Granted Patent
B2
US 12,365,889 · App. 18/485,895 · Granted Jul 22, 2025
Tunable nanoscale cages from self-assembling DNA and protein building blocks
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Abstract
Described herein are polyhedral, three-dimensional tunable nanocages assembled with a multimeric protein covalently linked to a polynucleotide handle and a DNA origami base assembly including sequences complementary to the polynucleotide handles, wherein the polynucleotide handle and the complementary sequences hybridize to for double-stranded DNA helices.
Claims (11)
1. A polyhedral nanocage comprising:
a multimeric protein, wherein each monomer of the multimeric protein is covalently linked to a polynucleotide handle; and
a DNA assembly of at least 4 oligonucleotides comprising a polynucleotide arm complementary to the polynucleotide handle, wherein the polynucleotide handle and the polynucleotide arm are hybridized to form a double stranded DNA helix linking the multimeric protein to the DNA assembly;
wherein polynucleotide handle is at least 15 base pairs long;
wherein the polynucleotide handle is covalently linked to a solvent-exposed cysteine residue by a disulfide linkage; and
wherein the multimeric protein comprises a surface 4-azidophenylalanine residue.
2. The polyhedral nanocage of claim 1 , wherein the polynucleotide handle is covalently linked to the 4-azidophenylalanine residue.
3. The polyhedral nanocage of claim 1 , wherein each oligonucleotide of the DNA assembly is at least 15 base pairs long.
4. The polyhedral nanocage of claim 3 , wherein each oligonucleotide of the DNA assembly is at least 21 base pairs long.
5. The polyhedral nanocage of claim 1 , additionally comprising a payload.
6. The polyhedral nanocage of claim 1 , wherein the multimeric protein is scaffold for the attachment of a small molecule, a targeting peptide, an antibody, or a fusion protein.
Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST
Recorded Nov 10, 2023
From: STEPHANOPOULOS, NICHOLAS; XU, YANG
To: ARIZONA BOARD OF REGENTS ON BEHALF OF ARIZONA STATE UNIVERSITY
Reel/Frame 065520/0918 →
Continuity (3)
Provisional Application
62816729
· Mar 11, 2019
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