IP Library › Granted Patent US 12,297,474
Granted Patent B2
US 12,297,474 · App. 18/286,791 · Granted May 13, 2025

Process to produce mono-rhamnolipids

Inventors: Natalia Eliza Iyke Domeradzka (Liverpool, GB); Dietmar Andreas Lang (Liverpool, GB); Neil James Parry (Tarporley, GB); Mark Lawrence Thompson (Ellesmere Port, GB)
Assignee: Conopco, Inc.
C12P19/44C12N9/2402C12N11/00C12Y302/0104
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Quick Facts
Patent No.
US 12,297,474
App. No.
18/286,791
Granted
May 13, 2025
Kind
B2
Abstract

The invention concerns a process to convert di-rhamnolipid to mono-rhamnolipid comprising the following process steps: —(a) contact of a starting di-rhamnolipid material with an α-L-rhamnosidase enzyme which is immobilised on a support; (b) separation of the produced mono-rhamnolipid from the reaction medium and/or side products; wherein the α-L-rhamnosidase enzyme does not have β-D-glucosidase activity.

Claims (17)

1. A process to convert di-rhamnolipid to mono-rhamnolipid comprising the following process steps:

(a) contacting a starting di-rhamnolipid material with an α-L-rhamnosidase enzyme which is immobilised on a support;

(b) separating a produced mono-rhamnolipid from a reaction medium and/or side products;

wherein the α-L-rhamnosidase enzyme does not have β-D-glucosidase activity and is from Aspergillus lentulus , and the resulting mono-rhamnolipid material has a carbon alkyl length of from C 8 to C 14 .

2. The process according to claim 1 , wherein the enzyme is immobilised on a support using a technique selected from adsorption, covalent bonding, entrapment, crosslinking, and any combination thereof.

3. The process according to claim 1 , wherein the starting di-rhamnolipid material has a carbon alkyl length of from C 8 to C 14 .

4. The process according to claim 3 , wherein the starting di-rhamnolipid material has a carbon alkyl length of from C 8 -C 12 .

5. The process according to claim 1 , wherein a rhamnose by-product is removed from the enzymatic reaction mixture as the reaction progresses.

6. The process according to claim 1 , wherein a temperature during the reaction is from 10 to 60° C.

7. The process according to claim 6 , wherein the temperature during the reaction is from 15 to 50° C.

8. The process according to claim 6 , wherein the temperature during the reaction is from 18 to 45° C.

9. The process according to claim 6 , wherein the temperature during the reaction is from 20 to 45° C.

10. The process according to claim 1 , wherein a pH during the reaction is from pH 5 to 10.

11. The process according to claim 10 , wherein the pH during the reaction is from pH 5 to 9.

12. The process according to claim 10 , wherein the pH during the reaction is from pH 5.5 to 8.5.

13. The process according to claim 10 , wherein the pH during the reaction is from pH 6 to 8.

14. The process according to claim 1 , wherein the resulting mono-rhamnolipid material has a carbon alkyl length of from C 8 -C 12 .

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 12, 2023
From: DOMERADZKA, NATALIA ELIZA IYKE; LANG, DIETMAR ANDREAS; PARRY, NEIL JAMES; THOMPSON, MARK LAWRENCE
To: CONOPCO, INC., D/B/A UNILEVER
Reel/Frame 065843/0232 →
Priority Claims (1)
EP 21171230 · Apr 29, 2021 · regional
Continuity (1)
Related Publication 20240084353A1 · Mar 14, 2024
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