Method and carrier complexes for delivering molecules to cells
The invention relates to carrier complexes and methods for delivering molecules to cells. The carrier complexes comprises a molecule and an aromatic cationic peptide in accordance with the invention. In one embodiment, the method for delivering a molecule to a cell comprises contacting the cell with a carrier complex. In another embodiment, the method for delivering a molecule to a cell comprises contacting the cell with a molecule and an aromatic cationic peptide.
1. A carrier complex comprising a molecule conjugated to an aromatic cationic peptide, wherein the aromatic cationic peptide is selected from the group consisting of Phe-D-Arg-Phe-Lys-NH 2 (Phe 1 -DALDA), D-Arg-2′,6′-Dmt-Lys-Phe-NH 2 , and 2′,6′-Dmp-D-Arg-Phe-Lys-NH 2 (Dmp 1 -DALDA); wherein the molecule is a lipid, and where the carrier complex is formulated to deliver the lipid to a cell.
2. The carrier complex according to claim 1 , wherein the peptide is Phe-D-Arg-Phe-Lys-NH 2 (Phe 1 -DALDA).
3. The carrier complex according to claim 1 , wherein the peptide is D-Arg-2′,6′-Dmt-Lys-Phe-NH 2 .
4. The carrier complex according to claim 1 , wherein the peptide is 2′,6′-Dmp-D-Arg-Phe-Lys-NH 2 (Dmp 1 -DALDA).
5. The carrier complex according to claim 1 , wherein the cell is a bacterial cell.
6. The carrier complex according to claim 1 , wherein the cell is a plant cell.
7. The carrier complex according to claim 1 , wherein the cell is an animal cell.
8. The carrier complex according to claim 7 , wherein the animal cell is a mammalian cell.
9. The carrier complex according to claim 7 , wherein the cell is a neuronal cell.
10. The carrier complex according to claim 7 , wherein the cell is a renal epithelial cell.
11. The carrier complex according to claim 7 , wherein the cell is an intestinal epithelial cell.
12. The carrier complex according to claim 7 , wherein the cell is a vascular endothelial cell.
13. The carrier complex according to claim 12 , wherein the vascular endothelial cell is a blood-brain barrier endothelial cell.
14. The carrier complex according to claim 7 , wherein the cell is a glial cell.
15. The carrier complex according to claim 7 , wherein the cell is a hepatocyte.
16. The carrier complex according to claim 1 , wherein the aromatic-cationic peptide comprises a linker.
17. The carrier complex according to claim 1 , wherein the molecule comprises a linker.
18. The carrier complex according to claim 1 , wherein the molecule and aromatic cationic peptide are chemically bonded.