IP Library › Granted Patent US 12,203,136
Granted Patent B2
US 12,203,136 · App. 18/345,629 · Granted Jan 21, 2025

Methods and systems for spatial mapping of genetic variants

Inventors: Michele A. Busby (Needham, MA); Evan Daugharthy (Cambridge, MA); Richard Terry (Carlisle, MA)
Assignee: READCOOR, LLC
C12Q1/6881C12Q1/6837C12Q1/6841C12Q2600/106C12Q2600/156C12Q2600/16
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,203,136
App. No.
18/345,629
Granted
Jan 21, 2025
Kind
B2
Abstract

The present disclosure provides methods and systems for analyzing a biological sample from a subject, comprising using probes to identify and determine the location of different genetic sequences.

Claims (23)

1. A method of analyzing a biological sample from a subject, comprising:

(a) providing a biological sample comprising multiple different populations of cells, wherein a population of cells of the multiple different populations of cells comprises a first nucleic acid sequence and a second nucleic acid sequence, wherein the first nucleic acid sequence corresponds to a major histocompatibility complex (MHC) variant sequence;

(b) coupling a first probe to the first nucleic acid sequence and coupling a second probe to the second nucleic acid sequence; and

(c) detecting the first probe and the second probe, thereby determining that the first nucleic acid sequence and the second nucleic acid sequence are co-localized within the population of cells in the biological sample.

2. The method of claim 1 , wherein the second nucleic acid sequence comprises a mutation.

3. The method of claim 1 , wherein the second nucleic acid sequence corresponds to a marker of inflammation.

4. The method of claim 1 , wherein the second nucleic acid sequence corresponds to a marker for cell typing.

5. The method of claim 1 , wherein the second nucleic acid sequence corresponds to a marker for an immune cell.

6. The method of claim 1 , wherein the second nucleic acid sequence corresponds to an additional MHC variant sequence.

7. The method of claim 6 , further comprising identifying a clone within the biological sample based on a first location of the first nucleic acid sequence and a second location of the second nucleic acid sequence.

8. The method of claim 7 , further comprising generating a visual representation of a third location of the clone within the biological sample for display on a graphical user interface (GUI).

9. The method of claim 6 , wherein the second nucleic acid sequence comprises an MHC allele.

10. The method of claim 1 , further comprising generating a visual representation of a first location of the first nucleic acid sequence and a second location of the second nucleic acid sequence for display on a graphical user interface (GUI).

11. The method of claim 1 , further comprising identifying a cell or derivative thereof within the biological sample, wherein the cell or derivative thereof comprises the first nucleic acid sequence and the second nucleic acid sequence.

12. The method of claim 1 , further comprising predicting a presentation of a peptide on an MHC protein expressed in the biological sample, wherein the peptide is at least partially encoded by the second nucleic acid sequence and the MHC protein is at least partially encoded by the first nucleic acid sequence.

13. The method of claim 12 , wherein the peptide is a mutant peptide.

14. The method of claim 1 , wherein the determining that the first nucleic acid sequence and the second nucleic acid sequence are co-localized within the population of cells in the biological sample further comprises identifying the first nucleic acid sequence.

15. The method of claim 14 , wherein the first nucleic acid sequence comprises an MHC allele.

16. The method of claim 1 , wherein (a) further comprises providing the biological sample within a three-dimensional (3D) matrix that preserves spatial information of the first nucleic acid sequence prior to (b).

17. The method of claim 16 , wherein the biological sample is provided within the 3D matrix by directing a precursor of the 3D matrix through the biological sample and subjecting the precursor of the 3D matrix to a reaction to generate cross-links and form the 3D matrix.

18. The method of claim 1 , further comprising, subjecting the first probe to an amplification reaction to generate an amplified nucleic acid molecule that corresponds to the MHC variant sequence.

