IP Library › Granted Patent US 12,188,031
Granted Patent B2
US 12,188,031 · App. 17/456,465 · Granted Jan 7, 2025

Genetic regulatory element

Inventor: Hannes Bart Claeys (Ghent, BE)
Assignee: INARI AGRICULTURE TECHNOLOGY, INC.
C12N15/8223C12N15/8281C12N15/8282C12N15/8285C12N15/8286
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Quick Facts
Patent No.
US 12,188,031
App. No.
17/456,465
Granted
Jan 7, 2025
Kind
B2
Abstract

An expression increasing element comprising a 36 nucleotide DNA sequence that can be used to increase the expression of genes, and in particular to increase the expression of endogenous plant genes, in plants is disclosed. Also disclosed are plants, plant parts, and commodity plant products comprising the expression increasing element along with related methods of using the expression increasing element and plants comprising the expression increasing element.

Claims (6)

1. A method of producing a commodity plant product, said method comprising:

(a) processing a plant or seed comprising a DNA molecule comprising the polynucleotide sequence of SEQ ID NO:3, wherein the polynucleotide sequence of SEQ ID NO:3 is located in a polynucleotide sequence comprising a promoter, wherein the polynucleotide sequence of SEQ ID NO: 3 and the promoter are operably linked to a transcription unit and increase expression of a transcript or protein encoded by the transcription unit in a plant cell relative to a control plant cell comprising the promoter in the absence of the polynucleotide sequence of SEQ ID NO: 3; and

(b) recovering the commodity plant product from the processed plant or seed.

2. The method of claim 1 , wherein the commodity plant product is seed meal, starch, syrup, silage, oil, or protein.

3. The method of claim 1 , wherein the commodity plant product is defatted corn seed meal, non-defatted corn seed meal, defatted soybean seed meal, or non-defatted soybean seed meal.

4. A method for producing nucleic acids comprising the polynucleotide sequence of SEQ ID NO:3, the method comprising isolating nucleic acids from a plant part comprising a DNA molecule comprising the polynucleotide sequence of SEQ ID NO: 3, wherein the DNA molecule in the plant part comprising the polynucleotide sequence of SEQ ID NO:3 is located in a promoter which is operably linked to a transcription unit and increase expression of a transcript or protein encoded by the transcription unit in a plant cell relative to a control plant cell comprising the promoter in the absence of the polynucleotide sequence of SEQ ID NO:3.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 6, 2022
From: CLAEYS, HANNES BART
To: INARI AGRICULTURE TECHNOLOGY, INC.
Reel/Frame 061334/0286 →
Continuity (3)
Continuation In Part 17249437 · Mar 2, 2021
Provisional Application 63084150 · Sep 28, 2020
Related Publication 20220098605A1 · Mar 31, 2022
References Cited (11)
US 11198885B1 · Claeys · 2021 [cited by applicant]
US 20150059010A1 · Cigan et al. · 2015 [cited by applicant]
US 20150082478A1 · Cigan et al. · 2015 [cited by applicant]
WO 2018140899A1 · 2018 [cited by applicant]
WO 2018183878A1 · 2018 [cited by applicant]
Davey and Wilson. “Deconstruction of complex protein signaling switches: a roadmap toward engineering higher-order gene regulators”. Nanomed Nanobiotechnol. 9: 1-15. (Year: 2017). [cited by examiner]
Bortesi, Luisa, et al., “The CRISPR/Cas9 system for plant genome editing and beyond”, Biotechnology Advances, vol. 33, pp. 41-52, 2015. [cited by applicant]
Ellis, J.G., et al., “The ocs element: a 16 base pair palindrome essential for activity of the octopine synthase enhancer”, The EMBO Journal, vol. 6, No. 11, pp. 3203-3208, 1987. [cited by applicant]
Ellis, Jeffrey G., et al., “Does the ocs-element occur as a functional component of the promoters of plant genes?”, The Plant Journal, vol. 4, No. 3, pp. 433-443, 1993. [cited by applicant]
Singh, Karambir, et al., “Saturation mutagenesis of the octopine synthase enhancer: Correlation of mutant phenotypes with binding of a nuclear protein factor”, Proc. Natl. Acad. Sci. USA, vol. 86, pp. 3733-3737, May 198… [cited by applicant]
Extended European Search Report in EP21873582.7, mailed Jul. 24, 2024, 7 pages. [cited by applicant]
Cited By (1)
US 12,747,448