IP Library › Granted Patent US 12,241,891
Granted Patent B2
US 12,241,891 · App. 17/675,154 · Granted Mar 4, 2025

Multivalent binding composition for nucleic acid analysis

Inventors: Michael Previte (San Diego, CA); Molly He (San Diego, CA); Junhua Zhao (San Diego, CA); Hui Zhen Mah (San Diego, CA); Chunhong Zhou (San Diego, CA); Sinan Arslan (San Diego, CA); Matthew Kellinger (San Diego, CA); Lorenzo Berti (San Diego, CA); Steve Xiangling Chen (San Diego, CA)
Assignee: ELEMENT BIOSCIENCES, INC.
G01N33/5308C12Q1/6874G01N33/582
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Quick Facts
Patent No.
US 12,241,891
App. No.
17/675,154
Granted
Mar 4, 2025
Kind
B2
Abstract

Multivalent binding compositions including a particle-nucleotide conjugate having a plurality of copies of a nucleotide attached to the particle are described. The multivalent binding compositions allow one to localize detectable signals to active regions of biochemical interaction, e.g., sites of protein-protein interaction, protein-nucleic acid interaction, nucleic acid hybridization, or enzymatic reaction, and can be used to identify sites of base incorporation in elongating nucleic acid chains during polymerase reactions and to provide improved base discrimination for sequencing and array based applications.

Claims (26)

1. A method for identifying at least a portion of a sub-cellular component within a cell or tissue in situ, the method comprising:

(a) detecting a signal from a binding complex between said sub-cellular component and a detectable polymer-nucleotide conjugate within said cell or said tissue, wherein said detectable polymer-nucleotide conjugate comprises at least two nucleotides that are transiently interacting with at least two of said sub-cellular components; and

(b) processing at least said signal detected in (a) to identify said at least said portion of said sub-cellular component.

2. The method of claim 1 , wherein said sub-cellular component is a protein.

3. The method of claim 1 , further comprising: (c) immobilizing said cell or said tissue on a surface of a substrate.

4. The method of claim 3 , further comprising: (d) coupling at least a portion of said sub-cellular component to a capture molecule coupled to said surface.

5. The method of claim 1 , further comprising permeabilizing said tissue or lysing said cell prior to said detecting in (a).

6. The method of claim 3 , wherein said surface has a water contact angle of less than or equal to 45 degrees.

7. The method of claim 4 , wherein said coupling in (d) comprises hybridizing said capture molecule with said at least said portion of said sub-cellular component in a presence of a hybridization buffer comprising:

(a) a first polar aprotic solvent having a dielectric constant that is no greater than 40 and having a polarity index of 4-9; and

(b) a second polar aprotic solvent having a dielectric constant that is less than or equal to 115.

8. The method of claim 3 , wherein an image of said surface exhibits a contrast-to-noise ratio of greater than or equal to 5 as measured by:

(a) contacting said surface with a fluorescently labeled nucleotide molecule comprising a nucleic acid sequence that is complementary to at least a portion of a capture oligonucleotide immobilized to said surface; and

(b) following (a), imaging said surface with an inverted microscope and a camera under non-signal saturating conditions while said surface is immersed in a buffer.

9. The method of claim 1 , wherein detecting said signal from said binding complex in (a) comprises: performing a nucleotide binding reaction between a nucleotide moiety coupled to said detectable polymer-nucleotide conjugate and said sub-cellular component, wherein said detectable polymer-nucleotide conjugate comprises one or more detectable moieties.

10. The method of claim 1 , wherein said detectable polymer-nucleotide conjugate comprises: two or more nucleotide moieties attached to a polymer core, wherein said detectable polymer-nucleotide conjugate is configured to form said binding complex between said two or more nucleotide moieties and said sub-cellular component.

11. The method of claim 3 , further comprising: (d) determining an origin of said at least said portion of said sub-cellular component in said cell or said tissue, wherein said origin comprises a cell type or a tissue type.

