Granted Patent
B2
US 12,747,448 · App. 18/166,740 · Granted Sep 29, 2026
Maize plants with improved abiotic stress tolerance
Inventors:
Richard Bailey Flavell (Malibu, CA); Yajie Niu (Lexington, MA); Michael Lee Nuccio (Salem, NH); Randall William Shultz (Acton, MA); Davide Sosso (Cambridge, IT); Maria Margarita D. Unson (Pawcatuck, CT); John Patrick Casey, Jr. (Boston, MA); Barry Andrew Martin (Newport, RI)
Assignee:
INARI AGRICULTURE TECHNOLOGY, INC.
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Abstract
The invention relates to novel maize plants, seeds and compositions, as well as improvements to maize plant breeding and methods for creating modifications in maize plant genomes.
Claims (6)
1 . A modified Zea sp. cell comprising at least one targeted modification of a first gene and at least one targeted modification of a second gene, wherein each targeted modification comprises an insertion of a transcription factor binding site, wherein the transcription factor binding site consists of SEQ ID NO: 343, wherein the targeted modifications result in increased expression of the first gene and increased expression of the second gene, and wherein the increased expression of the first and second genes are both associated with improved tolerance of abiotic stress relative to a reference cell lacking the modifications.
2 . The modified Zea sp. cell of claim 1 , wherein the first or second gene comprises a DNA sequence that is at least 95% identical to the sequence identified by SEQ ID NO:433, or encodes a protein that has an amino acid sequence that is at least 95% identical to the amino acid sequence identified by SEQ ID NO:434.
3 . The modified Zea sp. cell of claim 1 , wherein the targeted modification is of coding sequence or of non-coding sequence.
4 . The modified Zea sp. cell of claim 1 , wherein the targeted modification is determined relative to a parent Zea sp. cell, and wherein the modified Zea sp. cell is devoid of mitotically or meiotically generated genetic or epigenetic changes relative to the parent Zea sp. cell.
5 . A modified Zea sp. plant grown from the modified Zea sp. cell of claim 1 , or a progeny plant or progeny seed of the modified plant, wherein cells of the modified plant, progeny plant, or progeny seed comprise the targeted modifications.
6 . The modified Zea sp. cell of claim 1 , wherein the first or second gene comprises a DNA sequence that is at least 95% identical to the sequence identified by SEQ ID NO:435, or encodes a protein that has an amino acid sequence that is at least 95% identical to the amino acid sequence identified by SEQ ID NO:436.
Continuity (3)
Provisional Application
62566180
· Sep 29, 2017
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