Methods and compositions for controlling root lesion nematodes
The present disclosure provides compositions comprising Root-Lesion Nematode (RLN)-inhibitory Methylobacterium sp., as well as cell-free culture supernatants, fractions, and concentrates obtained from the Root-Lesion Nematode (RLN)-inhibitory Methylobacterium sp. Also provided are related methods for controlling RLN infections of plants, and methods of making the compositions.
1. A method for making a composition for treating a plant wherein said method comprises;
i) inoculating a liquid culture medium with Methylobacterium strain NLS0934 deposited as NRRL B-67341;
ii) growing the Methylobacterium strain in said medium to a titer of at least 5×10 8 CFU per ml; and
iii) harvesting the Methylobacterium from said medium.
2. The method of claim 1 , wherein said method comprises combining said Methylobacterium with an agriculturally acceptable excipient and/or with an agriculturally acceptable adjuvant.
3. The method of claim 1 , wherein said liquid culture medium is a biphasic media comprising a liquid phase and a solid substance.
4. The method of claim 1 , wherein said liquid culture medium is an emulsion.
5. The method of claim 2 , wherein said agriculturally excipient is a carrier.
6. The method of claim 5 , wherein said agriculturally acceptable excipient is a clay or inorganic solid selected from the group consisting of calcium bentonite, kaolin, china clay, talc, perlite, mica, vermiculite, silicas, quartz powder, montmorillonite and mixtures thereof.
7. The method of claim 2 , wherein said agriculturally acceptable adjuvant is a fat, oil or wax.
8. The method of claim 2 , wherein said agriculturally acceptable adjuvant is an acrylic or acrylamide polymer or copolymer.
9. The method of claim 2 , wherein said agriculturally acceptable adjuvant is a polysaccharide.
10. The method of claim 9 , wherein said polysaccharide is a dextrin, maltodextrin, starch, chitosan, alginate or gum.
11. The method of claim 2 , wherein said agriculturally acceptable adjuvant is a protein.
12. The method of claim 11 , wherein said protein is a soybean-based protein polymer, a zein or a gelatin.
13. The method of claim 2 , wherein said agriculturally acceptable adjuvant is a polyvinyl acetate, polyvinyl acetate copolymer, hydrolyzed polyvinyl acetate, polyvinylpyrrolidone-vinyl acetate copolymer, polyvinyl alcohol, polyvinyl alcohol copolymer, polyvinyl methyl ether, polyvinyl methyl ether-maleic anhydride copolymer, wax, latex polymer, cellulose, polyvinyl pyrrolidone, alginate, dextrin, maltodextrin, polysaccharide, fat, oil, protein, karaya gum, jaguar gum, tragacanth gum, polysaccharide gum, mucilage, gum arabic, shellac, vinylidene chloride polymer or copolymer, soybean-based protein polymer or copolymer, lignosulfonate, acrylic copolymer, starch, polyvinylacrylate, zein, gelatin, chitosan, polyethylene oxide, acrylamide polymer or copolymer, polyhydroxyethyl acrylate, methylacrylamide monomer, polychloroprene or a mixture thereof.
14. A composition for treating a plant or plant part to improve plant growth and/or yield, wherein said composition is essentially free of contaminating microorganisms, and comprises Methylobacterium NLS0934 deposited as NRRL B-67341; (ii) a derivative of NLS0934 deposited as NRRL B-67341 obtained by selection or by mutagenesis and selection of said deposited Methylobacterium ; or (iii) a genetically transformed strain obtained from said Methylobacterium NLS0934 deposited as NRRL B-67341; and wherein said composition further comprises an agriculturally acceptable adjuvant, excipient, or combination thereof.
15. The composition of claim 14 , wherein said composition is an essentially dry product having about 5% or less water content.
16. The essentially dry product of claim 15 , wherein said product is dried by lyophilization or spray drying.
17. The composition of claim 14 , wherein said composition further comprises a component that enhances product efficacy or enhances ease of product application to a plant or plant part.
18. The composition of claim 14 , wherein the agriculturally acceptable excipient comprises at least one component selected from the group consisting of calcium bentonite, kaolin, china clay, talc, perlite, mica, vermiculite, silicas, quartz powder, montmorillonite clay and mixtures thereof.
19. The composition of claim 14 , wherein said agriculturally acceptable adjuvant is selected from the group consisting of a wetter/spreader, sticker, penetrant, extender, and humectant.
20. The composition of claim 14 , wherein said agriculturally acceptable excipient is selected from the group consisting of woodflours, clays, activated carbon, diatomaceous earth, fine-grain inorganic solids, and calcium carbonate.
21. The composition of claim 14 , wherein said composition is adapted for use in treating a plant seed.
22. The composition of claim 14 , wherein said Methylobacterium is present at a titer of about 1×10 6 CFU/gm to about 1×10 14 CFU/gm.
23. The composition of claim 14 , wherein said agriculturally acceptable adjuvant comprises a maltodextrin.