12. The method of claim 1 , wherein (a) and (b) are performed with accuracy of base-calling that is characterized by a Q-score of greater than 25 for at least 80% of nucleotides identified.

13. The method of claim 1 , wherein said sub-cellular component is a nucleic acid sequence.

14. The method of claim 13 , wherein said nucleic acid sequence is a deoxyribonucleic acid (DNA) sequence.

15. The method of claim 13 , wherein said signal detected in (a) is from a detectable moiety coupled to said binding complex, and wherein said signal is indicative of an identity of a nucleotide in said nucleic acid sequence.

16. The method of claim 11 , wherein said determining in (d) is performed, at least in part, by analyzing a relative three-dimensional relationship between said sub-cellular component and a point of reference of said cell or said tissue.

17. The method of claim 16 , wherein said immobilizing in (c) comprises immobilizing said cell or said tissue on said surface in a manner that is sufficient to fix said relative three-dimensional relationship.

18. The method of claim 4 , wherein said sub-cellular component is a nucleic acid sequence.

19. The method of claim 18 , further comprising amplifying said nucleic acid sequence on said surface of said substrate, wherein said amplifying comprises performing a rolling circle amplification reaction.

20. The method of claim 19 , wherein said signal detected in (a) is from a detectable moiety coupled to said binding complex, and wherein said signal is indicative of an identity of a nucleotide in said nucleic acid sequence.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 9, 2022
From: PREVITE, MICHAEL; HE, MOLLY; ZHAO, JUNHUA; ARSLAN, SINAN; KELLINGER, MATTHEW; BERTI, LORENZO; MAH, HUI ZHEN; CHEN, STEVE XIANGLING; ZHOU, CHUNHONG
To: ELEMENT BIOSCIENCES, INC.
Reel/Frame 059209/0779 →
Continuity (4)
Continuation 17356929 · Jun 24, 2021
Continuation PCTUS2020052305 · Sep 23, 2020
Provisional Application 62904623 · Sep 23, 2019
Related Publication 20220170919A1 · Jun 2, 2022
References Cited (290)
US 3950649A · Yonekubo · 1976 [cited by applicant]
US 4222743A · Wang · 1980 [cited by applicant]
US 5184021A · Smith · 1993 [cited by applicant]
US 5422712A · Ogino · 1995 [cited by applicant]
US 5512131A · Kumar et al. · 1996 [cited by applicant]
US 5558991A · Trainor · 1996 [cited by applicant]
US 5695936A · Mandrand et al. · 1997 [cited by applicant]
US 6287766B1 · Nolan et al. · 2001 [cited by applicant]
US 6440748B1 · Katerkamp et al. · 2002 [cited by applicant]
US 6482590B1 · Ullman et al. · 2002 [cited by applicant]
US 6548607B2 · Halverson et al. · 2003 [cited by applicant]
US 6664079B2 · Ju et al. · 2003 [cited by applicant]
US 6818425B2 · Hjorleifsdottir et al. · 2004 [cited by applicant]
US 6829051B2 · Abe et al. · 2004 [cited by applicant]
US 6833246B2 · Balasubramanian · 2004 [cited by applicant]
US 7030383B2 · Babayoff et al. · 2006 [cited by applicant]
US 7169560B2 · Lapidus et al. · 2007 [cited by applicant]
US 7211390B2 · Rothberg et al. · 2007 [cited by applicant]
US 7244559B2 · Rothberg et al. · 2007 [cited by applicant]
US 7264929B2 · Rothberg et al. · 2007 [cited by applicant]