19. The method of claim 1 , wherein the first probe is a circularizable probe.

20. The method of claim 1 , further comprising contacting the biological sample with a plurality of fluorescently labeled oligonucleotides to identify at least a portion of the first probe.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 9, 2024
From: BUSBY, MICHELE A.; DAUGHARTHY, EVAN; TERRY, RICHARD
To: READCOOR LLC
Reel/Frame 066429/0595 →
Continuity (3)
Continuation 17403405 · Aug 16, 2021
Provisional Application 63066604 · Aug 17, 2020
Related Publication 20240150835A1 · May 9, 2024
References Cited (400)
US 3501459A · Schindler et al. · 1970 [cited by applicant]
US 3551710A · Gourdine · 1970 [cited by applicant]
US 3871445A · Wanka et al. · 1975 [cited by applicant]
US 3993233A · Bartell · 1976 [cited by applicant]
US 4123610A · Summerton et al. · 1978 [cited by applicant]
US 4844617A · Kelderman et al. · 1989 [cited by applicant]
US 4886741A · Schwartz · 1989 [cited by applicant]
US 4981985A · Kaplan et al. · 1991 [cited by applicant]
US 5116742A · Cech et al. · 1992 [cited by applicant]
US 5151189A · Hu et al. · 1992 [cited by applicant]
US 5244797A · Kotewicz et al. · 1993 [cited by applicant]
US 5424413A · Hogan et al. · 1995 [cited by applicant]
US 5563056A · Swan et al. · 1996 [cited by applicant]
US 5594235A · Lee · 1997 [cited by applicant]
US 5635352A · Urdea et al. · 1997 [cited by applicant]
US 5684149A · Morrow · 1997 [cited by applicant]
US 5695940A · Drmanac et al. · 1997 [cited by applicant]
US 5795236A · Alberts et al. · 1998 [cited by applicant]
US 5830708A · Naughton · 1998 [cited by applicant]
US 5834758A · Trulson et al. · 1998 [cited by applicant]
US 5871921A · Landegren et al. · 1999 [cited by applicant]
US 6063612A · Jayasena et al. · 2000 [cited by applicant]
US 6068979A · Akhavan-Tafti · 2000 [cited by applicant]
US 6083726A · Mills, Jr. et al. · 2000 [cited by applicant]
US 6194148B1 · Hori et al. · 2001 [cited by applicant]
US 6221603B1 · Mahtani · 2001 [cited by applicant]
US 6232067B1 · Hunkapiller et al. · 2001 [cited by applicant]
US 6306597B1 · Macevicz · 2001 [cited by applicant]
US 6534266B1 · Singer · 2003 [cited by applicant]
US 6586176B1 · Trnovsky et al. · 2003 [cited by applicant]
US 6632655B1 · Mehta et al. · 2003 [cited by applicant]
US 7255994B2 · Lao · 2007 [cited by applicant]
US 7323305B2 · Leamon et al. · 2008 [cited by applicant]
US 7427479B2 · Karger et al. · 2008 [cited by applicant]
US 7473767B2 · Dimitrov · 2009 [cited by applicant]
US 7534991B2 · Miller et al. · 2009 [cited by applicant]
US 7555155B2 · Levenson et al. · 2009 [cited by applicant]
US 7655898B2 · Miller · 2010 [cited by applicant]
US 7745129B1 · Schatz · 2010 [cited by applicant]
US 7771949B2 · Kramer · 2010 [cited by applicant]
US 7906285B2 · Drmanac · 2011 [cited by applicant]
US 7910304B2 · Drmanac · 2011 [cited by applicant]
US 7941279B2 · Hwang et al. · 2011 [cited by applicant]
US 7989166B2 · Koch et al. · 2011 [cited by applicant]
US 8013134B2 · Fredriksson · 2011 [cited by applicant]
US 8124751B2 · Pierce et al. · 2012 [cited by applicant]
US 8199999B2 · Hoyt et al. · 2012 [cited by applicant]
US 8268554B2 · Schallmeiner · 2012 [cited by applicant]
US 8329404B2 · McKernan et al. · 2012 [cited by applicant]
