IP Library › Granted Patent US 12,637,699
Granted Patent B2
US 12,637,699 · App. 18/637,805 · Granted May 26, 2026

Processes for producing fermentation products

Inventors: Randall Scott Deinhammer (Wake Forest, NC); Joyce Craig (Pittsboro, NC); Suzanne Clark (Youngsville, NC); John Matthews (Louisburg, NC); Anne Glud Hjulmand (Bagsvaerd, DK); Chee-Leong Soong (Raleigh, NC); Zhengfang Kang (Raleigh, NC)
Assignee: Novozymes A/S
C12P7/14C12N9/2414C12N9/2417C12N9/2457C12N9/52C12N9/58C12N9/62C12P7/06C12P7/08C12P19/14C13K1/06C12P2201/00C12P2203/00Y02E50/10
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Quick Facts
Patent No.
US 12,637,699
App. No.
18/637,805
Granted
May 26, 2026
Kind
B2
Abstract

The present invention relates to processes for producing fermentation products from starch-containing material, wherein an alpha-amylase and optionally a thermostable protease, pullulanase and/or glucoamylase are present and/or added during liquefaction, wherein a cellulolytic composition is present and/or added during fermentation or simultaneous saccharification and fermentation. The invention also relates to a composition suitable for use in a process of the invention.

Claims (37)

1 . A cellulolytic composition comprising:

(a) a beta-glucosidase having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 22 and having the substitutions F100D, S283G, N456E and F512Y; and

(b) a cellobiohydrolase I (“CBHI”) having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 24; and

(c) a cellobiohydrolase II (“CBHII”) having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 25; and

a glucoamylase.

2 . The composition of claim 1 , wherein the composition is derived from a strain of Trichoderma.

3 . The composition of claim 1 , wherein the composition is derived from a strain of Trichoderma reesei.

4 . The composition of claim 1 , wherein the beta-glucosidase has an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 22.

5 . The composition of claim 1 , wherein the beta-glucosidase has an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO: 22.

6 . The composition of claim 1 , further comprising a GH61 polypeptide having cellulolytic enhancing activity.

7 . The composition of claim 6 , wherein the GH61 polypeptide has an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 23.

8 . The composition of claim 6 , wherein the GH61 polypeptide has an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 23.

9 . The composition of claim 6 , wherein the GH61 polypeptide has an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO: 23.

10 . The composition of claim 1 , wherein the CBHI has an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 24.

11 . The composition of claim 1 , wherein the CBHI has an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO: 24.

12 . The cellulolytic composition of claim 1 , wherein the CBHII has an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 25.

13 . The cellulolytic composition of claim 1 , wherein the CBHII has an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO: 25.

14 . A process for producing ethanol from corn, the process comprising:

(i) liquefying the corn at a temperature above the initial gelatinization temperature using a thermostable alpha-amylase and a thermostable protease;

(ii) saccharifying using a glucoamylase; and

(iii) fermenting with a yeast, wherein a cellulolytic composition is present or added during saccharifying step (ii) and/or fermenting step (iii), wherein the cellulolytic composition comprises:

(a) a beta-glucosidase having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 22 and having the substitutions F100D, S283G, N456E and F512Y; and

(b) a cellobiohydrolase I (“CBHI”) having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 24; and

(c) a cellobiohydrolase II (“CBHII”) having an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 25.

15 . The process of claim 14 , wherein the composition is derived from a strain of Trichoderma.

16 . The process of claim 14 , wherein the composition is derived from a strain of Trichoderma reesei.

17 . The process of claim 14 , wherein the beta-glucosidase has an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 22.

18 . The process of claim 14 , wherein the beta-glucosidase has an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO: 22.

19 . The process of claim 14 , wherein the cellulolytic composition further comprises a GH61 polypeptide having cellulolytic enhancing activity.

20 . The process of claim 19 , wherein the GH61 polypeptide has an amino acid sequence that is at least 85% identical to the amino acid sequence of SEQ ID NO: 23.

21 . The process of claim 19 , wherein the GH61 polypeptide has an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 23.

22 . The process of claim 19 , wherein the GH61 polypeptide has an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO: 23.

23 . The process of claim 19 , wherein the CBHI has an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 24.

24 . The process of claim 19 , wherein the CBHI has an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO: 24.

25 . The process of claim 19 , wherein the CBHII has an amino acid sequence that is at least 90% identical to the amino acid sequence of SEQ ID NO: 25.

26 . The process of claim 19 , wherein the CBHII has an amino acid sequence that is at least 95% identical to the amino acid sequence of SEQ ID NO: 25.

27 . The composition of claim 1 , wherein the glucoamylase comprises a blend comprising a Talaromyces emersonii glucoamylase, a Trametes cingulate glucoamylase, and a Rhizomucor pusillus alpha-amylase with Aspergillus niger glucoamylase linker and starch binding domain (SBD).

Continuity (8)
Continuation 17229246 · Apr 13, 2021
Continuation 16702746 · Dec 4, 2019
Division 15961269 · Apr 24, 2018
Division 14648477
Continuation In Part 14388595
Provisional Application 61731806 · Nov 30, 2012
Provisional Application 61617799 · Mar 30, 2012
Related Publication 20240271167A1 · Aug 15, 2024
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