US 7264934B2 · Fuller · 2007 [cited by applicant]
US 7416844B2 · Korlach et al. · 2008 [cited by applicant]
US 7566537B2 · Balasubramanian et al. · 2009 [cited by applicant]
US 7755841B2 · Christenson et al. · 2010 [cited by applicant]
US 7960116B2 · Eid et al. · 2011 [cited by applicant]
US 8039817B2 · Feng et al. · 2011 [cited by applicant]
US 8120002B2 · Van Dijk et al. · 2012 [cited by applicant]
US 8133672B2 · Bjornson et al. · 2012 [cited by applicant]
US 8143599B2 · Feng et al. · 2012 [cited by applicant]
US 8242463B2 · Feng et al. · 2012 [cited by applicant]
US 8257954B2 · Clark et al. · 2012 [cited by applicant]
US 8278630B1 · Feng et al. · 2012 [cited by applicant]
US 8367813B2 · Korlach · 2013 [cited by applicant]
US 8399196B2 · Hoser · 2013 [cited by applicant]
US 8405048B2 · Hayashi · 2013 [cited by applicant]
US 8481258B2 · Church et al. · 2013 [cited by applicant]
US 8481264B2 · Bjornson et al. · 2013 [cited by applicant]
US 8530164B2 · Patel et al. · 2013 [cited by applicant]
US 8546772B2 · Feng et al. · 2013 [cited by applicant]
US 8580539B2 · Korlach · 2013 [cited by applicant]
US 8586947B1 · Feng et al. · 2013 [cited by applicant]
US 8592148B2 · Williams et al. · 2013 [cited by applicant]
US 8632975B2 · Vander Horn et al. · 2014 [cited by applicant]
US 8637650B2 · Cherkasov et al. · 2014 [cited by applicant]
US 8658365B2 · Bjornson et al. · 2014 [cited by applicant]
US 8698102B2 · Feng et al. · 2014 [cited by applicant]
US 8703461B2 · Peris et al. · 2014 [cited by applicant]
US 8715932B2 · Su et al. · 2014 [cited by applicant]
US 8741566B2 · Winther et al. · 2014 [cited by applicant]
US 9068220B2 · Feng et al. · 2015 [cited by applicant]
US 9255258B2 · Vander Horn et al. · 2016 [cited by applicant]
US 9273349B2 · Nguyen et al. · 2016 [cited by applicant]
US 9279154B2 · Previte et al. · 2016 [cited by applicant]
US 9365898B2 · Feng et al. · 2016 [cited by applicant]
US 9399767B2 · Peris et al. · 2016 [cited by applicant]
US 9546398B2 · Peter et al. · 2017 [cited by applicant]
US 9593315B2 · Peris et al. · 2017 [cited by applicant]
US 9605310B2 · Balasubramanian et al. · 2017 [cited by applicant]
US 9765310B2 · Vander Horn et al. · 2017 [cited by applicant]
US 9932631B1 · Dambacher et al. · 2018 [cited by applicant]
US 9951385B1 · Vijayan et al. · 2018 [cited by applicant]
US 9957291B2 · Sebo et al. · 2018 [cited by applicant]
US 10077470B2 · Vijayan et al. · 2018 [cited by applicant]
US 10233490B2 · Stapleton et al. · 2019 [cited by applicant]
US 10246744B2 · Vijayan et al. · 2019 [cited by applicant]
US 10253352B2 · Nguyen et al. · 2019 [cited by applicant]
US 10294514B2 · Iyidogan et al. · 2019 [cited by applicant]
US 10300452B2 · Sun et al. · 2019 [cited by applicant]
US 10301622B2 · Mirkin et al. · 2019 [cited by applicant]
US 10309879B2 · Chen et al. · 2019 [cited by applicant]
US 10336991B2 · Peris et al. · 2019 [cited by applicant]
US 10400272B1 · Middleton et al. · 2019 [cited by applicant]
US 10400275B2 · Aurich-Costa · 2019 [cited by applicant]
US 10415029B2 · Dambacher et al. · 2019 [cited by applicant]
US 10428378B2 · Iyidogan et al. · 2019 [cited by applicant]
US 10443098B2 · Vijayan et al. · 2019 [cited by applicant]