US 8330087B2 · Domenicali · 2012 [cited by applicant]
US 8415102B2 · Geiss et al. · 2013 [cited by applicant]
US 8431691B2 · McKernan et al. · 2013 [cited by applicant]
US 8460865B2 · Chee et al. · 2013 [cited by applicant]
US 8462981B2 · Determan et al. · 2013 [cited by applicant]
US 8501459B2 · Chen et al. · 2013 [cited by applicant]
US 8519115B2 · Webster et al. · 2013 [cited by applicant]
US 8551710B2 · Bernitz et al. · 2013 [cited by applicant]
US 8658361B2 · Wu et al. · 2014 [cited by applicant]
US 8697359B1 · Zhang · 2014 [cited by applicant]
US 8871445B2 · Cong et al. · 2014 [cited by applicant]
US 8906616B2 · Zhang et al. · 2014 [cited by applicant]
US 8932814B2 · Cong et al. · 2015 [cited by applicant]
US 8946389B2 · Gao et al. · 2015 [cited by applicant]
US 8986926B2 · Ferree et al. · 2015 [cited by applicant]
US 8993233B2 · Zhang et al. · 2015 [cited by applicant]
US 8999641B2 · Zhang et al. · 2015 [cited by applicant]
US 9017992B2 · Winther et al. · 2015 [cited by applicant]
US 9201063B2 · Sood et al. · 2015 [cited by applicant]
US 9217151B2 · Yin et al. · 2015 [cited by applicant]
US 9232067B2 · Leigh et al. · 2016 [cited by applicant]
US 9257135B2 · Ong et al. · 2016 [cited by applicant]
US 9267135B2 · Church et al. · 2016 [cited by applicant]
US 9273349B2 · Nguyen et al. · 2016 [cited by applicant]
US 9371563B2 · Geiss et al. · 2016 [cited by applicant]
US 9371598B2 · Chee · 2016 [cited by applicant]
US 9376717B2 · Gao et al. · 2016 [cited by applicant]
US 9541504B2 · Hoyt · 2017 [cited by applicant]
US 9551032B2 · Landegren et al. · 2017 [cited by applicant]
US 9714446B2 · Webster et al. · 2017 [cited by applicant]
US 9714937B2 · Dunaway · 2017 [cited by applicant]
US 9727810B2 · Fodor et al. · 2017 [cited by applicant]
US 9778155B2 · Gradinaru et al. · 2017 [cited by applicant]
US 9783841B2 · Nolan et al. · 2017 [cited by applicant]
US 9896720B2 · Raj et al. · 2018 [cited by applicant]
US 9909167B2 · Samusik et al. · 2018 [cited by applicant]
US 9914967B2 · Church et al. · 2018 [cited by applicant]
US 10030261B2 · Frisen et al. · 2018 [cited by applicant]
US 10032064B2 · Hoyt · 2018 [cited by applicant]
US 10036055B2 · Church et al. · 2018 [cited by applicant]
US 10126242B2 · Miller et al. · 2018 [cited by applicant]
US 10138509B2 · Church et al. · 2018 [cited by applicant]
US 10179932B2 · Church et al. · 2019 [cited by applicant]
US 10227639B2 · Levner et al. · 2019 [cited by applicant]
US 10246700B2 · Dunaway et al. · 2019 [cited by applicant]
US 10266888B2 · Daugharthy et al. · 2019 [cited by applicant]
US 10267808B2 · Cai · 2019 [cited by applicant]
US 10309879B2 · Chen et al. · 2019 [cited by applicant]
US 10317321B2 · Tillberg et al. · 2019 [cited by applicant]
US 10364457B2 · Wassie et al. · 2019 [cited by applicant]
US 10370698B2 · Nolan et al. · 2019 [cited by applicant]
US 10415080B2 · Dunaway et al. · 2019 [cited by applicant]
US 10457980B2 · Cai et al. · 2019 [cited by applicant]
US 10465235B2 · Gullberg et al. · 2019 [cited by applicant]
US 10494662B2 · Church et al. · 2019 [cited by applicant]
US 10494664B2 · Doudna et al. · 2019 [cited by applicant]
US 10494667B2 · Chee · 2019 [cited by applicant]
US 10495554B2 · Deisseroth et al. · 2019 [cited by applicant]
US 10501777B2 · Beechem et al. · 2019 [cited by applicant]
