Methods and compositions for improving soybean yield
The present invention provides yield or early vigor enhancing compositions comprising Methylobacterium sp., methods for improving soybean yield, and methods of making the compositions. Also provided are isolated soybean yield or early vigor enhancing Methylobacterium sp.
1. A method for improving soybean plant yield wherein said method comprises:
1) coating or partially coating a soybean plant seed with:
i) a composition comprising an essentially dry fermentation product having about 5% or less water content, wherein said fermentation product comprises a Methylobacterium of NLS0064, deposited as NRRL B-50938; and
ii) an agriculturally acceptable excipient selected from the group consisting of woodflours, clays, activated carbon, diatomaceous earth, fine-grain inorganic solids and calcium carbonate; and/or
an agriculturally acceptable adjuvant selected from the group consisting of polyvinyl acetates, polyvinyl acetate copolymers, hydrolyzed polyvinyl acetates, polyvinylpyrrolidone-vinyl acetate copolymer, polyvinyl alcohols, polyvinyl alcohol copolymers, polyvinyl methyl ether, polyvinyl methyl ether-maleic anhydride copolymer, waxes, latex polymers, celluloses hydroxy methylcelluloses, hydroxypropylcellulose, hydroxymethylpropylcelluloses, polyvinyl pyrrolidones, alginates, dextrins, malto-dextrins, polysaccharides, fats, oils, proteins, karaya gum, jaguar gum, tragacanth gum, polysaccharide gums, mucilage, gum arabics, shellacs, vinylidene chloride polymers and copolymers, soybean-based protein polymers and copolymers, lignosulfonates, acrylic copolymers, starches, polyvinylacrylates, zeins, gelatin, carboxymethylcellulose, chitosan, polyethylene oxide, acrylamide polymers and copolymers, polyhydroxyethyl acrylate, methylacrylamide monomers, alginate, ethylcellulose, polychloroprene and syrups or mixtures thereof;
2) growing a soybean plant from said soybean plant seed; and
3) harvesting at least one soybean plant part from said soybean plant, wherein the Methylobacterium provides for increased yield of said soybean plant part in comparison to yield of said soybean plant part from a soybean plant grown from a soybean seed not treated with the Methylobacterium of NLS0064.
2. The method of claim 1 , wherein said clays and inorganic solids are selected from the group consisting of calcium bentonite, kaolin, china clay, talc, perlite, mica, vermiculite, silicas, quartz powder, montmorillonite and mixtures thereof.
3. The method of claim 1 , wherein said harvested plant part is soybean seed.
4. The method of claim 3 , wherein said soybean seed is harvested from a mature soybean plant.
5. The method of claim 1 , wherein said method further comprises obtaining a processed food or feed composition from said harvested plant part.
6. The method of claim 5 , wherein said processed food or feed composition is a meal or a paste.
7. The method of claim 1 , wherein the composition comprises the Methylobacterium at a titer of about 5×10 8 CFU/gm to about 6×10 10 CFU/gm.
8. A method of claim 1 , wherein said composition further comprises a Methylobacterium selected from the group consisting of: NLS0017, deposited as NRRL B-50931; and NLS0020, deposited as NRRL B-50930.
9. A composition comprising:
i) a population of soybean seeds for planting;
ii) an agriculturally acceptable excipient selected from the group consisting of woodflours, clays, activated carbon, diatomaceous earth, fine-grain inorganic solids and calcium carbonate; and/or
an agriculturally acceptable adjuvant selected from the group consisting of polyvinyl acetates, polyvinyl acetate copolymers, hydrolyzed polyvinyl acetates, polyvinylpyrrolidone-vinyl acetate copolymer, polyvinyl alcohols, polyvinyl alcohol copolymers, polyvinyl methyl ether, polyvinyl methyl ether-maleic anhydride copolymer, waxes, latex polymers, celluloses hydroxy methylcelluloses, hydroxypropylcellulose, hydroxymethylpropylcelluloses, polyvinyl pyrrolidones, alginates, dextrins, malto-dextrins, polysaccharides, fats, oils, proteins, karaya gum, jaguar gum, tragacanth gum, polysaccharide gums, mucilage, gum arabics, shellacs, vinylidene chloride polymers and copolymers, soybean-based protein polymers and copolymers, lignosulfonates, acrylic copolymers, starches, polyvinylacrylates, zeins, gelatin, carboxymethylcellulose, chitosan, polyethylene oxide, acrylamide polymers and copolymers, polyhydroxyethyl acrylate, methylacrylamide monomers, alginate, ethylcellulose, polychloroprene and syrups or mixtures thereof; and
iii) an essentially dry fermentation product having about 5% or less water content, wherein said fermentation product comprises a Methylobacterium of NLS0064, deposited as NRRL B-50938; wherein said fermentation product is essentially free of contaminating microorganisms.
10. The composition of claim 9 , wherein said essentially dry fermentation product is obtained by lyophilization or spray drying.
11. The composition of claim 9 , wherein said clays and inorganic solids are selected from the group consisting of calcium bentonite, kaolin, china clay, talc, perlite, mica, vermiculite, silicas, quartz powder, montmorillonite and mixtures thereof.
12. The composition of claim 9 , wherein said composition comprises an agriculturally acceptable adjuvant selected from the group consisting of a wetter/spreader, sticker, penetrant, extender, and humectant.
13. The composition of claim 9 , wherein said Methylobacterium is at a titer of about 1×10 6 CFU/gm to about 1×10 14 CFU/gm.
14. The composition of claim 9 , wherein said composition further comprises a Methylobacterium selected from the group consisting of: NLS0017, deposited as NRRL B-50931; and NLS0020, deposited as NRRL B-50930.
15. The composition of claim 9 , wherein the composition further comprises an active selected from the group consisting of an insecticide, a nematicide, a fungicide, and a biopesticidal or beneficial microorganism.
16. The composition of claim 15 , wherein said biopesticidal or beneficial microorganism is selected from the group consisting of Bacillus subtilis, Bacillus thuringiensis, Bacillus pumilis, Pseudomonas syringae, Trichoderma harzianum, Trichoderma virens , and Streptomyces lydicus.