Granted Patent
B2
US 12,264,344 · App. 15/502,955 · Granted Apr 1, 2025
Protein/oligonucleotide core-shell nanoparticle therapeutics
Assignee:
Northwestern University
C12N9/2471A61K38/44A61K38/47A61K47/549A61K47/6921C07K19/00C12N9/0065C12N9/96C12P3/00C12P19/02C12P19/14C12Y111/01006C12Y302/01023B82Y5/00Y02P20/582
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Abstract
The present disclosure is directed to core-shell nanoparticles, compositions comprising core-shell nanoparticles, and methods of their use.
Claims (49)
1. A method of inhibiting expression of a gene product encoded by a target polynucleotide comprising:
contacting a target polynucleotide with a nanoparticle comprising a core, a plurality of polynucleotides comprising a shell, and a linker,
wherein the linker connects the core to at least one polynucleotide of the plurality of polynucleotides comprising the shell and comprises:
Formula (I), (II), or both:
L and L 2 are each independently selected from C1-10 alkylene, —C(O)—C1-10 alkylene-Y— and —C(O)—C1-10 alkylene-Y—C1-10 alkylene-(OCH 2 CH 2 )m-Y—;
each Y is independently selected from the group consisting of a bond, C(O), O, NH, C(O)NH, and NHC(O);
m is 0, 1, 2, 3, 4, or 5; and
PN is the at least one polynucleotide of the plurality of polynucleotides comprising the shell,
wherein the core is a single protein,
wherein the single protein exhibits catalytic activity, the single protein does not exhibit transport activity and the single protein is not albumin,
wherein the plurality of polynucleotides comprising the shell are attached to the surface of the single protein via covalent bonds at a surface density of at least 10 pmol/cm 2 ,
wherein at least one polynucleotide of the plurality of polynucleotides comprising the shell is attached to the single protein surface via a surface amino group from a lysine residue of the single protein,
wherein at least one polynucleotide of the plurality of polynucleotides is sufficiently complementary to the target polynucleotide to hybridize under conditions sufficient to inhibit expression of the gene product by at least 10%, and
wherein at least one polynucleotide of the plurality of polynucleotides is 5 to 40 nucleotides in length.
2. The method of claim 1 , wherein the single protein is not a fragment.
3. The method of claim 1 , wherein at least one polynucleotide of the plurality of polynucleotides is 5 to 30 nucleotides in length.
4. The method of claim 1 , wherein the single protein is beta galactosidase or catalase.
5. The method of claim 1 , wherein the shell attached to the surface of the single protein via covalent bonds are at a surface density of at least 15 pmol/cm 2 .
6. The method of claim 1 , wherein the shell attached to the surface of the single protein via covalent bonds are at a surface density of at least 20 pmol/cm 2 .
7. The method of claim 1 , wherein the shell attached to the surface of the single protein via covalent bonds are at a surface density of at least 25 pmol/cm 2 .
8. The method of claim 1 , wherein the shell attached to the surface of the single protein via covalent bonds are at a surface density of at least 30 pmol/cm 2 .
9. The method of claim 1 , wherein the shell attached to the surface of the single protein via covalent bonds are at a surface density of at least 35 pmol/cm 2 .
10. The method of claim 1 , wherein the shell attached to the surface of the single protein via covalent bonds are at a surface density of at least 40 pmol/cm 2 .
11. The method of claim 1 , wherein expression of the gene product is inhibited by at least 10% compared to gene product expression in the absence of the nanoparticle.
12. A method of inhibiting expression of a gene product encoded by a target polynucleotide comprising:
contacting a target polynucleotide with a nanoparticle comprising a core a plurality of polynucleotides comprising a shell, and a linker,
wherein the linker connects the core to at least one polynucleotide of the plurality of polynucleotides comprising the shell and comprises:
Formula (I), (II), or both:
L and L 2 are each independently selected from C1-10 alkylene, —C(O)—C1-10 alkylene-Y—, and —C(O)—C1-10 alkylene-Y—C1-10 alkylene-(OCH 2 CH 2 )m-Y—;
each Y is independently selected from the group consisting of a bond, C(O), O, NH, C(O)NH, and NHC(O);
m is 0, 1, 2, 3, 4, or 5; and
PN is the at least one polynucleotide of the plurality of polynucleotides comprising the shell,
wherein the core is a single protein,
wherein the single protein:
(a) exhibits catalytic activity and exhibits therapeutic activity, but the single protein is not albumin, or
(b) exhibits catalytic activity and exhibits therapeutic activity, but the single protein is not a fragment,
wherein the plurality of polynucleotides comprising the shell are attached to the surface of the single protein via covalent bonds at a surface density of at least 10 pmol/cm 2 ,
wherein at least one polynucleotide of the plurality of polynucleotides comprising the shell is attached to the single protein surface via a surface amino group from a lysine residue of the single protein,
wherein at least one polynucleotide of the plurality of polynucleotides is sufficiently complementary to the target polynucleotide to hybridize under conditions sufficient to inhibit expression of the gene product by at least 10%, and
wherein at least one polynucleotide of the plurality of polynucleotides is 5 to 40 nucleotides in length.
13. The method of claim 12 , wherein at least one polynucleotide of the plurality of polynucleotides is 5 to 30 nucleotides in length.
14. The method of claim 12 , wherein each polynucleotide of the plurality of polynucleotides is 5 to 30 nucleotides in length.
15. The method of claim 12 , wherein the shell attached to the surface of the single protein via covalent bonds are at a surface density of at least 15 pmol/cm 2 .
16. The method of claim 12 , wherein the shell attached to the surface of the single protein via covalent bonds are at a surface density of at least 20 pmol/cm 2 .
17. The method of claim 12 , wherein the shell attached to the surface of the single protein via covalent bonds are at a surface density of at least 25 pmol/cm 2 .
18. The method of claim 12 , wherein the shell attached to the surface of the single protein via covalent bonds are at a surface density of at least 30 pmol/cm 2 .
19. The method of claim 12 , wherein the shell attached to the surface of the single protein via covalent bonds are at a surface density of at least 35 pmol/cm 2 .
20. The method of claim 12 , wherein the shell attached to the surface of the single protein via covalent bonds are at a surface density of at least 40 pmol/cm 2 .
21. The method of claim 12 , wherein expression of the gene product is inhibited by at least 10% compared to gene product expression in the absence of the nanoparticle.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded May 26, 2017
From: MIRKIN, CHAD A.; BRODIN, JEFFREY D.
To: NORTHWESTERN UNIVERSITY
Reel/Frame 042517/0415 →
Continuity (4)
Provisional Application
62137183
· Mar 23, 2015
Provisional Application
62039608
· Aug 20, 2014
Provisional Application
62039340
· Aug 19, 2014
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