Method for magnetically labeling particles and labeling apparatus
The present disclosure relates to a method for labeling particles with magnetic particles and an apparatus for labeling particles with magnetic particles.
1. An apparatus for labeling particles with magnetic beads, comprising:
a filter portion a filter having a capture surface;
an introduction flow channel through which a suspension comprising magnetic beads is introduced into the filter portion;
an ejection flow channel that holds or rejects liquid of the suspension that has passed through the filter portion;
a liquid feeding means that supplies the suspension to the filter portion such that the particles are captured on the capture surface of the filter; and
a control unit that controls the liquid feeding means,
wherein the control unit is configured to control the liquid feeding means so as to
create a state in which both the capture surface and the other surface being opposite to the capture surface are in contact with the suspension comprising the magnetic beads,
from the state in which both the capture surface and the other surface of the filter are in contact with the suspension comprising the magnetic beads, reversely feed the suspension comprising the magnetic beads through the filter from the other surface of the filter toward the capture surface of the filter, and
pass the suspension comprising unreacted magnetic beads through the filter from the capture surface of the filter toward the other surface of the filter so that the unreacted magnetic beads are removed and the particles labeled with the magnetic beads are captured on the capture surface of the filter.
2. The apparatus according to claim 1 , comprising:
a means for stirring liquid on the capture surface of the filter or in the vicinity of the capture surface of the filter.
3. The apparatus according to claim 1 , further comprising:
a collection means for collecting the particles labeled with the magnetic beads captured in the filter portion with a collection liquid,
wherein the control unit controls the collection means so as to pass the collection liquid through the filter on which the particles labeled with the magnetic beads are captured, in a direction that is reverse of a direction in which the particles passes through the filter to be captured on the capture surface of the filter, and so as to collect the collection liquid staying on the filter through suction.
4. The apparatus according to claim 1 ,
wherein the control unit controls the liquid feeding means so as to supply the suspension to the filter portion such that the particles are captured on the capture surface of the filter.
5. The apparatus according to claim 4 ,
wherein the control unit controls the liquid feeding means so as to supply the suspension to the filter portion at a flow velocity of 0.01 mm/sec to 100 mm/sec per opening area of the filter.
6. The apparatus according to claim 4 ,
wherein the control unit controls the liquid feeding means such that a flow velocity of the reverse feeding of the suspension is faster than 1/10 the flow velocity of the supplying of the suspension to the filter portion.
7. The apparatus according to claim 4 ,
wherein the control unit controls the liquid feeding means so as to supply the suspension to the filter portion at a flow velocity of 0.07 mm/sec to 100 mm/sec per opening area of the filter.
8. The apparatus according to claim 4 ,
wherein the control unit controls the liquid feeding means so as to supply the suspension to the filter portion at a flow velocity of 0.1 mm/sec to 100 mm/sec per opening area of the filter.
9. The apparatus according to claim 4 ,
wherein the control unit controls the liquid feeding means so as to supply the suspension to the filter portion at a flow velocity of 0.3 mm/sec to 100 mm/sec per opening area of the filter.
10. The apparatus according to claim 1 ,
wherein the control unit controls the liquid feeding means such that the flow velocity of the reverse feeding of the suspension is 0.01 mm/sec or more per opening area of the filter.