Compositions and methods for improving fruit production
The present invention provides compositions comprising Methylobacterium and depleted of substances that promote growth of resident microorganisms on a plant or seed, methods for improving fruit production, methods of making the compositions, and methods of treating a plant or seed with a composition comprising Methylobacterium . Also provided are methods and compositions comprising Methylobacterium that provide for improved fruit production.
1. A method for improving fruit production, said method comprising:
(a) applying a composition comprising Methylobacterium and an agriculturally acceptable adjuvant, excipient, or combination thereof to a fruit bearing plant, wherein said Methylobacterium is NLS0037 (NRRL B-50941), NLS0038 (NRRL B-50942), NLS0042 (NRRL B-50932), NLS0062 (NRRL B-50937), and wherein said fruit bearing plant is an apple, pear, grape, citrus, melon, pepper, berry, kiwi, mango, or banana plant, and,
(b) harvesting fruit from said plant, wherein said plant exhibits faster fruit set, increased fruit set, earlier fruit maturation, and/or more uniform fruit maturation compared to an untreated control plant, thereby obtaining improved fruit production.
2. The method of claim 1 , wherein said fruit bearing plant is a pepper plant.
3. The method of claim 1 , wherein said composition is depleted of substances that promote growth of resident microorganisms on said plant or seed.
4. The method of claim 1 , wherein the Methylobacterium is NLS0062 (NRRL B-50937).
5. The method of claim 4 , wherein the Methylobacterium is NLS0037 (NRRL B-50941) or NLS0038 (NRRL B-50942).
6. The method of claim 1 , wherein said composition coats or partially coats said plant or a part thereof.
7. A method for improving fruit production, said method comprising:
(a) applying a composition comprising Methylobacterium to a seed of a fruit bearing plant wherein said Methylobacterium sp. is NLS0037 (NRRL B-50941), NLS0038 (NRRL B-50942), NLS0042 (NRRL B-50932), NLS0062 (NRRL B-50937), and an agriculturally acceptable adjuvant, excipient, or combination thereof and wherein said fruit bearing plant is an apple, pear, grape, citrus, melon, pepper, berry, kiwi, mango, or banana plant; and,
(b) harvesting fruit from a plant grown from said seed, wherein said plant grown from said seed exhibits faster fruit set, increased fruit set, earlier fruit maturation, and/or more uniform fruit maturation compared to an untreated control plant, thereby obtaining improved fruit production.
8. The method of claim 7 , wherein said composition comprises the Methylobacterium at a titer of about 1×10 6 colony-forming units per gram (CFU/gm) of solid to about 1×10 14 CFU/gm of solid for a composition comprising a solid substance with adherent Methylobacterium grown thereon or at a titer of about 1×10 6 CFU/mL to about 1×10 11 CFU/mL for a composition comprising an emulsion having the Methylobacterium grown therein.
9. The method of claim 7 , wherein said fruit bearing plant is a pepper plant.
10. The method of claim 7 , wherein the Methylobacterium is NLS0037 (NRRL B-50941) or NLS0038 (NRRL B-50942).
11. The method of claim 7 , wherein the Methylobacterium is NLS0042 (NRRL B-50932), NLS0062 (NRRL B-50937).
12. The method of claim 7 , wherein said composition coats or partially coats said seed.
13. A method of treating a fruit bearing plant or seed with a composition comprising Methylobacterium , said method comprising:
(a) obtaining a Methylobacterium preparation comprising a solid substance with the Methylobacterium grown thereon with a Methylobacterium titer of 1×10 6 colony-forming units per gram (CFU/gm) of solid to about 1×10 14 CFU/gm of solid or an emulsion with Methylobacterium grown therein at a titer of about 1×10 6 CFU/mL to about 1×10 11 CFU/mL, wherein said Methylobacterium is NLS0037 (NRRL B-50941), NLS0038 (NRRL B-50942), NLS0042 (NRRL B-50932), NLS0062 (NRRL B-50937); and
(b) applying said composition to a plant, a part thereof, or a seed, wherein said plant, part thereof, or seed is an apple, pear, grape, citrus, melon, pepper, berry, kiwi, mango, or banana plant, part thereof, or seed and wherein said plant or plant grown from said seed exhibits faster fruit set, increased fruit set, earlier fruit maturation, and/or more uniform fruit maturation compared to an untreated control plant, thereby obtaining improved fruit production.
14. The method of claim 1 , wherein the Methylobacterium in said composition are provided as a solid substance with adherent Methylobacterium grown thereon or as an emulsion having the Methylobacterium grown therein.
15. The method of claim 14 , wherein said composition comprises Methylobacterium at a titer of about 1×10 6 colony-forming units per gram (CFU/gm) of solid to about 1×10 14 CFU/gm of solid for the solid-containing composition or at a titer of about 1×10 6 CFU/mL to about 1×10 11 CFU/mL for the emulsion-containing composition.
16. The method of claim 14 , wherein the solid substance with the adherent Methylobacterium grown thereon is provided in a liquid or an emulsion.