IP Library › Granted Patent US 12,203,937
Granted Patent B2
US 12,203,937 · App. 17/264,489 · Granted Jan 21, 2025

Compositions and methods for carrying out assay measurements

Inventors: Alexander K. Tucker-Schwartz (Bethesda, MD); George Sigal (Rockville, MD)
Assignee: MESO SCALE TECHNOLOGIES, LLC.
G01N33/58G01N21/66G01N2458/30
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,203,937
App. No.
17/264,489
Granted
Jan 21, 2025
Kind
B2
Abstract

The present disclosure provides compositions, reagents, kits, systems, system components, and methods for performing assays. More particularly, the disclosure relates to an assay composition for detecting luminescence, which comprises an alkyl diethanolamine, for example, N-butyldiethanolamine (BDEA). In embodiments, the assay composition comprises 2-dibutylaminoethanol (BDAE).

Claims (31)

1. A composition comprising:

(i) about 125 mM to about 175 mM of N-butyldiethanolamine (BDEA),

(ii) a pH buffering component, and

(iii) a salt.

2. The composition of claim 1 , wherein the pH buffering component comprises tris(hydroxymethyl)aminomethane (Tris), phosphate, HEPES, glycylglycine, borate, acetate, citrate, or a combination thereof.

3. The composition of claim 1 , wherein the composition comprises a surfactant.

4. The composition of claim 3 , wherein the surfactant is about 1.0 mM to about 5.0 mM TRITON X-100.

5. The composition of claim 3 , wherein the surfactant is KOLLIPHOR P-407, PLURONIC P-123, PLURONIC L-121, PLURONIC 31R1, TETRONIC 701, 2,4,7,9-tetramethyl-d-decyne-4,7-diol ethoxylate, PEG (18) tridecyl ether, BRIJ L4, BRIJ 58, TWEEN 20, or a combination thereof.

6. The composition of claim 1 , wherein the pH of the composition is about 6 to about 9.

7. The composition of claim 6 , wherein the pH is about 7 to about 8.

8. The composition of claim 1 , wherein the salt comprises chloride ion.

9. The composition of claim 8 , wherein the salt comprises NaCl, KCl, LiCl, or a combination thereof.

10. The composition of claim 1 , wherein the composition comprises a liquid diluent, and wherein the liquid diluent comprises water.

11. The composition of claim 1 , wherein the concentration of the BDEA or DBAE is about 150 mM to about 175 mM.

12. The composition of claim 1 , wherein the concentration of the pH buffering component is about 100 mM to about 300 mM.

13. The composition of claim 1 , wherein the concentration of the pH buffering component is about 150 mM to about 250 mM.

14. The composition of claim 1 , wherein the composition has an ionic strength of greater than about 0.5 M.

15. The composition of claim 1 , wherein the composition has an ionic strength of greater than about 0.8 M.

16. The composition of claim 8 , wherein the concentration of the chloride ion is greater than about 0.5 M.

17. The composition of claim 8 , wherein the concentration of the chloride ion is greater than about 0.75 M.

18. A kit comprising, in one or more containers:

(a) about 125 mM to about 175 mM N-butyldiethanolamine (BDEA);

(b) a pH buffering component; and

(c) a salt,

wherein a composition formed from combining components (a), (b), and (c) of the kit has an ionic strength of greater than about 0.3 M.

19. A method for producing a composition comprising combining

(i) about 125 mM to about 175 mM of N-butyldiethanolamine (BDEA);

(ii) a pH buffering component; and

(iii) a salt,

wherein the composition has an ionic strength of greater than about 0.3 M.

