IP Library › Granted Patent US 12,429,427
Granted Patent B2
US 12,429,427 · App. 17/363,758 · Granted Sep 30, 2025

Compositions and methods for assay measurements

Inventors: Alexander K. Tucker-Schwartz (Bethesda, MD); George Sigal (Rockville, MD)
Assignee: MESO SCALE TECHNOLOGIES, LLC.
G01N21/76G01N33/5438G01N33/582G01N2458/30
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Quick Facts
Patent No.
US 12,429,427
App. No.
17/363,758
Granted
Sep 30, 2025
Kind
B2
Abstract

The invention relates to novel compositions comprising an electrochemiluminescence (ECL) co-reactant. In embodiments, the composition further comprises an ionic component, a surfactant, or combination thereof. In embodiments, the ECL co-reactant is triethanolamine (TEA), tert-butyldiethanolamine (tBDEA), methyldiethanolamine (MDEA), 3-[Bis-(2-hydroxy-ethyl)-amino]-propane-1-sulfonic acid (DEA-PS), or a combination thereof. Methods of using the compositions and kits comprising the compositions are also provided herein.

Claims (21)

1. A composition comprising:

a) 1000 mM to 6500 mM of triethanolamine (TEA);

b) an ionic component; and

c) an electrochemiluminescence (ECL)-labeled component;

wherein the composition has a pH of 7 to 8.

2. The composition of claim 1 , wherein the concentration of the TEA is 1200 mM to 1600 mM.

3. The composition of claim 1 , wherein the concentration of the ionic component is 500 mM to 2000 mM.

4. The composition of claim 1 , wherein the ionic component comprises a chloride ion.

5. The composition of claim 4 , wherein the ionic component comprises NaCl, KCl, LiCl, or combination thereof.

6. The composition of claim 5 , wherein the ionic component comprises NaCl.

7. The composition of claim 1 , wherein the ECL-labeled component comprises a detection reagent that comprises an ECL label; or wherein the ECL-labeled component comprises a binding partner of a detection reagent, wherein the binding partner comprises an ECL label.

8. The composition of claim 7 , wherein the ECL-labeled component is a detection reagent that comprises an ECL label.

9. The composition of claim 7 , wherein the ECL label comprises an electrochemiluminescent organometallic complex.

10. The composition of claim 1 , wherein the composition further comprises a surfactant.

11. The composition of claim 10 , wherein the concentration of the surfactant is 0.1 mM to 10 mM.

12. The composition of claim 10 , wherein the surfactant is a non-ionic surfactant.

13. The composition of claim 12 , wherein the non-ionic surfactant is an alkyl ether-polyethylene glycol (PEG).

14. The composition of claim 1 , wherein the composition has a pH of 7.9 to 8.

15. The composition of claim 1 , wherein the composition has a pH of 7.9.

16. The composition of claim 1 , wherein the composition is substantially free of an additional pH buffering component.

17. The composition of claim 1 , wherein the composition does not comprise any of phosphate, Tris, HEPES, glycylglycine, borate, acetate, and citrate.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 28, 2021
From: TUCKER-SCHWARTZ, ALEXANDER K.; SIGAL, GEORGE
To: MESO SCALE TECHNOLOGIES, LLC.
Reel/Frame 057951/0879 →
Continuity (2)
Provisional Application 63047167 · Jul 1, 2020
Related Publication 20220018785A1 · Jan 20, 2022
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