Organoid produced using carrier for cell culture, and method for evaluating drug toxicity using same
View Patent ↗The present invention relates to an organoid and, more specifically, to an organoid and a use thereof, the organoid being produced using a carrier for cell culture which comprises microcapsules containing gelatin, a natural polymer, an oil, and an oil thickener. When used as a carrier for cell culture in culturing cells, the microcapsules containing a natural oil, according to the present invention, have the effects of improving adhesion and survival of the cells and inducing maturation of the cultured cells. The organoid produced by culturing cells using the carrier for cell culture has been confirmed to have the function of the organ concerned and, when treated with a drug, react to the toxicity of the drug and thus may be variously employed in the development of new drugs, disease research, and the field of artificial organ development.
1. A method for organoid production, comprising co-culturing mammalian cells and a carrier for three-dimensional (3D) mammalian cell culture, the carrier comprising microcapsules each having a wall containing gelatin and a natural polymer, surrounding a core containing an oil, and dextrin palmitate as an oil thickener, to produce organoids, wherein
the natural polymer is one or more selected from the group consisting of Arabic gum, hyaluronic acid, guar gum, pectin, xanthan gum, locust bean gum, tamarind gum, tragacanth gum, gum ghatti, locust bean gum, Konjac gum, agar, Carragheenan, furcellaran, and gellan, and wherein
the oil is one or more selected from the group consisting of olive oil, camellia oil, castor oil, palm oil, Jojoba oil, almond oil, grapeseed oil, herbal oil, rose oil, coconut oil, moringa oil, rice bran oil, apricot kernel oil, sunflower oil, meadowfoam seed oil, Abyssinian oil, and squalane.
2. The method of claim 1 , wherein the microcapsules are prepared by mixing the gelatin and the natural polymer at a weight ratio of 1:0.1 to 1.
3. The method of claim 2 , wherein the natural polymer is a mixture of Arabic gum and hyaluronic acid.
4. The method of claim 3 , wherein the Arabic gum and hyaluronic acid are mixed at a weight ratio of 1:9 to 9:1.
5. The method of claim 1 , wherein the oil thickener is contained in an amount of 1 to 15 wt % with respect to the weight of the oil.
6. The method of claim 1 , wherein the mammalian cells are anchorage-dependent cells.
7. The method of claim 6 , wherein the anchorage-dependent cells are one or more selected from the group consisting of cardiomyocytes, vascular endothelial cells, lipocytes, epithelial cells, fibroblasts, osteoblasts, chondrocytes, hepatocytes, uterine cervical cells, cancer cells, and mesenchymal stem cells.