20. The composition of claim 1 , wherein the composition has an ionic strength of greater than about 0.3 M.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 29, 2021
From: TUCKER-SCHWARTZ, ALEXANDER K.; SIGAL, GEORGE
To: MESO SCALE TECHNOLOGIES, LLC.
Reel/Frame 055078/0833 →
Continuity (2)
Provisional Application 62787892 · Jan 3, 2019
Related Publication 20220018846A1 · Jan 20, 2022
References Cited (101)
US 5093268A · Leventis et al. · 1992 [cited by applicant]
US 5147806A · Kamin et al. · 1992 [cited by applicant]
US 5238808A · Bard et al. · 1993 [cited by applicant]
US 5240863A · Shibue et al. · 1993 [cited by applicant]
US 5308754A · Kankare et al. · 1994 [cited by applicant]
US 5324457A · Zhang et al. · 1994 [cited by applicant]
US 5589136A · Northrup et al. · 1996 [cited by applicant]
US 5591581A · Massey et al. · 1997 [cited by applicant]
US 5597910A · Gudibande et al. · 1997 [cited by applicant]
US 5641623A · Martin · 1997 [cited by applicant]
US 5643713A · Liang et al. · 1997 [cited by applicant]
US 5679519A · Oprandy · 1997 [cited by applicant]
US 5705402A · Leland et al. · 1998 [cited by applicant]
US 5714089A · Bard et al. · 1998 [cited by applicant]
US 5731147A · Bard et al. · 1998 [cited by applicant]
US 5776672A · Hashimoto et al. · 1998 [cited by applicant]
US 5786141A · Bard et al. · 1998 [cited by applicant]
US 5846485A · Leland et al. · 1998 [cited by applicant]
US 5866434A · Massey et al. · 1999 [cited by applicant]
US 5935779A · Massey et al. · 1999 [cited by applicant]
US 5993740A · Niiyama et al. · 1999 [cited by applicant]
US 6066448A · Wohlstadter et al. · 2000 [cited by applicant]
US 6090545A · Wohlstadter et al. · 2000 [cited by applicant]
US 6136268A · Ala-Kleme et al. · 2000 [cited by applicant]
US 6140045A · Wohlstadter et al. · 2000 [cited by applicant]
US 6207369B1 · Wohlstadter et al. · 2001 [cited by applicant]
US 6316607B1 · Massey et al. · 2001 [cited by applicant]
US 6451225B1 · Leland et al. · 2002 [cited by applicant]
US 6468741B1 · Massey et al. · 2002 [cited by applicant]
US 6479233B1 · Bard et al. · 2002 [cited by applicant]
US 6808939B2 · Sigal et al. · 2004 [cited by applicant]
US 6919173B2 · Tsionsky et al. · 2005 [cited by applicant]
US 6977722B2 · Wohlstadter et al. · 2005 [cited by applicant]
US 7288410B2 · Tsionsky et al. · 2007 [cited by applicant]
US 7491540B2 · Tsionsky et al. · 2009 [cited by applicant]
US 7842246B2 · Wohlstadter et al. · 2010 [cited by applicant]
US 8785201B2 · Tsionsky et al. · 2014 [cited by applicant]
US 9416150B2 · Bergmann et al. · 2016 [cited by applicant]
US 9499573B2 · Bergmann et al. · 2016 [cited by applicant]
US 9618510B2 · Aghvanyan et al. · 2017 [cited by applicant]
US 9731297B2 · Glezer et al. · 2017 [cited by applicant]
US 9891221B2 · Tsionsky et al. · 2018 [cited by applicant]
US 10114015B2 · Glezer et al. · 2018 [cited by applicant]
US 10408823B2 · Aghvanyan et al. · 2019 [cited by applicant]
US 10753934B2 · Tsionsky et al. · 2020 [cited by applicant]
US 20110065135A1 · Daisuke et al. · 2011 [cited by applicant]
US 20120178091A1 · Glezer et al. · 2012 [cited by applicant]
US 20120190586A1 · Waters et al. · 2012 [cited by applicant]
US 20120190589A1 · Anderson et al. · 2012 [cited by applicant]
US 20170168047A1 · Aghvanyan et al. · 2017 [cited by applicant]
US 20180364175A1 · Josel et al. · 2018 [cited by applicant]
US 20190011441A1 · Glezer et al. · 2019 [cited by applicant]
US 20210109100A1 · Tsionsky et al. · 2021 [cited by applicant]
US 20220018785A1 · Tucker-Schwartz et al. · 2022 [cited by applicant]
AU 2008200361A1 · 2008 [cited by applicant]
CN 101532961A · 2009 [cited by applicant]
CN 106124753A · 2016 [cited by applicant]
CN 106526183A · 2017 [cited by applicant]
CN 109444116A · 2019 [cited by applicant]
CN 108698047B · 2022 [cited by applicant]
JP H08502586A · 1996 [cited by applicant]
JP 2001021560A · 2001 [cited by applicant]
WO 1990005296A1 · 1990 [cited by applicant]
WO 1996021154A1 · 1996 [cited by applicant]
WO 1997036931A1 · 1997 [cited by applicant]
WO 1998012539A1 · 1998 [cited by applicant]
WO 1998057154A1 · 1998 [cited by applicant]
WO 1999014599A1 · 1999 [cited by applicant]
WO 1999063347A2 · 1999 [cited by applicant]
WO 1999032662A1 · 1999 [cited by applicant]
WO 1999058962A1 · 1999 [cited by applicant]
WO 2000003233A1 · 2000 [cited by applicant]
WO 2003023380A1 · 2003 [cited by applicant]
WO 2014160192A1 · 2014 [cited by applicant]