US 10501791B2 · Church et al. · 2019 [cited by applicant]
US 10510435B2 · Cai et al. · 2019 [cited by applicant]
US 10526649B2 · Chen et al. · 2020 [cited by applicant]
US 10538795B2 · Seitz et al. · 2020 [cited by applicant]
US 10545075B2 · Deisseroth et al. · 2020 [cited by applicant]
US 10580128B2 · Miller · 2020 [cited by applicant]
US 10640816B2 · Beechem et al. · 2020 [cited by applicant]
US 10640826B2 · Church et al. · 2020 [cited by applicant]
US 10669569B2 · Gullberg et al. · 2020 [cited by applicant]
US 10746981B2 · Tomer et al. · 2020 [cited by applicant]
US 10774372B2 · Chee et al. · 2020 [cited by applicant]
US 10774374B2 · Frisen et al. · 2020 [cited by applicant]
US 10794802B2 · Gradinaru et al. · 2020 [cited by applicant]
US 10802262B2 · Tomer et al. · 2020 [cited by applicant]
US 10815519B2 · Husain et al. · 2020 [cited by applicant]
US 10844426B2 · Daugharthy et al. · 2020 [cited by applicant]
US 10858692B2 · Church et al. · 2020 [cited by applicant]
US 10872679B2 · Cai et al. · 2020 [cited by applicant]
US 10964001B2 · Miller · 2021 [cited by applicant]
US 11021737B2 · Church et al. · 2021 [cited by applicant]
US 11021741B2 · Wu et al. · 2021 [cited by applicant]
US 11078520B2 · Church et al. · 2021 [cited by applicant]
US 11085072B2 · Church et al. · 2021 [cited by applicant]
US 11111521B2 · Church et al. · 2021 [cited by applicant]
US 11118220B2 · Daugharthy et al. · 2021 [cited by applicant]
US 11193163B2 · Daugharthy et al. · 2021 [cited by applicant]
US 11293051B2 · Church et al. · 2022 [cited by applicant]
US 11293052B2 · Church et al. · 2022 [cited by applicant]
US 11293054B2 · Levner et al. · 2022 [cited by applicant]
US 11299767B2 · Church et al. · 2022 [cited by applicant]
US 11312992B2 · Church et al. · 2022 [cited by applicant]
US 11447807B2 · Church et al. · 2022 [cited by applicant]
US 11466310B2 · Daniel · 2022 [cited by applicant]
US 11473139B2 · Church et al. · 2022 [cited by applicant]
US 11542554B2 · Daugharthy et al. · 2023 [cited by applicant]
US 11549136B2 · Church et al. · 2023 [cited by applicant]
US 11566276B2 · Church et al. · 2023 [cited by applicant]
US 11566277B2 · Church et al. · 2023 [cited by applicant]
US 11639518B2 · Church et al. · 2023 [cited by applicant]
US 11713485B2 · Daugharthy et al. · 2023 [cited by applicant]
US 11718874B2 · Daugharthy et al. · 2023 [cited by applicant]
US 20010039018A1 · Matson et al. · 2001 [cited by applicant]
US 20020015952A1 · Anderson et al. · 2002 [cited by applicant]
US 20020029979A1 · Freund et al. · 2002 [cited by applicant]
US 20020049176A1 · Anderson et al. · 2002 [cited by applicant]
US 20020155989A1 · Efimov et al. · 2002 [cited by applicant]
US 20020168645A1 · Taylor · 2002 [cited by applicant]
US 20020172950A1 · Kenny et al. · 2002 [cited by applicant]
US 20030018984A1 · Coleman et al. · 2003 [cited by applicant]
US 20030148335A1 · Shen et al. · 2003 [cited by applicant]
US 20030165852A1 · Schueler et al. · 2003 [cited by applicant]
US 20040006035A1 · Macejak et al. · 2004 [cited by applicant]
US 20040077014A1 · Becker · 2004 [cited by applicant]
US 20040081962A1 · Chen et al. · 2004 [cited by applicant]
US 20040081979A1 · Knezevic et al. · 2004 [cited by applicant]
US 20040126770A1 · Kumar et al. · 2004 [cited by applicant]
