylethyl)methacrylamide, N-triphenylmethyl methacrylamide, N-(4-hydroxyphenyl)methacrylamide, N,N-methylphenyl methacrylamide, N,N′-phenyl phenylethyl methacrylamide, N-vinyl carbazole, 4-vinylpyridine, 2-vinylpyridine, or a combination thereof.
2. The composition of claim 1 , wherein the hydrogel particle is configured to bind to an antibody that is bound to a conjugated fluorophore.
3. The composition of claim 2 , wherein the conjugated fluorophore is a fluorochrome.
4. The composition of claim 1 , wherein the hydrogel particle is a modified hydrogel particle that has been modified to bind to at least one of an intercalating nucleic acid labeling reagent or an amine-reactive labeling reagent.
5. The composition of claim 1 , wherein the hydrogel particle has a refractive index of greater than about 1.15.
6. The composition of claim 1 , wherein the hydrogel particle has a refractive index of greater than about 1.3.
7. The composition of claim 1 , wherein the hydrogel particle has a refractive index of greater than about 1.7.
8. The composition of claim 1 , wherein the hydrogel particle has a diameter of less than about 100 μm.
9. The composition of claim 1 , wherein the hydrogel particle has a diameter of less than about 10 μm.
10. The composition of claim 1 , wherein the hydrogel particle has a diameter of less than about 1 μm.
11. The composition of claim 1 , wherein the hydrogel particle contains polymer nanoparticle additives.
12. The composition of claim 1 , wherein the hydrogel particle is a chemically functionalized hydrogel particle.
13. The composition of claim 1 , wherein the hydrogel particle comprises a free amine group.
14. The composition of claim 1 , wherein the hydrogel particle comprises allylamine.
15. The composition of claim 1 , wherein the target cell is an immune cell.
16. The composition of claim 1 , wherein the hydrogel particle is a hydrogel particle that has been modified by conjugating or attaching one of a fluorophore or a fluorochrome.
17. The composition of claim 1 , wherein the hydrogel particle has been modified to match a fluorescence profile of the target cell.
18. The composition of claim 1 , wherein the at least one of the background fluorescent property or the spectral property includes one of inter-laser delay, fluorescence response, or sort timing.
19. The composition of claim 1 , wherein the target cell is a human cell.
20. A composition, comprising:
a hydrogel particle having (1) a background fluorescent property that is substantially similar to a background fluorescent property of a target cell, and (2) a spectral property that is substantially similar to a spectral property of the target cell, the hydrogel particle being a modified hydrogel particle that has been modified to bind to at least one of an intercalating nucleic acid labeling reagent or an amine-reactive labeling reagent.
21. A composition, comprising:
a hydrogel particle having (1) a background fluorescent property that is substantially similar to a background fluorescent property of a target cell, and (2) a spectral property that is substantially similar to a spectral property of the target cell, the hydrogel particle having a refractive index of greater than about 1.15.
22. A composition, comprising:
a hydrogel particle having (1) a background fluorescent property that is substantially similar to a background fluorescent property of a target cell, and (2) a spectral property that is substantially similar to a spectral property of the target cell, the hydrogel particle having a refractive index of greater than about 1.3.
23. A composition, comprising:
a hydrogel particle having (1) a background fluorescent property that is substantially similar to a background fluorescent property of a target cell, and (2) a spectral property that is substantially similar to a spectral property of the target cell, the hydrogel particle having a refractive index of greater than about 1.7.
24. A composition, comprising:
a hydrogel particle having (1) a background fluorescent property that is substantially similar to a background fluorescent property of a target cell, and (2) a spectral property that is substantially similar to a spectral property of the target cell, the hydrogel particle including a polymer nanoparticle additive.
25. A composition, comprising:
a hydrogel particle having (1) a background fluorescent property that is substantially similar to a background fluorescent property of a target cell, and (2) a spectral property that is substantially similar to a spectral property of the target cell, the hydrogel particle comprising a free amine group.
26. A composition, comprising:
a hydrogel particle having (1) a background fluorescent property that is substantially similar to a background fluorescent property of a target cell, and (2) a spectral property that is substantially similar to a spectral property of the target cell, the hydrogel particle comprising allylamine.
27. A composition, comprising:
a hydrogel particle having (1) a background fluorescent property that is substantially similar to a background fluorescent property of a target cell, and (2) a spectral property that is substantially similar to a spectral property of the target cell, the hydrogel particle being a hydrogel particle that has been modified by conjugating or attaching one of a fluorophore or a fluorochrome.
28. A composition, comprising:
a hydrogel particle having (1) a background fluorescent property that is substantially similar to a background fluorescent property of a target cell, and (2) a spectral property that is substantially similar to a spectral property of the target cell, the hydrogel particle being a hydrogel particle that has been modified to match a fluorescence profile of the target cell.