WO 2014165061A1 · 2014 [cited by applicant]
WO 2015175856A1 · 2015 [cited by applicant]
WO 2022006236A1 · 2022 [cited by applicant]
Workman, Scott, and Mark M. Richter. “The Effects of Nonionic Surfactants on the Tris(2,2′-bipyridyl)ruthenium(ii)-Tripropylamine Electrochemiluminescence System.” Analytical Chemistry, vol. 72, No. 22, 2000, pp. 5556-5… [cited by examiner]
Chen, Yu et al., “A simple and versatile paper-based electrochemiluminescence biosensing platform for hepatitis B virus surface antigen detection,” Biochemical Engineering Journal 129:1-6 (2017). [cited by applicant]
Debad, Jeff et al., “Clinical and Biological Applications of ECL” in Electrogenerated Chemiluminescence, 1 [cited by applicant]
Han, Shuang et al., “Effect of Hydroxyl and Amino Groups on Electrochemiluminescence Activity of Tertiary Amines at Low tris(2,2′-bipyridyl)ruthenium(II) Concentrations” (Abstract), Talanta 81(1-2):44-47 (2010). [cited by applicant]
International Search Report issued Apr. 22, 2020 in International Appl. No. PCT/US2019/068293. [cited by applicant]
Kirschbaum-Harriman, Stefanie et al., “Improving ruthenium-based ECL through nonionic surfactants and tertiary amines,” Analyst 142(14):2648-2653 (2017). [cited by applicant]
Kirschbaum-Harriman, Stefanie et al., “Signal enhancement and low oxidation potentials for miniaturized ECL biosensors via N-butyldiethanolamine”, Analyst 142(13):2469-2474 (2017). [cited by applicant]
Kitte, Shimeles Addisu et al., “Electrogenerated chemiluminescence of tris(2,2′-bipyridine) ruthenium(II) using N-(3-aminopropyl)diethanolamine as coreactant”, Anal Bioanal Chem 408(25):7059-7065 (2016). [cited by applicant]
Workman et al. “The Effects of Nonionic Surfactants on the Tris(2,2′-bipyridyl)ruthenium(II)-Tripropylamine Electrochemiluminescence System,” Analytical Chemistry, 72(22): 5556-61 (2000). [cited by applicant]
Leland et al., “Electrogenerated Chemiluminescence: An Oxidative-Reduction Type ECL Reaction Sequence Using Tripropyl Amine,” Journal of The Electrochemical Society 137:3127-3131 (1990). [cited by applicant]
Lewis et al., “MSD™ Technology: A New Way to Measure Classic Markers of Apoptosis,” retrieved Mar. 15, 2024, at: https://www.mesoscale.com/˜/media/files/scientific%20poster/apoptosismarkers.pdf. [cited by applicant]
Lorenz, C., et al. Interference assessment of various endogenous and exogenous substances on the performance of the eversense long-term implantable continuous glucose monitoring system. Diabetes Technology & Therapeutic… [cited by applicant]
Perrson, “Implementation of electrochemiluminescence technology for quantification of bioprocess impurities,” Uppsala University School of Engineering, Master's Thesis 1-50 (2008). [cited by applicant]
Rayer et al., “Dissociation Constants (pKa) of Tertiary and Cyclic Amines: Structural and Temperature Dependences,” Journal of Chemical Engineering Data 59(11):3805-3813 (2014). [cited by applicant]
Vagelos, “Malonylsemialdehyde Coenzyme A,” Methods of Enzymatic Analysis 452-453 (1965). [cited by applicant]
Voinescu et al., “Similarity of Salt Influences on the pH of Buffers, Polyelectrolytes, and Proteins,” The Journal of Physical Chemistry B 110(17):8870-8876 (2006). [cited by applicant]
Wu, S., et al. Determining the critical micelle concentrations of surfactants by a simple and fast titration method. Anal. Chem., 92(6):4259-4265 (2020). [cited by applicant]
Zheng et al., “Electrochemiluminescent Behavior of Tris(2,2-bipyridine) Ruthenium(II)/Triethylamine in lonic Liquid Solution,” The Journal of Physical Chemistry C 112(39):15570-75 (2008). [cited by applicant]
Non-Final Office Action issued in U.S. Appl. No. 17/363,758, dated Jan. 16, 2024. [cited by applicant]
International Search Report and Written Opinion in PCT/US2021/039835, dated Dec. 6, 2021. [cited by applicant]
Hu et al., “Applications and trends in electrochemiluminescence,” Chemical Society Reviews, 39: 3275-3304 (2010). [cited by applicant]
Wang et al., “Label free bifunctional electrochemiluminescence aptasensor for detection of adenosine and lysozyme,” Electrochimica Acta, 76: 416-423 (2012). [cited by applicant]
Notice of Allowability in U.S. Appl. No. 17/813,722, United States Patent and Trademark Office, dated Nov. 2, 2023. [cited by applicant]
Notice of Allowability in U.S. Appl. No. 17/813,722, United States Patent and Trademark Office, dated Nov. 15, 2023. [cited by applicant]
Cited By (1)
US 12,429,427