US 10501796B2 · Buermann et al. · 2019 [cited by applicant]
US 10519496B2 · Balasubramanian et al. · 2019 [cited by applicant]
US 10584379B2 · Vijayan et al. · 2020 [cited by applicant]
US 10597643B2 · Iyidogan et al. · 2020 [cited by applicant]
US 10655176B2 · Stromberg et al. · 2020 [cited by applicant]
US 10704094B1 · Arslan et al. · 2020 [cited by applicant]
US 10731141B2 · Iyidogan · 2020 [cited by applicant]
US 10768173B1 · Arslan et al. · 2020 [cited by applicant]
US 10787573B2 · Zheng et al. · 2020 [cited by applicant]
US 10876148B2 · Zhou et al. · 2020 [cited by applicant]
US 10919033B2 · Ren et al. · 2021 [cited by applicant]
US 10961566B2 · Chee · 2021 [cited by applicant]
US 10982280B2 · Arslan et al. · 2021 [cited by applicant]
US 11053540B1 · Chen et al. · 2021 [cited by applicant]
US 11060138B1 · Chen et al. · 2021 [cited by applicant]
US 11118214B2 · Matthiesen et al. · 2021 [cited by applicant]
US 11118220B2 · Daugharthy et al. · 2021 [cited by applicant]
US 11198121B1 · Guo et al. · 2021 [cited by applicant]
US 11200446B1 · Zhou et al. · 2021 [cited by applicant]
US 11220707B1 · Arslan et al. · 2022 [cited by applicant]
US 11236388B1 · Arslan et al. · 2022 [cited by applicant]
US 11261489B2 · Chen et al. · 2022 [cited by applicant]
US 11287422B2 · Previte et al. · 2022 [cited by applicant]
US 11339433B2 · Chen et al. · 2022 [cited by applicant]
US 11427855B1 · Arslan et al. · 2022 [cited by applicant]
US 11535892B1 · Arslan et al. · 2022 [cited by applicant]
US 11781185B2 · Arslan et al. · 2023 [cited by applicant]
US 11795504B2 · Chen et al. · 2023 [cited by applicant]
US 20010031483A1 · Sorge et al. · 2001 [cited by applicant]
US 20020030811A1 · Schindler · 2002 [cited by applicant]
US 20020119455A1 · Chan · 2002 [cited by applicant]
US 20020139936A1 · Dumas · 2002 [cited by applicant]
US 20030152490A1 · Trulson et al. · 2003 [cited by applicant]
US 20050064435A1 · Su et al. · 2005 [cited by applicant]
US 20060286570A1 · Rowlen et al. · 2006 [cited by applicant]
US 20070009954A1 · Wang et al. · 2007 [cited by applicant]
US 20070031829A1 · Yasuno et al. · 2007 [cited by applicant]
US 20070042400A1 · Choi et al. · 2007 [cited by applicant]
US 20070042419A1 · Barany et al. · 2007 [cited by applicant]
US 20080065609A1 · Jenkins et al. · 2008 [cited by applicant]
US 20080160580A1 · Adessi et al. · 2008 [cited by applicant]
US 20080219888A1 · Lawson et al. · 2008 [cited by applicant]
US 20090186343A1 · Wang et al. · 2009 [cited by applicant]
US 20090286691A1 · Kim et al. · 2009 [cited by applicant]
US 20100093992A1 · Cherkasov et al. · 2010 [cited by applicant]
US 20100137143A1 · Rothberg et al. · 2010 [cited by applicant]
US 20100233696A1 · Joseph et al. · 2010 [cited by applicant]
US 20100311144A1 · Peris et al. · 2010 [cited by applicant]
US 20100330570A1 · Vander Horn et al. · 2010 [cited by applicant]
US 20110039259A1 · Ju et al. · 2011 [cited by applicant]
US 20110111975A1 · Schneider et al. · 2011 [cited by applicant]
US 20110301044A1 · Feng et al. · 2011 [cited by applicant]
US 20120014977A1 · Furihata et al. · 2012 [cited by applicant]
US 20120165219A1 · Van Der Zaag et al. · 2012 [cited by applicant]