US 20040152072A1 · Gerard · 2004 [cited by applicant]
US 20040191794A1 · Weindel · 2004 [cited by examiner]
US 20040248144A1 · Mir · 2004 [cited by applicant]
US 20040259190A1 · Naughton · 2004 [cited by applicant]
US 20040259226A1 · Robey et al. · 2004 [cited by applicant]
US 20050032694A1 · Sonderegger · 2005 [cited by applicant]
US 20050064435A1 · Su et al. · 2005 [cited by applicant]
US 20050106629A1 · McGrath et al. · 2005 [cited by applicant]
US 20050147981A1 · Yamakawa et al. · 2005 [cited by applicant]
US 20050191687A1 · Wang et al. · 2005 [cited by applicant]
US 20050221304A1 · Xiang et al. · 2005 [cited by applicant]
US 20050233318A1 · Chee et al. · 2005 [cited by applicant]
US 20060024711A1 · Lapidus et al. · 2006 [cited by applicant]
US 20060046311A1 · Sun et al. · 2006 [cited by applicant]
US 20060077536A1 · Bromage et al. · 2006 [cited by applicant]
US 20060127916A1 · Seul et al. · 2006 [cited by applicant]
US 20060177833A1 · Brenner · 2006 [cited by applicant]
US 20060183107A1 · Melkonyan · 2006 [cited by applicant]
US 20060216339A1 · Ambron et al. · 2006 [cited by applicant]
US 20060228733A1 · Pierce et al. · 2006 [cited by applicant]
US 20060234261A1 · Pierce et al. · 2006 [cited by applicant]
US 20060248349A1 · Rathjen et al. · 2006 [cited by applicant]
US 20060292611A1 · Berka et al. · 2006 [cited by applicant]
US 20070003949A1 · Rava · 2007 [cited by applicant]
US 20070020650A1 · Kahvejian · 2007 [cited by applicant]
US 20070087362A1 · Church et al. · 2007 [cited by applicant]
US 20070117109A1 · Rothemund · 2007 [cited by applicant]
US 20070117177A1 · Luo et al. · 2007 [cited by applicant]
US 20070172873A1 · Brenner et al. · 2007 [cited by applicant]
US 20070190543A1 · Livak · 2007 [cited by applicant]
US 20070206275A1 · Hemmer et al. · 2007 [cited by applicant]
US 20070212700A1 · Ranganathan et al. · 2007 [cited by applicant]
US 20070231823A1 · McKernan et al. · 2007 [cited by applicant]
US 20070292877A1 · Dimitrov · 2007 [cited by applicant]
US 20080003586A1 · Hyde et al. · 2008 [cited by applicant]
US 20080003599A1 · Dary et al. · 2008 [cited by applicant]
US 20080050718A1 · Gesteland et al. · 2008 [cited by applicant]
US 20080176769A1 · Rank et al. · 2008 [cited by applicant]
US 20080180790A1 · Tafas et al. · 2008 [cited by applicant]
US 20080269068A1 · Church et al. · 2008 [cited by applicant]
US 20090088327A1 · Rigatti et al. · 2009 [cited by applicant]
US 20090105082A1 · Chetverin et al. · 2009 [cited by applicant]
US 20090208965A1 · Tafas et al. · 2009 [cited by applicant]
US 20090220968A1 · Issadore et al. · 2009 [cited by applicant]
US 20090246879A1 · Drmanac et al. · 2009 [cited by applicant]
US 20090269739A1 · Cech et al. · 2009 [cited by applicant]
US 20090280559A1 · McCarthy · 2009 [cited by applicant]
US 20090307179A1 · Colby · 2009 [cited by examiner]
US 20090325172A1 · Milton et al. · 2009 [cited by applicant]
US 20100009868A1 · Yan et al. · 2010 [cited by applicant]
US 20100015607A1 · Geiss et al. · 2010 [cited by applicant]
US 20100028885A1 · Balasubramanian et al. · 2010 [cited by applicant]
US 20100049448A1 · Doyle et al. · 2010 [cited by applicant]
US 20100076057A1 · Sontheimer et al. · 2010 [cited by applicant]
US 20100087325A1 · Buermann · 2010 [cited by applicant]
