Compositions and method of use for hair straightening and shaping
Peptides are provided herein, wherein the peptide bearing one or more amino acid sequence differences relative to SEQ ID NO: 1, with the amino acid sequence differences comprising at least one cysteine replacement or addition.
1. A method comprising:
applying to hair a composition comprising a plurality of a peptide comprising substitution of an at least one amino acid of SEQ ID NO:1 with a cysteine, wherein the peptide comprises at least 50% sequence identity to SEQ ID NO:1.
2. The method of claim 1 , wherein the peptide comprises the amino acid sequence of any one of SEQ ID NO:8, SEQ ID NOS:3-7, or SEQ ID NOS:9-109, or a variant thereof with at least 70% sequence identity thereto.
3. The method of claim 1 , wherein the cysteine substitution occurs at any one or more of a glutamine amino acid position, a valine amino acid position, an alanine amino acid position, and/or a serine amino acid position.
4. The method of claim 3 , wherein the peptide comprises a Q13C substitution relative to amino acid sequence of SEQ ID NO:1.
5. The method of claim 3 , wherein the peptide comprises an H6C substitution relative to amino acid sequence of SEQ ID NO:1.
6. The method of claim 3 , wherein the peptide comprises a Q13C or H6C substitution and at least one additional cysteine substitution at any other amino acid position relative to SEQ ID NO:1.
7. The method of claim 6 , wherein the at least one additional cysteine substitution is >1 and 11 amino acids relative to SEQ ID NO:1.
8. The method of claim 1 , wherein the peptide comprises substitution of at least 3 amino acids of SEQ ID NO:1 with cysteine.
9. The method of claim 6 , wherein the at least one additional cysteine substitution is >3 and <11 amino acids relative to SEQ ID NO:1.
10. The method of claim 2 , wherein the peptide consists of the amino acid sequence of any one of SEQ ID NO:8, SEQ ID NOS: 3-7, or SEQ ID NOS:9-109.
11. The method of claim 1 , wherein the peptide comprises 1 comprising the amino acid sequence of SEQ ID NO:8, or a variant thereof with at least 70% sequence identity thereto.
12. The method of claim 1 , wherein a plurality of the peptides contacted with hair, optionally damaged hair, increases hair strength greater than the same number of peptides of SEQ ID NO:1 contacted with hair under the same conditions.
13. The method of claim 1 , wherein the peptide binds keratin.
14. The method of claim 13 , wherein a keratin-bound peptide is more resistant to washing than a keratin bound peptide consisting of the amino acid sequence of SEQ ID NO:1 contacted with hair under the same conditions.
15. The method of claim 1 , wherein the composition is a liquid.
16. The method of claim 1 comprising about 0.01% to about 0.1% w/w of the peptide.
17. The method of claim 1 , wherein the composition is selected from the group consisting of a shampoo, a conditioner, an oil, or a mask.
18. The method of claim 1 , wherein the applying step occurs subsequent to application of a hair waving formulation, a hair straightening formulation, a hair coloring formulation, or a hair bleaching formulation to the hair.
19. The method of claim 1 , wherein the peptide forms an alpha helix and at least one cysteine substitution is located on the outside region of the alpha helix.
20. The method of claim 1 , wherein one cysteine substitution is located in the region of SEQ ID NO:1 consisting of SEQ ID NO:2.
21. The method of claim 20 , wherein the one cysteine substitution located in the region of SEQ ID NO:1 consisting of SEQ ID NO:2 is a V17C substitution relative to the amino acid sequence of SEQ ID NO:1.
22. The method of claim 1 , wherein during the applying step the composition is applied by hand, applicator bottle, applicator brush, dropper, or spray bottle.
23. The method of claim 1 , wherein during the applying step the hair is saturated with the composition.
24. The method of claim 1 , wherein following the applying step the hair is not washed for at least 24 hours.
25. The method of claim 1 , wherein the peptide consists of the amino acid sequence of SEQ ID NO:8.