US 20120231972A1 · Golyshin et al. · 2012 [cited by applicant]
US 20120252012A1 · Armougom et al. · 2012 [cited by applicant]
US 20120253689A1 · Rogan · 2012 [cited by applicant]
US 20120322666A1 · Pham et al. · 2012 [cited by applicant]
US 20130171631A1 · Becker et al. · 2013 [cited by applicant]
US 20130225623A1 · Buxbaum et al. · 2013 [cited by applicant]
US 20140113839A1 · Wu et al. · 2014 [cited by applicant]
US 20150362458A1 · Yanagawa et al. · 2015 [cited by applicant]
US 20160076023A1 · Quake et al. · 2016 [cited by applicant]
US 20160083786A1 · Liu et al. · 2016 [cited by applicant]
US 20160357173A1 · Foschini et al. · 2016 [cited by applicant]
US 20170145495A1 · Sebo et al. · 2017 [cited by applicant]
US 20170145496A1 · Sebo et al. · 2017 [cited by applicant]
US 20170159136A1 · Church et al. · 2017 [cited by applicant]
US 20170189444A1 · Ismagilov et al. · 2017 [cited by applicant]
US 20170191125A1 · Vijayan et al. · 2017 [cited by applicant]
US 20170362649A1 · Lieberman-Aiden et al. · 2017 [cited by applicant]
US 20170369857A1 · Vander Horn et al. · 2017 [cited by applicant]
US 20180023108A1 · Chen et al. · 2018 [cited by applicant]
US 20180080073A1 · Vijayan et al. · 2018 [cited by applicant]
US 20180187245A1 · Dambacher et al. · 2018 [cited by applicant]
US 20180195099A1 · Kranz et al. · 2018 [cited by applicant]
US 20180208983A1 · Dambacher et al. · 2018 [cited by applicant]
US 20180237847A1 · Culler et al. · 2018 [cited by applicant]
US 20180251825A1 · Stoeckius et al. · 2018 [cited by applicant]
US 20180280975A1 · Kilcoin et al. · 2018 [cited by applicant]
US 20180346507A1 · Sebo et al. · 2018 [cited by applicant]
US 20180360974A1 · Kwiatkowski et al. · 2018 [cited by applicant]
US 20190048404A1 · Dambacher · 2019 [cited by applicant]
US 20190119740A1 · Ahn et al. · 2019 [cited by applicant]
US 20190119742A1 · Zhang et al. · 2019 [cited by applicant]
US 20190241945A1 · Malyshev et al. · 2019 [cited by applicant]
US 20190276884A1 · Stapleton et al. · 2019 [cited by applicant]
US 20190338352A1 · Nemiroski et al. · 2019 [cited by applicant]
US 20190367974A1 · Fleischer et al. · 2019 [cited by applicant]
US 20200010885A1 · Malyshev et al. · 2020 [cited by applicant]
US 20200032317A1 · Rohrman et al. · 2020 [cited by applicant]
US 20200087637A1 · Iyidogan · 2020 [cited by applicant]
US 20200149095A1 · Arslan et al. · 2020 [cited by applicant]
US 20200179921A1 · Arslan et al. · 2020 [cited by applicant]
US 20200182866A1 · Arslan et al. · 2020 [cited by applicant]
US 20200347443A1 · Arslan et al. · 2020 [cited by applicant]
US 20200370113A1 · Kellinger et al. · 2020 [cited by applicant]
US 20210040534A1 · Zhou et al. · 2021 [cited by applicant]
US 20210072234A1 · Arslan et al. · 2021 [cited by applicant]
US 20210121882A1 · Guo et al. · 2021 [cited by applicant]
US 20210123098A1 · Previte et al. · 2021 [cited by applicant]
US 20210123911A1 · Arslan et al. · 2021 [cited by applicant]
US 20210139884A1 · Kellinger et al. · 2021 [cited by applicant]
US 20210139981A1 · Arslan et al. · 2021 [cited by applicant]
US 20210223161A1 · Chen et al. · 2021 [cited by applicant]
US 20210247389A1 · Arslan et al. · 2021 [cited by applicant]
US 20210269793A1 · Kellinger et al. · 2021 [cited by applicant]
