Marker, immunoassay method, immunoassay reagent, method for assaying analyte, analyte measurement kit, and lateral-flow chromatographic test strip
View Patent ↗A resin-metal composite to be used as a marker in an immunoassay, said resin-metal composite comprising a resin particle and metal particles and having the following constitution (A) or constitution (B): (A) the average particle size of the resin-metal composite exceeding 300 nm; or (B) the average particle size of the metal particles being in the range of more than 20 nm and less than 70 nm. It is preferred that a part of the metal particles are two- or three-dimensionally distributed in the surface layer part of the resin particle, a part of the three-dimensionally distributed metal particles are partly exposed to the outside of the resin particle, and a part of the remainder particles are enclosed in the resin particle.
1. A marker, comprising a resin-metal composite with a structure formed by immobilizing metal particles on a resin particle, characterized by
having constitution (A):
(A) the average particle size of the resin-metal composite being in a range of 300 nm to 1000 nm, and the average particle size of the metal particles being in a range of 1 nm to 80 nm,
wherein the metal particles comprise: metal particles having a portion embedded in the resin particle and a portion exposed from the resin particle, and metal particles adsorbed on a surface of the resin particle, wherein an amount of the metal particles adsorbed on the surface of the resin particle is 20 wt % or less relative to the total metal particles,
the metal particles are gold particles, and
the resin particle is a particle comprising a nitrogenous polymer, the nitrogenous polymer being selected from the group consisting of poly-2-vinylpyridine, poly-3-vinylpyridine, poly-4-vinylpyridine, poly-2-(diisopropylamino)ethyl methacrylate, polypyrrole, polyamide, polyamic acid, and polyimide.
2. The marker according to claim 1 , wherein the metal particles further comprise metal particles completely encased in the resin particle.
3. The marker according to claim 1 , wherein relative to the weight of the resin-metal composite, the quantity of the carried metal particles is 5 wt % to 70 wt %.
4. The marker according to claim 1 , wherein the resin-metal composite is dispersed in water.
5. The marker according to claim 1 , wherein the marker is used in adsorbing an antigen or an antibody on the surface of the resin-metal composite.
6. An immunoassay method, characterized by using the marker according to claim 1 .
7. An immunoassay reagent, comprising the marker according to claim 1 .
8. A method for measuring analyte, assaying or quantifying an analyte contained in a sample, characterized by
using a lateral-flow chromatographic test strip comprising a membrane and a determination portion formed by immobilizing capturing ligands specifically bound with the analyte on the membrane to carry out steps including step (I) to step (III) hereinafter:
step (I): step of making the analyte contained in the sample contact with a marked antibody formed by utilizing the marker according to claim 1 to mark an antibody specifically bound with the analyte;
step (II): step of making the composite containing the analyte and the marked antibody Ruined in step (I) contact with the capturing ligands in the determination portion;
step (III): step of determining colored intensity derived from the localized surface plasmon resonance of the resin-metal composite in the marker.
9. An analyte measurement kit, using a lateral-flow chromatographic test strip, used to assay or quantify an analyte contained in a sample, used to assay or quantify the analyte, and the analyte measurement kit comprising:
the lateral-flow chromatographic test strip, comprising a membrane and a determination portion formed by immobilizing capturing ligands specifically bound with the analyte on the membrane; and
an assay reagent, containing a marked antibody formed by utilizing the marker according to claim 1 to mark an antibody specifically bound with the analyte.
10. A lateral-flow chromatographic test strip, used to assay or quantify an analyte contained in a sample, comprising
a membrane;
a determination portion, formed by immobilizing capturing ligands specifically bound with the analyte on the membrane in the spreading direction of a sample; and
a reaction portion, comprising a marked antibody formed by utilizing the marker according to claim 1 to mark an antibody specifically bound with the analyte further upstream than the determination portion.