Granted Patent B2

US 12,208,381 · App. 17/151,073 · Granted Jan 28, 2025

Inventors: Benjamin Förthmann (Hamburg, DE); Philipp Cloer (Hamburg, DE); Jens Kleemann (Hamburg, DE); Torsten Krauss (Hamburg, DE)

Assignee: EPPENDORF SE

B01L3/0237B01L3/0217B01L2200/143B01L2300/023B01L2300/024

Abstract

Claims (54)

1. Method ( 100 ) for operating a hand-held, computer-controlled piston-stroke pipette ( 1 ) used for the computer-controlled execution of a pipetting operation with a fluid sample and for the automatic prewetting of the inside of a pipette tip ( 10 ), arranged at the nose cone ( 11 ) of the piston-stroke pipette, comprising the computer-controlled steps, which are performed in the following order

Providing a function nvb(x) specifying a nvb of one or more prewetting steps as a function of a variable x characterizing the pipetting operation, ( 101 ) the function nvb(x) comprising a data assignment table for assigning the number nvb to the variable x,

wherein the variable x contains at least one of the parameter values of the following parameters or is formed by it or by parameters that can be determined from the parameters selected from the group consisting of: a parameter ID_LM chemically identifying the main liquid component of a fluid sample to be pipetted, a parameter ID_VM identifying a diluent contained in a fluid sample to be pipetted, a parameter ID_GT identifying the type of device of the piston-stroke pipette executing the pipetting operation, a parameter V_nom identifying the type of device of the piston-stroke pipette executing the pipetting operation by its nominal volume, a parameter ID_ST identifying the type of pipette tip of the pipette tip used in the pipetting operation, a speed v_K of a piston-stroke of the piston-stroke pipette executing during the pipetting operation or during at least one prewetting step, a temperature T of the surrounding of the piston-stroke pipette or the fluid sample to be pipetted in the pipetting operation at a time of the pipetting operation, an air pressure or a vapor pressure P of the surrounding of the piston-stroke pipette at a time of the pipetting operation;

Acquiring of the at least one parameter value of the variable x characterizing the pipetting operation; ( 102 )

Determining the number nvb of prewetting step associated to the variable x by means of the function nvb(x); ( 103 )

Executing a sequence of the number nvb of prewetting steps, ( 104 )

in which nvb>0 and in which the prewetting step in each case comprises that the piston-stroke pipette executes an electrically driven piston movement in order to take up a sample volume into the pipette tip and that subsequently an inverse piston movement is executed in order to release the sample volume contained in the pipette tip from the pipette tip at least partially or completely.

2. Method according to claim 1 , wherein the variable x contains the parameter value V of the volume of the pipetting volume V to be aspirated into the pipette tip in the pipetting operation or is formed by this parameter value V.

3. Method according to claim 1 , wherein the function nvb(x) comprises at least one calculation algorithm in order to assign the number nvb to the variable x.

4. Method according to claim 1 , wherein the function nvb(x) optimizes a number nvb of one or several prewetting steps as a function of a variable x characterizing the pipetting operation such that the air pressure in the air cushion between the fluid sample and the motionless piston of the piston-stroke pipette achieved by means of the prewettings is sufficiently constant for avoiding the dripping of the sample aspirated into the pipette tip in the pipetting operation.

5. Method according to claim 1 , wherein the variable x contains the parameter value V of the volume of the pipetting volume V to be aspirated into the pipetting tip in the pipetting operation or is formed by this parameter value V, and wherein the function nvb(V) is described as a linear relation between nvb and V, thus nvb=a*V+b, with a and b being real numbers, wherein the range of the volumes V that can be pipetted is preferentially divided in two section, in each of which a characteristic set of parameters a, b is valid, so that the relation nvb=a 1 **V+b 1 is valid in the range V 1 to V 2 of the possible volumes and the relation nvb=a 2 *V+b 2 is valid in the range V 2 to V 3 of the possible volumes, and a 1 *< >a 2 and b 1 < >b 2 are valid.

6. Method for executing a computer-controlled pipetting operation by means of a hand-held computer-controlled piston-stroke pipette, comprising the method according to claim 1 , and comprising the step to automatically execute the following computer-controlled step subsequent to the execution of the sequence of the number n of one or more prewetting steps: Aspirating a sample volume V of the fluid sample into the pipette tip and, holding that sample volume V of the fluid sample in the pipette tip ( 205 ).

7. Method according to claim 1 , wherein an external data processing device is provided, which comprises a user interface device and an electronic control device, and wherein the piston-stroke pipette is provided or several piston-stroke pipettes are provided, wherein each piston-stroke pipette comprises an electronic control device, wherein the control devices of the external data processing device and of the piston-stroke pipette are configured to exchange data among each other via a data connection,

wherein the control device of the external data processing device is configured to acquire at least one or all of the said parameters of the variable x by means of the user interface device.

8. Method according to claim 7 , wherein the control device of the external data processing device is configured to determine by means of the function nvb(x) the value of the number nvb of prewetting steps from at least one or all of the said parameters of the variable x.

9. Method according to claim 7 , wherein the external data processing device and/or the at least one piston-stroke pipette comprises a non-transitory data storage medium, in which the function nvb(x) is stored and/or the value nvb can be stored.

10. Method according to claim 9 , wherein the control device of the at least one piston-stroke pipette acquires the value nvb via the data connection and stores it in a non-transitory data storage medium of the piston-stroke pipette.