US 20210318295A1 · Arslan et al. · 2021 [cited by applicant]
US 20210332416A1 · Chen et al. · 2021 [cited by applicant]
US 20210332430A1 · Arslan et al. · 2021 [cited by applicant]
US 20210333211A1 · Chen et al. · 2021 [cited by applicant]
US 20210373000A1 · Arslan et al. · 2021 [cited by applicant]
US 20210387184A1 · Guo et al. · 2021 [cited by applicant]
US 20220136047A1 · Chen et al. · 2022 [cited by applicant]
US 20220186310A1 · Arslan et al. · 2022 [cited by applicant]
US 20220251643A1 · Chen et al. · 2022 [cited by applicant]
US 20220251644A1 · Chen et al. · 2022 [cited by applicant]
US 20220267842A1 · Chen et al. · 2022 [cited by applicant]
US 20220275437A1 · Stapleton et al. · 2022 [cited by applicant]
US 20220290216A1 · Middleton et al. · 2022 [cited by applicant]
US 20220389408A1 · Ben-Yehezkel · 2022 [cited by applicant]
US 20230167434A1 · Kellinger et al. · 2023 [cited by applicant]
US 20230235392A1 · Arslan et al. · 2023 [cited by applicant]
US 20230295692A1 · Berti et al. · 2023 [cited by applicant]
US 20230296592A1 · Previte et al. · 2023 [cited by applicant]
US 20230296593A1 · Previte et al. · 2023 [cited by applicant]
US 20230323450A1 · Arslan et al. · 2023 [cited by applicant]
US 20240117428A1 · Previte et al. · 2024 [cited by applicant]
US 20240200133A1 · Ghorbani et al. · 2024 [cited by applicant]
US 20240201088A1 · Ghorbani et al. · 2024 [cited by applicant]
US 20240230631A1 · Arslan et al. · 2024 [cited by applicant]
EP 3947731A1 · 2022 [cited by applicant]
WO WO9001069A1 · 1990 [cited by applicant]
WO WO2005111240A2 · 2005 [cited by applicant]
WO WO2006065266A2 · 2006 [cited by applicant]
WO WO2006084132A2 · 2006 [cited by applicant]
WO WO2007061425A1 · 2007 [cited by applicant]
WO WO2008151127A1 · 2008 [cited by applicant]
WO WO2009073201A2 · 2009 [cited by applicant]
WO WO2010016937A2 · 2010 [cited by applicant]
WO WO2012027625A2 · 2012 [cited by applicant]
WO WO2013123258A1 · 2013 [cited by applicant]
WO WO2014171898A2 · 2014 [cited by applicant]
WO WO2015085268A1 · 2015 [cited by applicant]
WO WO2017007774A1 · 2017 [cited by applicant]
WO WO2017014762A1 · 2017 [cited by applicant]
WO WO2017117235A1 · 2017 [cited by applicant]
WO WO2018045109A1 · 2018 [cited by applicant]
WO WO2019018366A1 · 2019 [cited by applicant]
WO WO2019033062A2 · 2019 [cited by applicant]
WO WO2019241305A1 · 2019 [cited by applicant]
WO WO2020028194A1 · 2020 [cited by applicant]
WO WO2020076976A1 · 2020 [cited by applicant]
WO WO2020102594A1 · 2020 [cited by applicant]
WO WO2020102766A2 · 2020 [cited by applicant]
WO WO2020118255A1 · 2020 [cited by applicant]
WO WO2020223695A1 · 2020 [cited by applicant]
WO WO2020242901A1 · 2020 [cited by applicant]
WO WO2020243017A1 · 2020 [cited by applicant]
WO WO2021061841A1 · 2021 [cited by applicant]
WO WO2021146597A1 · 2021 [cited by applicant]
WO WO2021236792A1 · 2021 [cited by applicant]
WO WO2021252671A2 · 2021 [cited by applicant]
WO WO2022026891A1 · 2022 [cited by applicant]
WO WO2022094332A1 · 2022 [cited by applicant]
WO WO2022266470A1 · 2022 [cited by applicant]
WO WO2023004014A1 · 2023 [cited by applicant]
WO WO2022266462A3 · 2023 [cited by applicant]
WO WO2023107719A2 · 2023 [cited by applicant]
