Granted Patent B2

US 12,256,685 · App. 18/179,970 · Granted Mar 25, 2025

Inventors: Huachun Wang Larue (Chesterfield, MO); Li Lin (St. Louis, MO)

Assignee: Monsanto Technology LLC

A01H1/02C12Q1/6876C12Q2600/158

Abstract

Claims (19)

1. A method of producing a pollen suspension injection solution comprising a desired pollen concentration for cross-pollination comprising the steps of:

a) collecting pollen-shedding flowers from a male parent;

b) homogenizing said flowers to release pollen;

c) purifying pollen from said homogenized flowers by removing floral debris;

d) quantifying said purified pollen;

e) suspending said purified pollen in an injection solution to produce the pollen suspension injection solution comprising a desired pollen concentration, wherein the pollen suspension injection solution comprises 10% to 20% sucrose by weight; 0.04% to 0.08% xanthan gum by weight; and 1.5 units/L to about 1500 units/L pectin methylesterase (PME),

f) filling a syringe with the pollen suspension injection solution, and

g) injecting the pollen suspension injection solution into unopened flower buds.

2. The method of claim 1 , wherein the pollen is from a soybean plant.

3. The method of claim 1 , wherein the injection solution further comprises at least a first component selected from the group consisting of a thickening agent, a surfactant, a plant growth regulator, a mineral ion, a carrier protein, and a nucleic acid molecule.

4. The method of claim 3 , wherein the first component is a surfactant,

and wherein said surfactant comprises polyoxyethylene (20) sorbitan monolaurate.

5. The method of claim 4 , wherein the polyoxyethylene (20) sorbitan monolaurate is at a concentration of 0.001% to 0.01% by weight.

6. The method of claim 3 , wherein the first component is a mineral ion, and wherein said mineral ion is selected from the group consisting of MgSO 4 , ZnSO 4 , and boric acid.

7. The method of claim 6 , wherein said solution comprises 0.01% to 0.05% MgSO 4 by weight.

8. The method of claim 6 , wherein said solution comprises 0.01% to 0.05% ZnSO 4 by weight.

9. The method of claim 6 , wherein said solution comprises 0.005% to 0.02% boric acid by weight.

10. The method of claim 3 , wherein the first component is a carrier protein, and wherein said carrier protein comprises bovine serum albumin (BSA).

11. The method of claim 10 , wherein said solution comprises 0.01% to 0.1% bovine serum albumin (BSA) by weight.

Continuity (2)

References Cited (111)