US 20100151472A1 · Nolan et al. · 2010 [cited by applicant]
US 20100223276A1 · Al-Shameri et al. · 2010 [cited by applicant]
US 20100268478A1 · Andregg et al. · 2010 [cited by applicant]
US 20110020291A1 · Banerjee et al. · 2011 [cited by applicant]
US 20110033520A1 · Mather et al. · 2011 [cited by applicant]
US 20110090562A1 · Brooker · 2011 [cited by applicant]
US 20110092376A1 · Colston, Jr. et al. · 2011 [cited by applicant]
US 20110104693A1 · Seligmann · 2011 [cited by applicant]
US 20110189776A1 · Terns et al. · 2011 [cited by applicant]
US 20110208040A1 · Carmi et al. · 2011 [cited by applicant]
US 20110216953A1 · Callahan et al. · 2011 [cited by applicant]
US 20110223585A1 · Gullberg et al. · 2011 [cited by applicant]
US 20110223638A1 · Wiedenheft et al. · 2011 [cited by applicant]
US 20110257031A1 · Bodeau et al. · 2011 [cited by applicant]
US 20110294135A1 · Carlson · 2011 [cited by applicant]
US 20120040397A1 · Luo et al. · 2012 [cited by applicant]
US 20120122712A1 · Goldstein · 2012 [cited by applicant]
US 20120126142A1 · Matsui et al. · 2012 [cited by applicant]
US 20120252686A1 · Umbarger et al. · 2012 [cited by applicant]
US 20120270214A1 · Bernitz et al. · 2012 [cited by applicant]
US 20120330636A1 · Albou · 2012 [cited by applicant]
US 20130017229A1 · Mooney et al. · 2013 [cited by applicant]
US 20130130248A1 · Haurwitz et al. · 2013 [cited by applicant]
US 20130245096A1 · Abitbol · 2013 [cited by applicant]
US 20130253040A1 · Miller et al. · 2013 [cited by applicant]
US 20130323729A1 · Landegren et al. · 2013 [cited by applicant]
US 20140049632A1 · Hemmer · 2014 [cited by applicant]
US 20140068797A1 · Doudna et al. · 2014 [cited by applicant]
US 20140087378A1 · Chatre et al. · 2014 [cited by applicant]
US 20140087427A1 · Bujnicki et al. · 2014 [cited by applicant]
US 20140179006A1 · Zhang · 2014 [cited by applicant]
US 20140179770A1 · Zhang et al. · 2014 [cited by applicant]
US 20140200146A1 · Xie et al. · 2014 [cited by applicant]
US 20140220578A1 · Bohannon et al. · 2014 [cited by applicant]
US 20140220587A1 · Green, Jr. et al. · 2014 [cited by applicant]
US 20140270435A1 · Dunn · 2014 [cited by applicant]
US 20140273226A1 · Wu · 2014 [cited by applicant]
US 20140273233A1 · Chen et al. · 2014 [cited by applicant]
US 20140308695A1 · Bruce et al. · 2014 [cited by applicant]
US 20140310830A1 · Zhang et al. · 2014 [cited by applicant]
US 20140315985A1 · May et al. · 2014 [cited by applicant]
US 20140342457A1 · Mali et al. · 2014 [cited by applicant]
US 20150098126A1 · Keller et al. · 2015 [cited by applicant]
US 20150133319A1 · Fu et al. · 2015 [cited by applicant]
US 20150247150A1 · Zhang et al. · 2015 [cited by applicant]
US 20160002704A1 · Diehl et al. · 2016 [cited by applicant]
US 20160024524A1 · Joung et al. · 2016 [cited by applicant]
US 20160024555A1 · Church · 2016 [cited by examiner]
US 20160106439A1 · Menashe · 2016 [cited by applicant]
US 20160108458A1 · Frei et al. · 2016 [cited by applicant]
US 20160153006A1 · Zhang et al. · 2016 [cited by applicant]
US 20160160210A1 · Mali et al. · 2016 [cited by applicant]
US 20160265046A1 · Zhang et al. · 2016 [cited by applicant]
US 20160289740A1 · Fu et al. · 2016 [cited by applicant]
US 20160305856A1 · Boyden et al. · 2016 [cited by applicant]
