Granted Patent B2

US 12,409,441 · App. 17/800,515 · Granted Sep 9, 2025

Inventors: James Wei Xu (Basking Ridge, NJ); Jiali Liao (San Ramon, CA); Christopher Belisle (Walnut Creek, CA); Fang Song (Wuxi, CN); Haihui Yuan (Nantong, CN); Juan You (Wuxi, CN); Wenli Cao (Wuxi, CN); Xiaozhong Bao (Wuxi, CN)

Assignee: Bio-Rad Laboratories, Inc.

B01J20/3204B01J20/286B01J20/3057B01J2220/86

Abstract

Claims (26)

1. A method of preparing a polymer-filled chromatography resin, the method comprising:

adding a chromatography resin to a solution comprising melted polymer while stirring to allow the melted polymer to absorb or otherwise enter the pores of the chromatography resin;

atomizing the solution and collecting insoluble porous polymer-filled chromatography resin beads in a water bath; and

removing excess polymer from the insoluble porous polymer-filled chromatography resin beads.

2. The method of claim 1 , wherein the removing step comprises passing the insoluble porous polymer-filled chromatography resin beads through one or more screens having openings from about 50 μm to about 200 μm.

3. The method of claim 1 , further comprising washing the insoluble porous polymer-filled chromatography resin beads.

4. The method of claim 1 , wherein the melted polymer is agarose and the agarose is in a concentration from about 0.5% to about 8% weight by volume.

5. The method of claim 1 , wherein the chromatography resin is a macroporous resin.

6. The method of claim 5 , wherein the macroporous resin is a hydroxyapatite microsphere.

7. The method of claim 5 , wherein the macroporous resin comprises acrylamide, methacrylate, polystyrene, or silica.

8. The method of claim 7 , wherein the macroporous resin comprises a copolymer of 3-allyloxy-1,2-propanediol and vinyl pyrrolidinone crosslinked with N,N′-methylenebisacrylamide.

9. The method of claim 7 , wherein the macroporous resin comprises glycidyl methacrylate and diethylene glycol dimethacrylate.

10. The method of claim 1 , wherein the melted polymer is at about 100° C.

11. The method of claim 1 , wherein atomizing the solution comprises surrounding the solution emerging from a nozzle with a stream of air.

12. The method of claim 11 , wherein a temperature of the stream of air is about 20° C. to about 90° C.

13. The method of claim 11 , wherein a flow rate of the.

14. The method of claim 1 , wherein a temperature of the water bath is about 4° C. to about 40° C.

15. A method of performing chromatography, the method comprising

contacting a sample comprising a target molecule to an insoluble porous polymer-filled chromatography resin prepared by the process of claim 1 under conditions such that the target molecule is not captured by the insoluble porous polymer-filled chromatography resin; and

collecting the target molecule from the insoluble porous polymer-filled chromatography resin.

16. The method of claim 15 , wherein the sample comprises a contaminant that is captured by the insoluble porous polymer-filled chromatography resin.

17. The method of claim 15 , wherein the target molecule is a protein-nanoparticle conjugate and the contaminant is free protein.

18. The method of claim 15 , wherein the sample comprises a protein-nanoparticle conjugate, free protein, and a buffer.

19. The method of claim 18 , wherein the sample further comprises a surfactant.

20. The method of claim 19 , wherein the surfactant is polyalkylene glycol.

21. The method of claim 20 , wherein the surfactant is Pluronic F-68.

Assignments (1)

Continuity (1)

References Cited (67)