Surface functionalized, host-guest polymer nano-assemblies and methods thereof
The invention generally relates to polymer-based nano-structures. More particularly, the invention relates to novel, surface-functionalized, guest-host polymer nano-assemblies and nano-delivery vehicles useful in diverse fields including drug delivery, diagnostics and specialty materials. The nano-assemblies and nano-delivery vehicles of the invention are afforded via simplify and reliable approaches.
1. A polymer-based nanoparticle having a vesicular structure stabilized by intraparticular crosslinking inside the vesicular structure, wherein the polymer comprises the monomer unit:
wherein Z 3 is
wherein Z′ is selected from O or NH;
R 3 is selected from H, C 1 -C 12 alkyl group, or halogen;
R L3 is CR 4 , wherein R 4 is H, C 1 -C 12 alkyl group or halogen;
R F comprises C(O)(NH)CH 2 CH 2 NH 2 or C(O)NH(oligoethylene glycol);
R x is a crosslinking group capable of inter- or intra-molecular crosslinking or an inter- or intra-molecularly crosslinked group, selected from pyridinyl disulfide and thiol; and
r is an integer from about 10 to about 1000.
2. The polymer-based nanoparticle of claim 1 , wherein R F comprises C(O) NHCH 2 CH 2 NH 2 .
3. The polymer-based nanoparticle of claim 2 , wherein R x comprises pyridinyl disulfide.
4. The polymer-based nanoparticle of claim 2 , wherein R x comprises thiol.
5. The polymer-based nanoparticle of claim 1 , wherein R F comprises a C(O)NH (oligoethylene glycol) group.
6. The polymer-based nanoparticle of claim 5 , wherein R x comprises pyridinyl disulfide.
7. The polymer-based nanoparticle of claim 5 , wherein R x comprises thiol.
8. The polymer-based nanoparticle of claim 6 , wherein Z′ is NH, R 3 is H, and R L3 is CH.
9. The polymer-based nanoparticle of claim 1 , wherein the nanoparticle is surface functionalized by a peptide, a nucleic acid, or a molecular ligand.
10. A nano-assembly comprising:
a host crosslinked polymer-based nanoparticle having a vesicular structure according to claim 1 ; and
a hydrophilic guest molecular cargo non-covalently encapsulated in the host crosslinked polymer-based nanoparticle,
wherein the host crosslinked polymer is addressable by a biological or chemical intervention resulting in partial or complete decrosslinking of the host polymer and release of the hydrophilic guest molecular cargo from the nano-assembly.
11. The nano-assembly of claim 10 , wherein the hydrophilic guest molecular cargo is a therapeutic agent, a diagnostic agent, or an imaging agent.
12. The nano-assembly of claim 10 , wherein the hydrophilic guest molecular cargo is a hydrophilic small molecule, a peptide or a nucleic acid.
13. The nano-assembly of claim 12 , wherein the hydrophilic guest molecular cargo is a protein or an enzyme.
14. The nano-assembly of claim 11 , wherein Z′ is NH, R 3 ; is H, R L3 is CH, R F comprises C(O)NH (oligoethylene glycol), and R x comprises pyridinyl disulfide.