US 20160340662A1 · Zhang et al. · 2016 [cited by applicant]
US 20160355795A1 · Ran et al. · 2016 [cited by applicant]
US 20160358326A1 · Sarachan et al. · 2016 [cited by applicant]
US 20160369321A1 · Landegren et al. · 2016 [cited by applicant]
US 20160376642A1 · Landegren et al. · 2016 [cited by applicant]
US 20170009278A1 · Söderberg et al. · 2017 [cited by applicant]
US 20170010672A1 · Tanaka et al. · 2017 [cited by applicant]
US 20170081489A1 · Rodriques et al. · 2017 [cited by applicant]
US 20170176338A1 · Wu et al. · 2017 [cited by applicant]
US 20170191078A1 · Zhang et al. · 2017 [cited by applicant]
US 20170220733A1 · Zhuang et al. · 2017 [cited by applicant]
US 20170253918A1 · Kohman · 2017 [cited by applicant]
US 20170262984A1 · Barnes et al. · 2017 [cited by applicant]
US 20180010166A1 · Pierce et al. · 2018 [cited by applicant]
US 20180052081A1 · Kohman · 2018 [cited by applicant]
US 20180080876A1 · Rockel et al. · 2018 [cited by applicant]
US 20180208967A1 · Larman et al. · 2018 [cited by applicant]
US 20180237864A1 · Imler et al. · 2018 [cited by applicant]
US 20180282787A1 · Walter et al. · 2018 [cited by applicant]
US 20190017106A1 · Frisen et al. · 2019 [cited by applicant]
US 20190032128A1 · Chen et al. · 2019 [cited by applicant]
US 20190032151A1 · Wallin · 2019 [cited by examiner]
US 20190085383A1 · Church et al. · 2019 [cited by applicant]
US 20190119735A1 · Deisseroth et al. · 2019 [cited by applicant]
US 20190130607A1 · Atchison · 2019 [cited by examiner]
US 20190155835A1 · Daugharthy et al. · 2019 [cited by applicant]
US 20190194709A1 · Church et al. · 2019 [cited by applicant]
US 20190218608A1 · Daugharthy et al. · 2019 [cited by applicant]
US 20190241938A1 · Levner et al. · 2019 [cited by applicant]
US 20190264270A1 · Zhuang et al. · 2019 [cited by applicant]
US 20190271028A1 · Khafizov et al. · 2019 [cited by applicant]
US 20190276881A1 · Zhuang et al. · 2019 [cited by applicant]
US 20190330617A1 · Church et al. · 2019 [cited by applicant]
US 20200009786A1 · Hikmet et al. · 2020 [cited by applicant]
US 20200010891A1 · Beechem et al. · 2020 [cited by applicant]
US 20200034347A1 · Selly · 2020 [cited by applicant]
US 20200090786A1 · Quiroz Zarate et al. · 2020 [cited by applicant]
US 20200140920A1 · Pierce et al. · 2020 [cited by applicant]
US 20200239946A1 · Dewal · 2020 [cited by applicant]
US 20200354782A1 · Dewal · 2020 [cited by applicant]
US 20210017587A1 · Cai et al. · 2021 [cited by applicant]
US 20210087611A1 · Church et al. · 2021 [cited by applicant]
US 20210102244A1 · Church et al. · 2021 [cited by applicant]
US 20210115504A1 · Cai et al. · 2021 [cited by applicant]
US 20210292834A1 · Daugharthy et al. · 2021 [cited by applicant]
US 20210310052A1 · Daugharthy et al. · 2021 [cited by applicant]
US 20210324450A1 · Church et al. · 2021 [cited by applicant]
US 20210332414A1 · Church · 2021 [cited by applicant]
US 20210332415A1 · Church et al. · 2021 [cited by applicant]
US 20210340621A1 · Daugharthy et al. · 2021 [cited by applicant]
US 20210363579A1 · Daugharthy et al. · 2021 [cited by applicant]
US 20210381049A1 · Daugharthy et al. · 2021 [cited by applicant]
US 20220016624A1 · Daugharthy et al. · 2022 [cited by applicant]
US 20220025448A1 · Levner et al. · 2022 [cited by applicant]