WO WO2023196924A2 · 2023 [cited by applicant]
WO WO2023205707A2 · 2023 [cited by applicant]
WO WO2024151556A1 · 2024 [cited by applicant]
WO WO2024173403A2 · 2024 [cited by applicant]
Gabbatiss: New form of DNA discovered inside living human cells. The Independent, accessed Apr. 28, 2018. (2018). [cited by applicant]
Joyce et al.: Fingers-closing and other rapid conformational changes in DNA polymerase I (Klenow fragment) and their role in nucleotide selectivity. Biochemistry 47(23):6103-6116 doi:10.1021/bi7021848 (2008). [cited by applicant]
Kramer et al.: Spanning binding sites on allosteric proteins with polymer-linked ligand dimers. Nature 395(6703):710-713 doi:10.1038/27227 (1998). [cited by applicant]
Krishnamurthy et al.: Dependence of Effective Molarity on Linker Length for an Intramolecular Protein-Ligand System. J. Am. Chem. Soc. 129(5):1312-1320 DOI:10.1021/JA066780E (2007). [cited by applicant]
Pack et al.: Tetravalent miniantibodies with high avidity assembling in [cited by applicant]
Terry: 10 Best Genome Sequencing Companies by Revenue. List of the best genome sequencing companies by there[sic] revenue. BioSpace Online, pp. 1-6 [Retrieved online Jun. 16, 2022] URL:https://www.biospace.com/article/t… [cited by applicant]
U.S. Appl. No. 17/144,945 Non-Final Office Action dated Jun. 6, 2022. [cited by applicant]
Anderson et al., Fluorescent Structural DNA Nanoballs Functionalized with Phosphate-Linked Nucleotide Triphosphates. Nano Letters 10(3): 788-792 (2010). [cited by applicant]
Batra et al., Magnesium-induced assembly of a complete DNA polymerase catalytic complex.. Structure 14(4):757-766 (2006). [cited by applicant]
Bentley, D. R. Whole-genome re-sequencing. Curr Opin Genet Dev. Dec. 2006;16(6):545-52. Epub Oct. 18, 2006. [cited by applicant]
Bentley et al. Accurate whole human genome sequencing using reversible terminator chemistry. Nature 456(7218):53-59 (2008). [cited by applicant]
Berki et al., Advanced Fluorescent Polymer Probes for the Site-Specific Labeling of Proteins in Live Cells Using the HaloTag Technology. ACS Omega 4: 12841-12847 (2019). [cited by applicant]
Related U.S. Appl. No. 63/059,723. [cited by applicant]
Dubber et al., Solid Phase Synthesis and Multivalent Glycoconjugates on a DNA Synthesizer. Bioconjugate Chem 14: 239-246 (2003). [cited by applicant]
Duret et al., Labeling of native proteins with fluorescent RAFT polymer probes: Application to the detection of a cell surface protein using flow cytometry. Polym. Chem. 9: 1857-1868 (2018). [cited by applicant]
Eschenmoser et al., Chemical etiology of nucleic acid structure. Science. 284(5423):2118-2124 (1999). [cited by applicant]
Favier et al., Synthesis on N-acryloxysuccinimide copolymers by RAFT polymerization, as reactive building blocks with full control of composition and molecular weights. Polymer 45: 7821-7830 (2004). [cited by applicant]
Ferraro et al., Biocatalytic selective modifications of conventional nucleosides, carbocyclic nucleosides, and C-nucleosides. Chem Rev. 100(12):4319-4348 (2000). [cited by applicant]
Gebeyehu et al., Novel biotinylated nucleotide—analogs for labeling and colorimetric detection of DNA. Nucleic Acids Research 15(11): 4513-4534 (1987). [cited by applicant]
