Granted Patent B2

US 12,310,356 · App. 17/015,443 · Granted May 27, 2025

Inventors: Peter De Muylder (Londerzeel, BE); David Kravitz (Barrington Hills, IL); Christopher P Steinman (Sandy, UT); David Pettinato (Schaumburg, IL); Bernard Theunis (Lubbeek, BE)

Assignee: LIFELINE SCIENTIFIC, INC.

A01N1/143

Abstract

Claims (42)

1. An apparatus for at least one of perfusion and transport of an organ or tissue, the apparatus comprising:

an organ container for storing the organ or tissue, the organ container comprising:

a basin configured to hold the organ or tissue and a perfusate bath;

at least one internal lid configured to cover the basin;

tubing that (i) is connectable to a source of oxygen, (ii) includes a plurality of holes by which the oxygen may exit the tubing, and (iii) is located within the basin so as to be submerged within the perfusate bath present during the perfusion or transport of the organ or tissue; and

a connector within the basin;

an oxygen line configured to convey oxygen from the source of oxygen to the tubing, a first end of the oxygen line being within the basin of the organ container and a second end of the oxygen line being outside the basin;

a first external lid; and

a second external lid adjacent to but not covering the first external lid, the first and second external lids each forming at least a part of a topmost surface of the apparatus when closed, wherein:

the connector within the basin is configured to connect and disconnect the tubing within the basin with the oxygen line that extends outside of the basin;

the first external lid is configured to cover the basin when closed;

the second external lid does not cover the basin when closed but is instead configured to cover other components of the apparatus, including the second end of the oxygen line, when closed;

the oxygen line, from the first end to the second end, is covered by the first and second external lids when the first and second external lids are closed; and

the second end of the oxygen line is exposed when the first external lid is closed and the second external lid is open.

2. The apparatus of claim 1 , wherein the organ container further comprises at least one holder within the basin to secure the tubing below a surface of the perfusate bath.

3. The apparatus of claim 2 , wherein:

the at least one holder is a plurality of the holders; and

each of the plurality of holders:

(i) extends toward a bottom of the basin from an upper rim of the basin;

(ii) descends along an internal wall of the basin; and

(iii) includes a hole through which the tubing passes.

4. The apparatus of claim 2 , wherein the at least one holder secures the tubing in a loop that encircles the organ or tissue in use.

5. The apparatus of claim 1 , wherein the connector is connected to the tubing by another tubing within the basin and a T-fitting.

6. The apparatus of claim 1 , wherein the connector is a Luer Lock fitting.

7. The apparatus of claim 1 , wherein the holes are arranged in a plurality of groupings spaced apart along a length of the tubing.

8. The apparatus of claim 7 , wherein each of the groupings comprises a plurality of the holes spaced apart around a circumference of the tubing.

9. The apparatus of claim 8 , wherein:

each pair of the plurality of groupings are spaced apart by about 34.79 mm of the tubing; and

an average diameter of each of the plurality of holes is between 0.10 mm and 0.18 mm.

10. The apparatus of claim 1 , further comprising an anti-bacterial filter at the second end of the oxygen line.

11. The apparatus of claim 1 ,

wherein the at least one internal lid is located between the first external lid and the basin when the first external lid is closed.

12. A method of oxygenating a perfusate solution to be perfused through an organ or tissue, the method comprising:

introducing the perfusate solution into the apparatus of claim 1 so as to form in the basin of the organ container a perfusate bath within which the tubing inside the basin is submerged;

connecting the tubing to a source of oxygen; and

administering oxygen from the source, through the holes in the tubing, and into the perfusate bath so as to increase oxygen concentration of the perfusate solution constituting the bath.

13. The method of claim 12 , further comprising administering the oxygen from the oxygen source at a rate of about 10 liters per minute for at least 10 minutes.

14. The method of claim 12 , further comprising:

disconnecting the tubing from the source of oxygen by way of the connector within the basin so that the oxygen from the source is administered in a space within the basin above a surface of the perfusate bath;

reducing a flow rate of the administered oxygen;

introducing an organ or tissue into the basin of the organ container; and

perfusing the organ or tissue with the perfusate solution while the oxygen is being administered in the space within the basin and above the surface of the perfusate bath.

Assignments (1)

Continuity (2)

References Cited (100)