IP Library › Granted Patent US 12,371,354
Granted Patent B2
US 12,371,354 · App. 18/109,658 · Granted Jul 29, 2025

Method of facilitating growth of specific microorganisms

Inventors: Martin Gross (Boone, IA); Zhiyou Wen (Ames, IA); Xuefei Zhao (Ames, IA); Max Thomas Gangestad (Boone, IA)
Assignee: Gross-Wen Technologies, Inc.
C02F3/322A01D44/00A01G33/00C02F3/08C12N1/12C02F2101/105C02F2203/006Y02A40/80Y02W10/10Y02W10/37
View Patent ↗
Loading inventors, assignments & file history…
Monitor This Case
Get email alerts when status or documents change.
Order Certified Copies
Most orders are placed with the USPTO same day — all within 24 business hours.
Order via The Patent Place →
Pre-filled with this patent's details
Quick Facts
Patent No.
US 12,371,354
App. No.
18/109,658
Granted
Jul 29, 2025
Kind
B2
Abstract

A method of using algae to remove a contaminant or pollutant from a first fluid is provided. The method can include providing a growing apparatus having a first reservoir containing the first fluid and a second reservoir containing a second fluid, and growing the algae using the growing apparatus. The method can further include exposing the algae to the first fluid within the first reservoir where the algae uptakes the contaminant or pollutant from the first fluid, and exposing the algae via a belt to the second fluid in the second reservoir where the algae is stimulated to release the contaminant or pollutant. Exposing the algae to the first fluid within the first reservoir or the second fluid may change a growth rate of the algae.

Claims (36)

1. A method of using algae to remove a pollutant from a first fluid, the method comprising:

providing an apparatus having a first reservoir containing the first fluid and a second reservoir containing a second fluid;

associating the apparatus with algae;

exposing the algae to the first fluid within the first reservoir wherein the algae uptakes the pollutant from the first fluid; and

exposing the algae to the second fluid in the second reservoir wherein the algae is stimulated to release the pollutant,

wherein exposing the algae to the first fluid or to the second fluid changes a growth rate of the algae, and

wherein following release of the pollutant, the algae is then brought back to the first reservoir to uptake additional pollutants.

2. The method of claim 1 , wherein exposing the algae to the first fluid or exposing the algae to the second fluid facilitates the growth rate of the algae.

3. The method of claim 2 , wherein exposing the algae to the first fluid or exposing the algae to the second fluid facilitates the growth rate of a predetermined species of the algae.

4. The method of claim 1 , wherein the algae is exposed to the second fluid via a belt, wherein the belt moves in a continuous manner.

5. The method of claim 4 , wherein the belt comprises a surface and microorganisms growing on the surface.

6. The method of claim 5 , wherein the belt comprises bacteria, fungi, the algae, or a combination thereof.

7. The method of claim 6 , wherein exposing the algae to the first fluid or exposing the algae via the belt to the second fluid facilitates a growth rate of the bacteria, the fungi, the algae, or the combination thereof.

8. The method of claim 1 , wherein the pollutant is nitrogen, phosphorous, potassium, carbon, a toxic metal, a salt, a pharmaceutical, a pharmaceutical and personal care product, or a hormone.

9. The method of claim 1 , further comprising starving the algae of the pollutant by exposing the algae to the second fluid thereby causing the algae to consume increased amounts of the pollutant from the first fluid in the first reservoir.

10. The method of claim 1 , further comprising concentrating the pollutant within the second fluid in the second reservoir.

11. The method of claim 1 , wherein a first temperature of the first fluid is lower than a second temperature of the second fluid.

12. The method of claim 11 , further comprising controlling the second temperature of the second fluid.

13. The method of claim 1 , wherein the second fluid is illuminated using increased light intensity.

14. The method of claim 1 , wherein the algae is exposed to a sorbent material.

15. The method of claim 1 , further comprising controlling a temperature of air or light within the apparatus.

16. The method of claim 1 , further comprising harvesting the algae.

17. A method of using microorganisms to remove a pollutant from a first fluid, the method comprising:

providing an apparatus having a first reservoir containing the first fluid and a second reservoir containing a second fluid;

associating the apparatus with microorganisms;

exposing the microorganisms to the first fluid within the first reservoir wherein the microorganisms uptake the pollutant from the first fluid; and

exposing the microorganisms to the second fluid in the second reservoir wherein the microorganisms release the pollutant,

wherein exposing the microorganisms to the first fluid or to the second fluid changes a growth rate of the microorganisms, and

wherein the microorganisms are starved of the pollutant by exposing the microorganisms to the second fluid thereby causing the microorganisms to consume increased amounts of the pollutant from the first fluid in the first reservoir.

18. A method of using microorganisms to remove a contaminant from a first fluid, the method comprising:

providing an apparatus having a first reservoir containing the first fluid and a second reservoir containing a second fluid;

associating the apparatus with microorganisms;

exposing the microorganisms to the first fluid within the first reservoir wherein the microorganisms uptake the contaminant from the first fluid; and

exposing the microorganisms to the second fluid in the second reservoir wherein the microorganisms release the contaminant,

wherein exposing the microorganisms to the first fluid or to the second fluid changes a growth rate of the microorganisms, and

wherein the microorganisms are starved of the pollutant by exposing the microorganisms to the second fluid thereby causing the microorganisms to consume increased amounts of the pollutant from the first fluid in the first reservoir.