11. System ( 200 ) for the automated prewetting of the inside of a pipette tip arranged at the nose cone of a hand-held, computer-controlled piston-stroke pipette that serves for the computer-controlled execution of a pipetting operation with a fluid sample, comprising

a hand-held piston-stroke pipette ( 1 ) for the computer-controlled execution of a pipetting operation with a fluid sample, comprising

an electronic control device,

a piston chamber and a piston that can move therein,

an electric piston drive for moving the piston,

a nose cone, to which a pipette tip can be attached,

wherein the electronic control device is configured to control the piston drive and to execute a pipetting program comprising the following steps

execution of a sequence of a number nvb of one or several prewetting steps,

wherein each prewetting step comprises that an electrically driven piston-stroke is executed by the piston-stroke pipette in order to take up a sample volume into the pipette tip and that subsequently an inverse piston-stroke is executed in order to release the sample volume at least partially or completely from the pipette tip,

subsequent to the at least one prewetting step

executing a pipetting operation comprising the aspiration of a sample volume V of the fluid sample into the pipette tip and holding of that sample volume V of the fluid sample in the pipette tip;

an external data processing device ( 21 ) comprising a data interface device and an electronic control device,

wherein the control devices of the external data processing device and of the piston-stroke pipette are configured to exchange data with each other via a data connection,

wherein the control device of the external data processing device is configured to acquire a variable x by means of the data interface device, and

the system is configured to determine by means of the function nvb(x) the value of the number nvb of prewetting steps from at least one or all of the said parameters of the variable x, wherein the electronic control device is configured to execute the following steps

utilizing a function nvb (x) specifying a number nvb of one or more prewetting steps as a function of a variable x characterizing the pipetting operation, ( 101 );

the function nvb(x) comprising a data assignment table for assigning the number nvb to the variable x,

wherein the variable x contains at least one of the parameter values of the following parameters or is formed by it or by parameters that can be determined from the parameters listed in the following: a parameter ID_LM chemically identifying the main liquid component of the fluid sample to be pipetted, a parameter ID_VM identifying a diluent contained in the fluid sample to be pipetted, a parameter ID_GT identifying the type of device of the piston-stroke pipetted executing the pipetting operation, a parameter V_nom identifying the type of device of the piston-stroke pipette executing the pipetting operation by its nominal volume, a parameter ID_ST identifying the type of pipette tip of the pipette tip used in the pipetting operation, a speed v_K of the piston-stroke of the piston-stroke pipette executed during this pipetting operation or during at least one prewetting step, a temperature T of the surroundings of the piston-stroke pipette or the fluid sample to be pipetted in the pipetting operation at the time of pipetting operation, an air pressure or a vapor pressure P of the surroundings of the piston-stroke pipette at the time of the pipetting operation;

acquiring the at least one parameter value of the variable x characterizing the pipetting operation; ( 102 ) determining the number nvb of prewetting step associated to the variable x by means of the function nvb(x); ( 103 );

wherein the control device of the at least one piston-stroke pipette is configured to acquire the number nvb of prewetting steps via the data connection.

12. Computer program for operating a hand-held, computer-controlled piston-stroke pipette, that serves for the computer-controlled execution of a pipetting operation with the fluid sample and for the automated prewetting of the inside of a pipette tip that is arranged at the nose cone of the piston-stroke pipette, wherein the computer program comprises commands which, when the computer program is executed by the central processing unit of at least one electrical control device, causing the central processing unit to execute the following steps

Acquiring of the at least one parameter value of the variable x characterizing the pipetting operation;

Accessing a non-transitory data storage medium in which a function nvb(x) is stored that indicates a number nvb of one or more prewetting steps as a function of a variable x characterizing the pipetting operation,

Determining the number nvb of prewetting step associated to the variable x by means of the function nvb(x);

Providing at least the value nvb to the data processing of the control device of the piston-stroke pipette and transferring at least the value nvb to the control device of the piston-stroke pipette.

13. Data processing device comprising

a data interface device, and

an electronic control device,

wherein the control device of the data processing device is configured to exchange, via a data connection, data with the control device of a hand-held piston-stroke pipette, which serves for the computer-controlled execution of a pipetting operation with a fluid sample,

wherein the electronic control device of the data processing device is configured to acquire at least one parameter of a variable x by means of the data interface device, and

wherein the electronic control device of the data processing device is configured to determine the value of the number nvb of the prewetting steps from the at least one or all of the said parameters of the variable x by executing the following steps in order

utilizing a function nvb(x) specifying a number nvb of one or more prewetting steps as a function of a variable x characterizing the pipetting operation, ( 101 );

the function nvb(x) comprising a data assignment table for assigning the number nvb to the variable x,

wherein the variable x contains at least one of the parameter values of the following parameters or is formed by it or by parameters that can be determined from the parameters listed in the following: a parameter ID_LM chemically identifying the main liquid component of the fluid sample to be pipetted, a parameter ID_VM identifying a diluent contained in the fluid sample to be pipetted, a parameter ID_GT identifying the type of device of the piston-stroke pipetted executing the pipetting operation, a parameter V_nom identifying the type of device of the piston-stroke pipette executing the pipetting operation by its nominal volume, a parameter ID_ST identifying the type of pipette tip of the pipette tip used in the pipetting operation, a speed v_K of the piston-stroke of the piston-stroke pipette executed during this pipetting operation or during at least one prewetting step, a temperature T of the surroundings of the piston-stroke pipette or the fluid sample to be pipetted in the pipetting operation at the time of pipetting operation, an air pressure or a vapor pressure P of the surroundings of the piston-stroke pipette at the time of the pipetting operation;

acquiring the at least one parameter value of the variable x characterizing the pipetting operation; ( 102 )

determining the number nvb of prewetting step associated to the variable x by means of the function nvb(x); ( 103 ); and

wherein the electronic control device of the data processing device is configured to provide it to the data processing of the electronic control device of the piston-stroke pipette and/or to transfer it to the piston-stroke pipette via the data connection.

Assignments (1)

Continuity (1)

References Cited (44)