Heather et al. The Sequence of Sequencers: The History of Sequencing DNA. Genomics 107:1-8 (2016). [cited by applicant]
Joeng et al., Structure-activity relationships of beta-D-(2S,5R)- and alpha-D-(2S,5S)-1,3-oxathiolanyl nucleosides as potential anti-HIV agents. J Med Chem. 36(18):2627-2638 (1993). [cited by applicant]
Ju et al. Four-Color DNA Sequencing by Synthesis Using Cleavable Fluorescent Nucleotide Reversible Terminators. PNAS USA 103:19635-19640 (2006). [cited by applicant]
Ju et al. Supporting Text—Four-Color DNA Sequencing by Synthesis Using Cleavable Fluorescent Nucleotide Reversible Terminators. PNAS USA 103:19635-19640 (2006). [cited by applicant]
Kim et al., 1,3-dioxolanylpurine nucleosides (2R,4R) and (2R,4S) with selective anti-HIV-1 activity in human lymphocytes. J Med Chem. 36(1):30-37 (1993). [cited by applicant]
Lorenz, et al. Polymerase chain reaction: basic protocol plus troubleshooting and optimization strategies. Journal of visualized experiments: JoVE 63 (2012). [cited by applicant]
Mardis. Next-Generation DNA Sequencing Methods. Annu Rev Genomics Hum Genet 9:387-402 (2008). [cited by applicant]
Martinez et al., Acyclic nucleoside triphosphate analogs as terminators in biocatalytic DNA replication. Bioorganic & Medicinal Chemistry Letters 7(23): 3013-3016 (1997). [cited by applicant]
Martinez et al., An allylic/acyclic adenosine nucleoside triphosphate for termination of DNA synthesis by DNA template-dependent polymerases. Nucleic Acids Res 27(5):1271-1274 (1999). [cited by applicant]
PCT/US2020/034409 International Search Report and Written Opinion dated Aug. 3, 2020. [cited by applicant]
PCT/US2020/052305 International Search Report and Written Opinion dated Feb. 1, 2021. [cited by applicant]
Sah et al., Complete genome sequence of a 2019 novel coronavirus (SARS-COV-2) strain isolated in Nepal. Microbiol Resour Announc. 9(11):e00169-20 (2020). [cited by applicant]
Singer et al., UV Spectral Characteristics and Acidic Dissociation Constants of 280 Alkyl Bases, Nucleosides, and Nucleotides. Practical Handbook of Bioschemistry and Molecular Biology. pp. 255-261 (2010). [cited by applicant]
Technology Spotlight: Illumina Sequencing (2010). [cited by applicant]
U.S. Appl. No. 17/144,945 Final Office Action dated Oct. 1, 2021. [cited by applicant]
U.S. Appl. No. 17/144,945 Non-Final Office Action dated Jun. 4, 2021. [cited by applicant]
U.S. Appl. No. 17/356,929 Final Office Action dated Nov. 22, 2021. [cited by applicant]
U.S. Appl. No. 17/356,929 Non-Final Office Action dated Jul. 27, 2021. [cited by applicant]
U.S. Appl. No. 16/579,794 Non-Final Office Action dated Jan. 30, 2020. [cited by applicant]
Zhang, et al. Reconstruction of DNA sequencing by hybridization. Bioinformatics. Jan. 2003;19(1):14-21. [cited by applicant]
Chen, et al., Expansion Microscopy. Science, Jan. 30, 2015; vol. 347, Issue 6221: 543-549. [cited by applicant]
Goransson et al.: A single molecule array for digital targeted molecular analyses. Nucleic Acids Res. 37(1):e7:1-9 doi:10.1093/nar/gkn921 (2009). [cited by applicant]
Cited By (4)
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