Assignments (2)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 15, 2023
From: GROSS, MARTIN; WEN, ZHIYOU; ZHAO, XUEFEI
To: GROSS-WEN TECHNOLOGIES, INC.
Reel/Frame 062709/0955 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Feb 15, 2023
From: GANGESTAD, MAX THOMAS
To: GROSS-WEN TECHNOLOGIES, INC.
Reel/Frame 062710/0098 →
Continuity (5)
Continuation 17405378 · Aug 18, 2021
Continuation 17124753 · Dec 17, 2020
Continuation 16534586 · Aug 7, 2019
Provisional Application 62715485 · Aug 7, 2018
Related Publication 20230183114A1 · Jun 15, 2023
References Cited (63)
US 3565797A · Gresham · 1971 [cited by applicant]
US 3598726A · Welch · 1971 [cited by applicant]
US 3951805A · Dodd · 1976 [cited by examiner]
US 4324068A · Anthony · 1982 [cited by applicant]
US 4554390A · Curtain et al. · 1985 [cited by applicant]
US 5647983A · Limcaco · 1997 [cited by applicant]
US 5778823A · Adey · 1998 [cited by examiner]
US 6158386A · Limcaco · 2000 [cited by applicant]
US 6667171B2 · Bayless et al. · 2003 [cited by applicant]
US 6794184B1 · Mohr et al. · 2004 [cited by applicant]
US 8372631B2 · Shepherd · 2013 [cited by applicant]
US 8377687B2 · Shepherd · 2013 [cited by applicant]
US 8920810B2 · Adey et al. · 2014 [cited by applicant]
US 9932549B2 · Gross et al. · 2018 [cited by applicant]
US 10125341B2 · Wen et al. · 2018 [cited by applicant]
US 10570359B2 · Gross et al. · 2020 [cited by applicant]
US 20080135474A1 · Limcaco · 2008 [cited by applicant]
US 20090203116A1 · Bazaire · 2009 [cited by examiner]
US 20090230040A1 · Limcaco · 2009 [cited by applicant]
US 20100144017A1 · Shepherd · 2010 [cited by applicant]
US 20100224574A1 · Youngs et al. · 2010 [cited by applicant]
US 20100267122A1 · Chinnasamy et al. · 2010 [cited by applicant]
US 20110023565A1 · Yanik · 2011 [cited by examiner]
US 20110070632A1 · Katoch et al. · 2011 [cited by applicant]
US 20110217764A1 · Christenson et al. · 2011 [cited by applicant]
US 20110258915A1 · Subhadra · 2011 [cited by applicant]
US 20110263886A1 · Kale · 2011 [cited by applicant]
US 20110283608A1 · Patel et al. · 2011 [cited by applicant]
US 20110312062A1 · Nordvik et al. · 2011 [cited by applicant]
US 20120018373A1 · Jones et al. · 2012 [cited by applicant]
US 20120077253A1 · Burkhead · 2012 [cited by examiner]
US 20120152832A1 · Johnson et al. · 2012 [cited by applicant]
US 20120252105A1 · Ahrens et al. · 2012 [cited by applicant]
US 20130164836A1 · Licamele · 2013 [cited by examiner]
US 20130269244A1 · Jovine · 2013 [cited by examiner]
US 20140127776A1 · Picard · 2014 [cited by examiner]
US 20140273171A1 · Gross et al. · 2014 [cited by applicant]
US 20140273174A1 · Gross et al. · 2014 [cited by applicant]
US 20150136037A1 · Boonekamp · 2015 [cited by examiner]
US 20160039693A1 · Kuehnle et al. · 2016 [cited by applicant]
US 20160075989A1 · Carberry et al. · 2016 [cited by applicant]
US 20160090317A1 · Ju et al. · 2016 [cited by applicant]
US 20170233272A1 · Chidambaran et al. · 2017 [cited by applicant]
US 20170321181A1 · Hazlebeck et al. · 2017 [cited by applicant]
US 20180171275A1 · Wen et al. · 2018 [cited by applicant]
US 20180201887A1 · Gross et al. · 2018 [cited by applicant]
US 20190248688A1 · Wen et al. · 2019 [cited by applicant]
US 20200022384A1 · Gross et al. · 2020 [cited by applicant]
US 20200024559A1 · Gross et al. · 2020 [cited by applicant]
US 20200048122A1 · Gross et al. · 2020 [cited by applicant]
US 20200123482A1 · Gross et al. · 2020 [cited by applicant]
US 20200231477A1 · Wen et al. · 2020 [cited by applicant]
CN 103289887A · 2013 [cited by applicant]
GB 2471492A · 2011 [cited by examiner]
WO 2010011320A1 · 2010 [cited by applicant]
WO 2010030953A2 · 2010 [cited by applicant]
Johnson et al., “Development of an attached microalgal growth system for biofuel production,” Applied Microbiology and Biotechnology (2010), 85:525-534, Jul. 7, 2009. [cited by applicant]
Bitog et al., “Application of computational fluid dynamics for modeling and designing photobioreactors for microalgae production: A review,” Computers and Electronics in Agriculture (2011), 76:131-147, Jan. 24, 2011. [cited by applicant]
Christenson et al. “Rotating Algal Biofilm Reactor and Spool Harvester for Wastewater Treatment with Biofuels By-Products,” Biotechnology and Bioengineering, DOI 10.1002/bit.24451 (2012) Wiley Periodicals, Inc. Jan. 20,… [cited by applicant]
International Search Report for International Application No. PCT/US2014/0299618, mailed Aug. 21, 2014. [cited by applicant]
Written Opinion of the International Searching Authority for International Application No. PCT/US2014/029618, mailed Aug. 21, 2014, 4 pages. [cited by applicant]
International Search Report for International Application No. PCT/US2020/014393 dated as mailed on Mar. 25, 2020; 10 pages. [cited by applicant]
Peng, et al., Removal of total dissolved solids from wastewater using a revolving algal biofilm reactor, Water Environment Research, Nov. 12, 2019 [retrieved on Mar. 1, 2020]. Retrieved from the Internet<URL: https://on… [cited by applicant]