Processes for producing a fermentation product
The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and optionally a thermostable protease, pullulanase and/or glucoamylase are present and/or added during liquefaction, wherein a cellulolytic composition is present and/or added during fermentation or simultaneous saccharification and fermentation. The invention also relates to a composition suitable for use in a process of the invention.
1. A cellulolytic composition comprising:
(a) a beta-glucosidase having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 22 and having the substitutions F100D, S283G, N456E and F512Y; and
(b) a cellobiohydrolase having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 24; and
(c) an endoglucanase.
2. The cellulolytic composition of claim 1 , wherein the composition is derived from a strain of Trichoderma.
3. The cellulolytic composition of claim 1 , wherein the composition is derived from a strain of Trichoderma reesei.
4. The cellulolytic composition of claim 1 , wherein the beta-glucosidase has an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 22.
5. The cellulolytic composition of claim 1 , wherein the beta-glucosidase has an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO: 22.
6. The cellulolytic composition of claim 1 , wherein the cellobiohydrolase has an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 24.
7. The cellulolytic composition of claim 1 , wherein the cellobiohydrolase has an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO: 24.
8. A process for producing ethanol from corn, the process comprising:
(i) liquefying the corn at a temperature above the initial gelatinization temperature using a thermostable alpha-amylase and a thermostable protease;
(ii) saccharifying using a glucoamylase; and
(iii) fermenting with a yeast, wherein a cellulolytic composition is present or added during saccharifying step (ii) and/or fermenting step (iii), wherein the cellulolytic composition comprises:
(a) a beta-glucosidase having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 22 and having the substitutions F100D, S283G, N456E and F512Y; and
(b) a cellobiohydrolase having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 24; and
(c) an endoglucanase.
9. The process of claim 8 , wherein the composition is derived from a strain of Trichoderma.
10. The process of claim 8 , wherein the composition is derived from a strain of Trichoderma reesei.
11. The process of claim 8 , wherein the beta-glucosidase has an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 22.
12. The process of claim 8 , wherein the beta-glucosidase has an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO: 22.
13. The process of claim 8 , wherein the cellobiohydrolase has an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 24.
14. The process of claim 8 , wherein the cellobiohydrolase has an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO: 24.