Method for producing culture vessel, and culture vessel
In the production of a culture vessel in which a low cell-adhesive polymer as a coating agent is applied to a culture surface having a plurality of concave parts formed thereon, a use amount of the coating agent can be reduced. The low cell-adhesive polymer as the coating agent is applied to a surface of a culture base material in advance, and subsequently the culture base material is subjected to processing to form the culture vessel having the surface of the culture base material coated with the coating agent as the culture surface.
1. A method for producing a culture vessel, comprising:
applying a low cell-adhesion polymer as a coating agent to a surface of a culture base material in advance; and
subsequently processing the culture base material to form a culture vessel having the surface of the culture base material coated with the coating agent as a culture surface, wherein,
in the processing, a plurality of concave parts are formed on the surface of the culture base material by concave-convex processing, and the processed surface is used as the culture surface of the culture vessel.
2. The method for producing the culture vessel according to claim 1 , wherein
the culture base material is in a film or a sheet shape, and the culture vessel is in a bag shape.
3. The method for producing the culture vessel according to claim 1 , wherein
in the processing, the culture base material is formed in the bag shape, and subsequently the concave-convex processing is performed by at least any of blow molding, vacuum molding, and pressure molding.
4. The method for producing the culture vessel according to claim 1 , wherein
in the processing, the concave-convex processing is performed by heat transfer, and subsequently the culture base material is formed in the bag shape.
5. The method for producing the culture vessel according to claim 4 , comprising
applying a release agent to a mold used in the heat transfer; and
subsequently performing the concave-convex processing.
6. The method for producing the culture vessel according to claim 1 , wherein
a diameter of a circle or an inscribed circle of opening portions of the concave parts is from 1 mm or more to 10 mm or less.
7. The method for producing the culture vessel according to claim 1 , comprising
applying the coating agent only to the culture surface of the culture vessel.
8. The method for producing the culture vessel according to claim 1 , comprising
disposing a frame material on the culture base material; and
subsequently applying the coating agent to the culture base material to provide a heat-sealable region in the culture base material where the coating material is not applied.
9. The method for producing the culture vessel according to claim 1 , comprising
performing a hydrophilization treatment on the surface of the culture base material in advance; and
subsequently applying the coating material to the treated surface.
10. The method for producing the culture vessel according to claim 1 , wherein
a surface density of the low cell-adhesion polymer applied to the surface is from 100 mg/cm 2 to 1 mg/cm 2 .
11. The method for producing the culture vessel according to claim 1 , wherein the low cell-adhesion polymer applied to the surface of the culture base material is selected from a phospholipid polymer, a polyvinyl alcohol derivative, a phospholipid-polymer complex, polyhydroxyethylmethacrylate, a polyvinyl alcohol, agarose, chitosan, polyethylene glycol, and albumin.