Microbial fermentation methods and compositions
The present invention provides methods for the cultivation of the Methylobacterium genus of bacteria. In particular the method provides methods for the efficient and inexpensive cultivation of these bacteria. Additionally, the invention provides methods for the utilization of these bacterial cultures to improve plant agriculture.
1. A method for treating a plant or a plant part with Methylobacterium comprising the step of applying to said plant or plant part a composition comprising a fermentation product comprising a solid substance wherein a mono-culture or co-culture of Methylobacterium is adhered thereto by having been grown on said solid substance, wherein said solid substance comprises a plurality of particles of about 1 micron to about 1000 microns in average length or average diameter and is not a photosynthetic microorganism, and wherein said fermentation product is essentially free of contaminating microorganisms other than the Methylobacterium , and wherein said composition (i) has a Methylobacterium titer of at least 5×10 8 colony-forming units per ml of said composition to about 6×10 10 colony-forming units per ml of said composition for a fermentation broth, or (ii) has a Methylobacterium titer of at least 5×10 8 colony-forming units per gram of said composition to about 5×10 13 colony-forming units per gram of said composition for a dry composition.
2. The method of claim 1 , wherein said composition further comprises at least one of an agriculturally acceptable adjuvant or an agriculturally acceptable excipient.
3. The method of claim 1 , wherein said composition is an essentially dry product, an emulsion, or a suspension.
4. The method of claim 1 , wherein said plant part is a seed and said composition has a Methylobacterium titer of at least about 5×10 8 colony-forming units per gram of said composition to about 5×10 13 colony-forming units per gram of said composition.
5. The method of claim 1 , wherein said plant part is a seed, stem, root, flower, cotyledon, a coleoptile, fruit, or a leaf.
6. The method of anyone of claim 1 , wherein said plant is a corn, Brassica sp., alfalfa, rice, rye, sorghum, pearl millet, proso millet, foxtail millet, finger millet, sunflower, safflower, soybean, tobacco, potato, peanuts, cotton, sweet potato, cassava, coffee, coconut, pineapple, citrus trees, cocoa, tea, banana, avocado, fig, guava, mango, olive, papaya , cashew, macadamia, almond, sugar beet, sugarcane, oat, barley, tomato, lettuce, green bean, lima bean, pea, cucurbit, ornamental, or conifer plant.
7. The method of claim 1 , wherein said Methylobacterium can colonize a plant or plant part.
8. The method of claim 1 , wherein said fermentation product is harvested and dried.
9. The method of claim 8 , wherein said fermentation product is dried by lyophilization or spray drying.
10. The method of claim 1 , wherein said plant part is a seed and said composition comprises a fermentation broth product that has been partially or completely resuspended in a liquid.
11. The method of claim 10 , wherein said seed is treated by slurrying the seed with said composition.
12. The method of claim 11 , wherein said method further comprises drying the resulting product.
13. The method of claim 1 , wherein said plant is a corn plant, said part is a seed, and said composition is applied in an amount sufficient to provide for an increase in corn nodal root growth in a corn plant grown from the treated seed.
14. The method of claim 1 , wherein said plant is a corn plant, said part is a coleoptile, and/or a leaf, and said composition is applied in an amount sufficient to provide for an increase in corn nodal root growth in a corn plant comprising the treated coleoptile and/or a leaf.
15. The method of claim 11 , wherein said composition comprises an agriculturally acceptable adjuvant that promotes sticking to the seed.
16. The method of claim 11 , wherein said composition comprises an agriculturally acceptable adjuvant that promotes adhesion to and spreading of the composition on plant parts.
17. The method of claim 2 , wherein said composition comprises an agriculturally acceptable adjuvant selected from a wetter/spreader, a sticker, a penetrant, an extender, a humectant or combinations thereof.
18. The method of claim 1 , wherein said composition further comprises a pesticide.
19. The method of claim 18 , wherein said pesticide is at least one of an insecticide, a fungicide, a nematocide, and a bacteriocide, wherein said pesticide does not substantially inhibit growth of said Methylobacterium.