IP Library › Granted Patent US 12,245,594
Granted Patent B2
US 12,245,594 · App. 17/309,505 · Granted Mar 11, 2025

Methods for obtaining and using plants and plant parts with increased nutrient, oil, and/or protein content

Inventors: Patrick Vogan (St. Louis, MO); Janne Kerovuo (Los Angeles, CA); Charles Michael McFatrich (Washington, MO); Natalie Breakfield (St. Louis, MO)
Assignee: NewLeaf Symbiotics, Inc.
A01N63/20A23K10/30A23K20/147A23K20/158A23K20/174A23K20/22A23K20/24A23K20/26A23K20/30A23K50/10A23K50/30A23K50/75A23K50/80A23L33/105A23L33/115A23L33/15A23L33/16A23L33/185C12Q1/689
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Quick Facts
Patent No.
US 12,245,594
App. No.
17/309,505
Granted
Mar 11, 2025
Kind
B2
Abstract

Methods for identifying Methylobacterium strains which can be used to increase mineral nutrient, vitamins, oil and/or protein content in plants are provided. Also provided are related methods of providing seed lots, food ingredients, feed ingredients, food, or feed with increased mineral nutrient, vitamins, oil and/or protein content. Methods of providing increased mineral nutrient and/or vitamin content, and/or increased oil and/or protein yield from plant seed lots are also provided.

Claims (7)

1. A method of providing a seed lot with increased mineral nutrient, crude fat, oil, and/or protein content comprising harvesting a seed lot from mature plants grown from seeds and/or plants treated with an effective amount of a Methylobacterium strain, wherein the Methylobacterium strain is ISO20 (NRRL B-67743), ISO19 (NRRL B-67742), ISO18 (NRRL B-67741), ISO26 (NRRL B-67892), or variant thereof, wherein at least 95% of the seeds in the harvested seed lot are obtained from the mature plants, wherein the harvested seed lot is packaged, contained or otherwise segregated from seed obtained from untreated plants, and wherein at least 95% of the seed in the seed lot comprise the packaged, contained or otherwise segregated seed lot.

2. The method of claim 1 , wherein said variant of ISO20 (NRRL B-67743) comprises a sequence selected from the group consisting of SEQ ID NO: 25, 26, and 27; wherein said variant of ISO18 (NRRL B-67741) comprises a sequence selected from the group consisting of SEQ ID NO: 37, 38, and 39; wherein said variant of ISO19 (NRRL B-67742) comprises a sequence selected from the group consisting of SEQ ID NO: 49, 50, and 51; or wherein said variant of ISO26 (NRRL B-67892) comprises a sequence selected from the group consisting of SEQ ID NO: 74, 75, and 76.

3. The method of claim 1 , wherein said plants are soybean, Brassica sp., corn, sunflower, cotton, flax, alfalfa, rice, rye, wheat, barley, oat, sorghum, millet, safflower, or peanut plants.

4. The method of claim 1 , wherein the seeds and/or plants treated with an effective amount of a Methylobacterium strain are obtained by a soil treatment or application where the seed and/or plant is contacted and/or colonized by the Methylobacterium strain.

5. The method of claim 1 , wherein the seeds and/or plants treated with an effective amount of a Methylobacterium strain are obtained by spraying, coating, partially coating, immersing, and/or imbibing the plant or seeds with a composition comprising the Methylobacterium strain.

6. The method of claim 1 , wherein the seeds and/or plants treated with an effective amount of a Methylobacterium strain are obtained by spraying, immersing, and/or imbibing soil, a seed, a leaf, a fruit, a stem, a root, a tuber, or a shoot with a liquid, semi-liquid, emulsion, or slurry of a composition comprising the Methylobacterium strain.

7. The method of claim 1 , further comprising determining the mineral nutrient, crude fat, oil, and/or protein content of the seed lot.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Sep 17, 2021
From: VOGAN, PATRICK; KEROVUO, JANNE; MCFATRICH, CHARLES MICHAEL; BREAKFIELD, NATALIE
To: NEWLEAF SYMBIOTICS, INC.
Reel/Frame 057520/0107 →
Continuity (6)
Provisional Application 62900766 · Sep 16, 2019
Provisional Application 62878164 · Jul 24, 2019
Provisional Application 62846547 · May 10, 2019
Provisional Application 62802038 · Feb 6, 2019
Provisional Application 62774640 · Dec 3, 2018
Related Publication 20220015370A1 · Jan 20, 2022
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