US 20220049303A1 · Busby et al. · 2022 [cited by applicant]
US 20220145365A1 · Church et al. · 2022 [cited by applicant]
US 20220228196A1 · Church et al. · 2022 [cited by applicant]
US 20220251642A1 · Church et al. · 2022 [cited by applicant]
US 20220282301A1 · Levner et al. · 2022 [cited by applicant]
US 20220298559A1 · Daugharthy et al. · 2022 [cited by applicant]
US 20230212649A1 · Church et al. · 2023 [cited by applicant]
US 20240018569A1 · Levner et al. · 2024 [cited by applicant]
US 20240124932A1 · Daugharthy et al. · 2024 [cited by applicant]
BR 112015013784A2 · 2017 [cited by applicant]
BR 112015013785A2 · 2017 [cited by applicant]
BR 112015008708A2 · 2017 [cited by applicant]
BR 112015012375A2 · 2017 [cited by applicant]
BR 112015014425A2 · 2017 [cited by applicant]
BR 112015022061A2 · 2017 [cited by applicant]
CA 2116214A1 · 1993 [cited by applicant]
CA 2891347A1 · 2014 [cited by applicant]
CN 1432069A · 2003 [cited by applicant]
CN 1580283A · 2005 [cited by applicant]
CN 1959384A · 2007 [cited by applicant]
CN 101285095A · 2008 [cited by applicant]
CN 101400803A · 2009 [cited by applicant]
CN 101553306A · 2009 [cited by applicant]
CN 101608232A · 2009 [cited by applicant]
CN 103364380A · 2013 [cited by applicant]
CN 105264073A · 2016 [cited by applicant]
CN 105392898A · 2016 [cited by applicant]
EP 2288726A2 · 2011 [cited by applicant]
EP 2465943A2 · 2012 [cited by applicant]
EP 2798092A2 · 2014 [cited by applicant]
EP 2878671A1 · 2015 [cited by applicant]
EP 3425063A1 · 2019 [cited by applicant]
EP 2794928B1 · 2019 [cited by applicant]
EP 2971184B1 · 2019 [cited by applicant]
EP 2766498B1 · 2019 [cited by applicant]
EP 3578666A1 · 2019 [cited by applicant]
EP 3847274A1 · 2021 [cited by applicant]
EP 4108782B1 · 2023 [cited by applicant]
JP H04268359A · 1992 [cited by applicant]
JP 2007526772A · 2007 [cited by applicant]
JP 2009538123A · 2009 [cited by applicant]
JP 2012170337A · 2012 [cited by applicant]
JP 2014513523A · 2014 [cited by applicant]
JP 2015090458A · 2015 [cited by applicant]
KR 20080003402A · 2008 [cited by applicant]
WO WO9746704A1 · 1997 [cited by applicant]
WO WO9856955A1 · 1998 [cited by applicant]
WO WO9961665A2 · 1999 [cited by applicant]
WO WO0126708A1 · 2001 [cited by applicant]
WO WO0137266A1 · 2001 [cited by applicant]
WO WO03003810A2 · 2003 [cited by applicant]
WO WO03044229A1 · 2003 [cited by applicant]
WO WO03102233A1 · 2003 [cited by applicant]
WO WO2004104645A2 · 2004 [cited by applicant]
WO WO2006138257A2 · 2006 [cited by applicant]
WO WO2007001986A2 · 2007 [cited by applicant]
WO WO2007076128A2 · 2007 [cited by applicant]
WO WO2007086900A2 · 2007 [cited by applicant]
WO WO2007121489A2 · 2007 [cited by applicant]
WO WO2007123744A2 · 2007 [cited by applicant]
WO WO2007149696A1 · 2007 [cited by applicant]
WO WO2008041002A2 · 2008 [cited by applicant]
WO WO2008069973A2 · 2008 [cited by applicant]
WO WO2008108989A2 · 2008 [cited by applicant]
WO WO2008157696A2 · 2008 [cited by applicant]
WO WO2009046149A1 · 2009 [cited by applicant]
WO WO2009046348A1 · 2009 [cited by applicant]
WO WO2010054108A2 · 2010 [cited by applicant]
WO WO2010080134A1 · 2010 [cited by applicant]
WO WO2010087325A1 · 2010 [cited by applicant]
WO WO2010104533A2 · 2010 [cited by applicant]
WO WO2011092596A2 · 2011 [cited by applicant]